干旱区研究

干旱区研究 ›› 2018, Vol. 35 ›› Issue (4): 977-983.doi: 10.13866/j.azr.2018.04.28

• 其他 • 上一篇    下一篇

荒漠草原植物骆驼蓬根际土壤细菌群落分析

程琳1, 陈吉祥1, 李彦林1, 王永刚1, 周永涛2, 张彦2   

  1. 1.兰州理工大学石油化工学院,甘肃 兰州 730050;
    2.中石油北京天然气管道有限公司,北京 100101
  • 收稿日期:2017-05-04 修回日期:2018-04-10 出版日期:2018-07-15 发布日期:2025-11-18
  • 通讯作者: 陈吉祥. E-mail: chenjixiang@lut.cn
  • 作者简介:程琳(1992-),男,硕士研究生,主要研究方向为环境微生物技术. E-mail: czlin566@163.com
  • 基金资助:
    国家自然科学基金(31272694);中国石油天然气股份有限公司天然气与管道分公司科研项目(2014D-4610-0501)

Diversity of Bacterial Communities in Rhizosphere Soil of Peganum harmala L.in Desert Steppe

CHENG Lin1, CHEN Ji-xiang1, LI Yan-lin1, WANG Yong-gang1, ZHOU Yong-tao2, ZHANG Yan2   

  1. 1. School of Petrochemical Engineering,Lanzhou University of Technology,Lanzhou 730050,Gansu,China;
    2. Beijing Natural Gas Pipeline Co. Ltd.,PetroChina,Beijing 100101,China
  • Received:2017-05-04 Revised:2018-04-10 Published:2018-07-15 Online:2025-11-18

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摘要： 骆驼蓬(Peganum harmala L.)具有较强耐旱抗寒特性,在维护生态环境中发挥着重要作用。为了解其生态适应及与环境相互作用机制,采用高通量测序和纯培养方法研究了甘肃白银荒漠草原区骆驼蓬根际土壤细菌群落特征,并与周边土壤进行了对比。结果表明:骆驼蓬根际土壤主要优势细菌群为放线菌门(Actinobacteria)30.01%、变形菌门(Proteobacteria)23.98%、拟杆菌门(Bacteroidetes)11.53%、酸杆菌门(Acidobacteria)10.19%。周边荒漠草原土壤主要优势细菌群为放线菌门55.05%、变形菌门21.11%、酸杆菌门6.07%。可培养细菌类群包括厚壁菌门(Firmicutes)、变形菌门、放线菌门,其中根际土壤分离的优势菌为短杆菌属(Brevibacterium)23.53%、芽孢杆菌属(Bacillus)23.53%、假单胞菌属(Pseudomonas)17.65%,周边土壤可培养细菌的优势种群为节杆菌属(Arthrobacter)37.50%、芽孢杆菌属18.75%、假单胞菌属12.50%。骆驼蓬根际微生物群落组成和结构与周边土壤有显著差异,根际土壤微生物数量及多样性指数明显高于非根际土壤。研究结果对了解荒漠植物与根际微生物之间相互作用,揭示土壤微生物促进植物根际土壤物质循环转化作用,筛选促生长有益微生物,为加强荒漠草原生态保护提供科学依据。

关键词: 荒漠草原, 骆驼蓬(Peganum harmala L.), 根际土壤, 细菌群落, 高通量测序, 土壤微生物

Abstract: Peganum harmala L.is strong in resistance against severe environment,such as drought and low temperature.Plants play an important role in maintaining the local ecological environment.In order to understand the mechanism of ecological adaptation and interaction with environment,the characteristics of bacterial communities in rhizosphere soil of P.harmala in a desert steppe in Baiyin,Gansu Province were studied using the high-throughput sequencing and culture-dependent method,and they were compared with those of surrounding soil.The results showed that the bacterial communities in the rhizosphere soil were consisted of 30.01% Actinobacteria,23.98% Proteobacteria,11.53% Bacteroidetes and 10.19% Acidobacteria.The dominant soil bacterial community in the surrounding desert steppe was composed of 55.05% Actinobacteria,21.11% Proteobacteria and 6.07% Acidobacteria.The cultivable bacterium group included Firmicutes,Proteobacteria and Actinobacteria.In the rhizosphere soil,the dominant bacteria were 23.53% Brevibacterium, 23.53% Bacillus,17.65% Pseudomonas and 11.76% Brevundimonas. The dominant populations of bacteria in the surrounding soil were 37.50% Arthrobacter, 18.75% Bacillus and 12.50% Pseudomonas. The difference of bacterial communities between the rhizosphere soil of P.harmala and its surrounding desert steppe soil was significant.The bacteria numbers and diversity index in the rhizosphere soil were significantly higher than those of the surrounding desert steppe soil.The results provided the scientific basis for exploring the interaction between the desert steppe plants and their rhizobacteria,and also for understanding the roles of the rhizobacteria in promoting plant growth and soil material transformation.

Key words: desert steppe, Peganum harmala L., rhizosphere soil, bacterial community, high throughput sequencing, soil microorganism