呼伦贝尔沙地樟子松人工林土壤细菌网络特征

doi:10.13866/j.azr.2023.06.06

干旱区研究 ›› 2023, Vol. 40 ›› Issue (6): 905-915.doi: 10.13866/j.azr.2023.06.06

呼伦贝尔沙地樟子松人工林土壤细菌网络特征

张颂安¹(),刘轩¹,赵珮杉¹,高广磊^1,^2,³(),张英^1,³,丁国栋^1,^2,³,柳叶¹,任悦¹

1.北京林业大学水土保持学院，林业生态工程教育部工程研究中心，北京 100083
2.宁夏盐池毛乌素沙地生态系统国家定位观测研究站，宁夏盐池 751500
3.水土保持国家林业和草原局重点实验室，北京 100083

收稿日期:2022-09-21 修回日期:2022-12-07 出版日期:2023-06-15 发布日期:2023-06-21
通讯作者: 高广磊. E-mail: gaoguanglei@bjfu.edu.cn
作者简介:张颂安（1997-），女，硕士研究生，主要研究方向为荒漠化防治. E-mail: zhangsongan@bjfu.edu.cn
基金资助:
内蒙古自治区科技计划项目(2022YFHH0131);中央高校基本科研业务费项目(2021ZY47)

Soil bacterial networks in Pinus sylvestris var. mongolica plantations of the Hulunbuir Desert

ZHANG Songan¹(),LIU Xuan¹,ZHAO Peishan¹,GAO Guanglei^1,^2,³(),ZHANG Ying^1,³,DING Guodong^1,^2,³,LIU Ye¹,REN Yue¹

1. School of Soil and Water Conservation, Beijing Forestry University, Engineering Research Center of Forestry Ecological Engineering, Ministry of Education, Beijing 100083, China
2. Yanchi Ecology Research Station of the Mu Us Desert, Yanchi 751500, Ningxia, China
3. Key Laboratory of State Forestry and Grassland Administration on Soil and Water Conservation, Beijing 100083, China

Received:2022-09-21 Revised:2022-12-07 Online:2023-06-15 Published:2023-06-21

摘要/Abstract

摘要：

为揭示呼伦贝尔沙地樟子松人工林土壤细菌相互关系，以呼伦贝尔沙地不同林龄樟子松人工林（25 a、34 a和43 a）为研究对象，以沙质草地为对照，采用分子生态网络分析法对不同土层（0~10 cm和10~20 cm）土壤细菌群落进行比较分析。结果表明：（1）从25 a到43 a，土壤细菌网络总边数增多，平均路径长度降低。土壤深度由0~10 cm到10~20 cm，人工林土壤细菌网络总边数减少，平均路径长度升高。与沙质草地相比，人工林土壤细菌网络总边数较少。（2） 25 a人工林关键菌种隶属于嗜酸菌目（Acidimicrobiales）、RB41和MB-A2-108，34 a人工林关键菌种隶属于Gaiellales，43 a人工林关键菌种隶属于Gaiellales、RB41、Subgroup_7、Subgroup_6、和DA101_soil_group，草地关键菌种隶属于匿杆菌门（Latescibacteria）。（3）全氮、氨氮、微生物碳含量和脲酶酶活性对土壤细菌网络中具有高中介中心性的部分细菌有显著正相关影响（P<0.05）；转化酶和过氧化氢酶活性、土壤含水量以及速效磷含量对土壤细菌网络中具有高中介中心性的部分细菌有显著负相关影响（P<0.05）；土壤有机质对土壤细菌网络中具有高中介中心性的部分细菌既有显著正相关影响又有显著负相关影响（P<0.05）。樟子松人工林从25 a到43 a，土壤细菌网络愈加复杂和紧密，土壤深度由0~10 cm到10~20 cm，网络复杂性和紧密度降低；与草地相比，人工林土壤细菌网络复杂性较低。43 a人工林土壤细菌网络关键菌种类型数量最多。另外，土壤细菌网络受土壤有机质影响最大。研究结果有助于深入理解呼伦贝尔沙地樟子松人工林土壤细菌群落，并为呼伦贝尔沙地樟子松人工林的可持续经营提供科技支撑。

关键词: 土壤微生物, 分子生态网络, 林龄, 土层, 樟子松, 呼伦贝尔沙地

Abstract:

To elucidate soil bacterial network interactions within Pinus sylvestris var. mongolica plantations in the Hulunbuir Desert. P. sylvestris plantations representing three different age groups (25 a, 34 a, and 43 a) and two soil layers (0-10 and 10-20 cm) were selected to assess their soil bacterial networks using molecular ecological network analysis and data from a referenced grassland. The numbers of network edges increased, the average path length reduced from 25 a to 43 a. While the number of network edges reduced and the average path length increased with soil layers from 0-10 cm to 10-20 cm. Compared with the grassland, the P. sylvestris plantations had a lower network edge, and the soil bacterial network was less complicated. The soil bacteria were found to belong to the Acidimicrobiales, RB41, and MB-A2-108 in the 25 a plantation, Gaiellales in the 34 a plantation, and Gaiellales, RB41, Subgroup_7, Subgroup_6, and DA101_soil_group in the 43 a plantation, Latescibacteria in the grassland. The soil bacterial network was significantly positively correlated with ammonia nitrogen, total nitrogen, microbial carbon content, and urease activities and significantly negatively correlated with invertase and catalase activities, soil water content and available phosphorus (P < 0.05). The soil organic matter had both positive and negative effects (P < 0.05). The soil bacterial network complexity and compactness increased from 25 a to 43 a. The opposite was found for the soil layers from 0-10 cm to 10-20 cm. Compared with the grassland, the soil bacterial network of the P. sylvestris plantation was less complicated. The keystone soil bacteria species were different among the three stand ages, and were greatest in stand 43 a. There were more keystone species in the plantation than the grassland. Soil physicochemical properties and enzymatic activity derived the soil bacterial network, and soil organic matter was the major influencing factor. This improved information contributed to a deep understanding of the soil bacterial community and provided a scientific and technological basis for the sustainable management of P. sylvestris plantations in the Hulunbuir Desert.

Key words: soil microbes, molecular ecological network, stand age, soil depth, Pinus sylvestris var. mongolica, the Hulunbuir Desert

张颂安, 刘轩, 赵珮杉, 高广磊, 张英, 丁国栋, 柳叶, 任悦. 呼伦贝尔沙地樟子松人工林土壤细菌网络特征[J]. 干旱区研究, 2023, 40(6): 905-915.

ZHANG Songan, LIU Xuan, ZHAO Peishan, GAO Guanglei, ZHANG Ying, DING Guodong, LIU Ye, REN Yue. Soil bacterial networks in Pinus sylvestris var. mongolica plantations of the Hulunbuir Desert[J]. Arid Zone Research, 2023, 40(6): 905-915.

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样地编号	林龄/a	平均树高/m	平均胸径/cm	林分密度/（株·hm^-2）	郁闭度	土壤类型
HLP25	25	12.16±1.21	15.24±2.79	1650	0.76	风沙土
HLP34	34	12.71±0.97	19.25±3.10	1650	0.84	风沙土
HLP43	43	13.52±1.24	23.59±3.28	1650	0.73	风沙土
HLG	-	-	-	-	0.75	风沙土

网络节点	模块内连通度（Zi）	模块间连通度（Pi）
模块中心点	>2.5	<0.62
连接节点	<2.5	>0.62
网络中心点	>2.5	>0.62
外围节点	<2.5	<0.62

拓扑特征		HLP25A	HLP25B	HLP34A	HLP34B	HLP43A	HLP43B	HLGA	HLGB
经验网络	节点	306	304	307	292	300	268	330	325
	边	2395	2262	2483	2313	3072	2011	2689	2831
	平均连通度	15.65	14.88	16.18	15.84	20.48	15.01	16.30	17.42
	模块化	0.75	0.77	0.70	0.72	0.62	0.62	0.71	0.71
	平均路径长度	11.89	15.26	11.50	11.67	8.57	13.81	12.14	11.52
	平均聚类系数	0.75	0.74	0.75	0.75	0.76	0.74	0.74	0.75
	正相关/%	54.53	51.50	52.56	49.72	52.96	50.22	48.83	53.27
	负相关/%	45.47	48.50	47.44	50.28	47.04	49.78	51.17	46.73
随机网络	模块化	0.21	0.21	0.20	0.20	0.18	0.21	0.21	0.20
	平均路径长度	2.37	2.41	2.35	2.34	2.16	2.35	2.37	2.32
	平均聚类系数	0.05	0.05	0.06	0.05	0.07	0.05	0.05	0.05

关键菌种	OTU	门	纲	目	科	属
HLP25A	OTU144	Actinobacteria	Acidimicrobiia	Acidimicrobiales	-	-
HLP25B	OTU182	Acidobacteria	Blastocatellia	Blastocatellales	Blastocatellaceae_Subgroup_4	RB41
HLP25B	OTU291	Actinobacteria	MB-A2-108	-	-	-
HLP34A	OTU149	Actinobacteria	Thermoleophilia	Gaiellales	-	-
HLP34B	-	-	-	-	-	-
HLP43A	OTU66	Acidobacteria	Blastocatellia	Blastocatellales	Blastocatellaceae_Subgroup_4	RB41
	OTU677	Acidobacteria	Holophagae	Subgroup_7	-	-
	OTU319	Acidobacteria	Subgroup_6	-	-	-
	OTU556	Verrucomicrobia	Spartobacteria	Chthoniobacterales	DA101_soil_group	-
HLP43B	OTU152	Acidobacteria	Subgroup_6	-	-	-
HLP43B	OTU3844	Acidobacteria	Thermoleophilia	Gaiellales	-	-
HLGA	-	-	-	-	-	-
HLGB	OTU718	Latescibacteria	-	-	-	-

土壤因子	HLP25A	HLP25B	HLP34A	HLP34B	HLP43A	HLP43B	HLGA	HLGB
土壤含水量SWC/%	10.44±3.66a	5.77±1.39A	9.00±1.27a	6.66±0.78A	6.88±0.47b	5.81±2.08A	10.18±0.89a	7.69±1.35A
全氮TN/（g·kg^-1）	0.82±0.01b	0.56±0.01C	0.77±0.01c	0.63±0.00A	0.51±0.03d	0.49±0.01D	0.93±0.02a	0.59±0.01B
土壤有机质SOM /（g·kg^-1）	2.31±0.06a	1.90±0.05A	2.11±0.05b	1.82±0.02AB	1.71±0.12c	1.32±0.06C	2.01±0.08b	1.74±0.06B
氨氮AN/（mg·kg^-1）	45.37±3.08ab	84.73±1.59A	43.30±2.19b	44.57±2.99B	50.06±3.36a	30.63±5.17C	49.82±3.67a	44.12±0.57B
速效磷AP/（g·kg^-1）	3.87±0.31a	3.35±0.51A	6.11±1.44a	4.93±0.30A	5.40±1.96a	5.07±0.84A	4.13±0.65a	3.28±1.86A
过氧化氢酶CA/（U·g^-1）	938.76±31.28b	545.09±528.63AB	132.00±38.11c	172.64±7.02B	997.95±4.60a	921.19±29.80A	992.65±22.58a	884.29±25.03A
转化酶IA/（U·g^-1）	70.92±6.00c	41.24±2.36B	69.63±1.11c	41.64±7.94B	97.93±3.00a	48.94±6.24B	81.17±4.79b	74.61±8.92A
脲酶SU/（U·g^-1）	635.42±35.36a	213.98±68.25A	396.04±43.47c	251.52±20.26A	511.12±26.12b	223.20±162.05A	155.32±28.05d	258.03±26.61A
微生物碳MC /（mg·kg^-1）	21.58±12.89c	18.52±14.02B	73.09±1.78a	44.89±2.15A	14.32±1.74c	11.60±3.30B	59.32±4.63b	51.75±1.16A

呼伦贝尔沙地樟子松人工林土壤细菌网络特征

Soil bacterial networks in Pinus sylvestris var. mongolica plantations of the Hulunbuir Desert

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