干旱区研究 ›› 2023, Vol. 40 ›› Issue (2): 194-202.doi: 10.13866/j.azr.2023.02.04
张彤1,2(),刘静1,2,韩叙1,2,童郁强1,2,魏亚伟1,2()
收稿日期:
2022-07-04
修回日期:
2022-10-21
出版日期:
2023-02-15
发布日期:
2023-03-08
通讯作者:
魏亚伟
作者简介:
张彤(1997-),女,硕士研究生,主要从事森林土壤研究. E-mail: 基金资助:
ZHANG Tong1,2(),LIU Jing1,2,HAN Xu1,2,TONG Yuqiang1,2,WEI Yawei1,2()
Received:
2022-07-04
Revised:
2022-10-21
Online:
2023-02-15
Published:
2023-03-08
Contact:
Yawei WEI
摘要:
为探究放牧对土壤养分和微生物群落的影响,本研究分别选取了放牧和围封沙地樟子松林为研究对象,基于高通量测序技术,并测定土壤化学指标,分析放牧与围封土壤中养分、微生物群落组成以及多样性的差异。研究结果显示:(1) 放牧后全磷含量显著降低(P<0.05),土壤有机碳和全氮含量也降低,但差异并不显著(P>0.05)。(2) 从土壤微生物群落组成上来看,真菌分类学门水平中担子菌门比例放牧显著低于围封(P<0.05),但对细菌门水平并未产生显著影响(P>0.05)。(3) 就土壤微生物多样性而言,放牧极显著降低了细菌的Chao1指数和Shannon指数(P<0.01),对真菌群落多样性无明显影响(P>0.05),可见,放牧对土壤细菌多样性指数的影响较真菌更大。综上所述,沙地樟子松林的放牧行为对土壤养分及土壤微生物均有不同程度的负面影响,所以该地区应适度减少放牧行为,缓解土地压力,以保障樟子松人工林的可持续利用。
张彤,刘静,韩叙,童郁强,魏亚伟. 放牧对沙地樟子松林土壤养分及微生物群落的影响[J]. 干旱区研究, 2023, 40(2): 194-202.
ZHANG Tong,LIU Jing,HAN Xu,TONG Yuqiang,WEI Yawei. Effects of grazing on soil nutrients and microbial community of Pinus sylvestris var. mongolica forest in sandy land[J]. Arid Zone Research, 2023, 40(2): 194-202.
表3
各分类水平微生物类群数统计"
土壤微生物 | 处理 | 界 | 门 | 纲 | 目 | 科 | 属 | 种 |
---|---|---|---|---|---|---|---|---|
真菌 | CK | 1 | 7.6±0.89a | 20.6±3.97a | 47.4±7.77a | 78.8±13.18a | 116.4±26.88a | 101.2±26.24a |
G | 1 | 7.2±0.84a | 18.8±2.17a | 40.6±3.51a | 64.2±5.45a | 88.8±10.38a | 74.6±10.41a | |
细菌 | CK | 1 | 26.8±2.59a | 76.0±5.24a | 159.4±10.06a | 250.0±11.92a | 371.6±25.14a | 99.8±18.78a |
G | 1 | 25.8±2.17a | 74.0±2.92a | 151.8±9.83a | 233.2±12.89a | 332.6±20.39b | 83.0±11.16a |
[1] | 于东伟, 雷泽勇, 赵国军, 等. 樟子松固沙林土壤理化特性对林分密度的响应[J]. 干旱区研究, 2020, 37(1): 134-141. |
[Yu Dongwei, Lei Zeyong, Zhao Guojun, et al. Response of soil physicochemical properties under stand-fixation forest of Pinus sylvestris var. mongolica to stand density[J]. Arid Zone Research, 2020, 37 (1): 134-141.] | |
[2] | 石柯, 董士刚, 申凤敏, 等. 小麦播量与减氮对潮土微生物量碳氮及土壤酶活性的影响[J]. 中国农业科学, 2019, 8(15): 2646-2663. |
[Shi Ke, Dong Shigang, Shen Fengmin, et al. Effects of wheat seeding rate with nitrogen fertilizer application reduction on soil microbial biomass carbon, nitrogen and enzyme activities in fluvo-aquic soil in Huang-Huai Plain[J]. Scientia Agricultura Sinica, 2019, 8(15): 2646-2663.] | |
[3] |
Liu T, Wu X, Li H, et al. Soil organic matter, nitrogen and pH driven change in bacterial community following forest conversion-Science Direct[J]. Forest Ecology and Management, 2020, 477: 118473.
doi: 10.1016/j.foreco.2020.118473 |
[4] | 周恒, 周会程, 肖海龙, 等. 三江源不同海拔高寒草原表层土壤酶活性特征[J]. 草原与草坪, 2019, 3(5): 20-30. |
[Zhou Heng, Zhou Huicheng, Xiao Hailong, et al. Characteristics of enzyme activity in surface soil of alpine steppe under different altitudes on Qinghai-Tibetan Plateau[J]. Grassland and Turf, 2019, 3(5): 20-30.] | |
[5] |
马源, 李林芝, 张德罡, 等. 退化高寒草甸优势植物根际与非根际土壤养分及微生物量的分布特征[J]. 草地学报, 2019, 27(4): 797-804.
doi: 10.11733/j.issn.1007-0435.2019.04.002 |
[Ma Yuan, Li Linzhi, Zhang Degang, et al. Distribution characteristics of nutrients and microbial biomass in rhizosphere and non-rhizosphere soils of dominant plants in degraded alpine meadow[J]. Acta Agrestia Sinica, 2019, 27(4): 797-804.]
doi: 10.11733/j.issn.1007-0435.2019.04.002 |
|
[6] | 亓琳, 李艳玲, 赵威, 等. 锶污染下燕麦对土壤酶活性和微生物群落功能多样性的影响[J]. 生态学报, 2017, 38(13): 4888-4896. |
[Qi lin, Li Yanling, Zhao Wei, et al. Effect of Avena sativa L. on soil enzyme activity and microbe functional diversity under strontium pollution[J]. Acta Ecologica Sinica, 2017, 38(13): 4888-4896.] | |
[7] | 郭辉, 唐卫平. 不同林龄华北落叶松根际与非根际土壤酶和土壤微生物研究[J]. 环境生态学报, 2020, 29(11): 2163-2170. |
[Guo Hui, Tang Weiping. Enzyme activity and microbial community diversity in rhizosphere and non-rhizosphere soil of Larix principis-rupprechtii[J]. Ecology and Environmental Sciences, 2020, 29 (11): 2163-2170.] | |
[8] | 于德良, 雷泽勇, 张岩松, 等. 沙地樟子松人工林土壤酶活性及其影响因子[J]. 干旱区研究, 2019, 2(3): 621-629. |
[Yu Deliang, Lei Zeyong, Zhang Yansong, et al. Soil enzyme activity and its affecting factors under Pinus sylvestris var. mongolica plantation in sandy land[J]. Arid Zone Research, 2019, 2(3): 621-629.] | |
[9] |
丁成翔, 杨晓霞, 董全民. 青藏高原高寒草原放牧方式对植被、土壤及微生物群落的影响[J]. 草地学报, 2020, 28(1): 159-169.
doi: 10.11733/j.issn.1007-0435.2020.01.018 |
[Ding Chengxiang, Yang Xiaoxia, Dong Quanmin. Effects of grazing patterns on vegetation, soil and microbial community in alpine grassland of Qinghai-Tibetan Plateau[J]. Acta Agrestia Sinica, 2020, 28(1): 159-169.]
doi: 10.11733/j.issn.1007-0435.2020.01.018 |
|
[10] | 杨阳, 贾丽欣, 乔荠瑢, 等. 重度放牧对荒漠草原土壤养分及微生物多样性的影响[J]. 中国草地学报, 2019, 41(4): 72-79. |
[Yang Yang, Jia Lixin, Qiao Qirong, et al. Effects of heavy grazing on soil nutrients and microbial diversity in desert steppe[J]. Chinese Journal of Grassland, 2019, 41(4): 72-79.] | |
[11] |
高昌宇, 齐志远, 郑慧, 等. 土壤有效养分和微生物特征对短期不同放牧强度的响应[J]. 草地学报, 2022, 30(7): 1641-1650.
doi: 10.11733/j.issn.1007-0435.2022.07.004 |
[Gao Changyu, Qi Zhiyuan, Zheng Hui, et al. Responses of soil available nutrients and microbial characteristics to short-term grazing intensities[J]. Acta Agrestia Sinica, 2022, 30(7): 1641-1650.]
doi: 10.11733/j.issn.1007-0435.2022.07.004 |
|
[12] | 马香云, 王俊杰. 放牧对人工草地群落特征及土壤养分的影响[J]. 草原与草业, 2019, 31(4): 19-26. |
[Ma Xiangyun, Wang Junjie. Effects of grazing on community characteristics and soil nutrients of artificial grassland[J]. Grassland and Prataculture, 2019, 31(4): 19-26.] | |
[13] | 祁正超, 常佩静, 李永善, 等. 放牧对荒漠灌丛草地土壤团聚体组成及其稳定性的影响[J]. 干旱区研究, 2021, 38(1): 87-94. |
[Qi Zhengchao, Chang Peijing, Li Yongshan, et al. Effects of grazing intensity on soil aggregates compositi, stability, nutrients and C/N in desert shrubland[J]. Arid Zone Research, 2021, 38(1): 87-94.] | |
[14] |
Thomas W, Stephen M, Daniel S, et al. Biotic interactions mediate soil microbial feedbacks to climate change[J]. Proceedings of the National Academy of Sciences of the United States of America, 2015, 112(22): 7033-7038.
doi: 10.1073/pnas.1502956112 pmid: 26038557 |
[15] |
Fanin N, Gundale M J, Farrell M, et al. Consistent effects of biodiversity loss on multifunctionality across contrasting ecosystems[J]. Nature Ecology and Evolution, 2018, 2: 269-278.
doi: 10.1038/s41559-017-0415-0 pmid: 29255299 |
[16] |
M Delgado-Baquerizo, Giaramida L, Reich P B, et al. Lack of functional redundancy in the relationship between microbial diversity and ecosystem functioning[J]. Journal of Ecology, 2016, 104(4): 936-946.
doi: 10.1111/1365-2745.12585 |
[17] |
Tian C, Chen J, Yang Z. Phylogenetic diversity of microbes and its perspectives in conservation biolog[J]. Plant and Soil, 2016, 407(1): 1-15
doi: 10.1007/s11104-016-3040-9 |
[18] |
Osono T. Ecology of ligninolytic fungi associated with leaf litter decomposition[J]. Ecological Research, 2007, 22(6): 955-974.
doi: 10.1007/s11284-007-0390-z |
[19] |
Lienhard P, Terrat S, Prévost-Bouré N C, et al. Pyrosequencing evidences the impact of cropping on soil bacterial and fungal diversity in Laos tropical grassland[J]. Agronomy for Sustainable Development, 2014, 34(2): 525-533.
doi: 10.1007/s13593-013-0162-9 |
[20] |
Wang M, Wang S, Wu L, et al. Evaluating the lingering effect of livestock grazing on functional potentials of microbial communities in Tibetan grassland soils[J]. Plant and Soil, 2016, 407: 385-399.
doi: 10.1007/s11104-016-2897-y |
[21] |
尹亚丽, 王玉琴, 李世雄, 等. 围封对退化高寒草甸土壤微生物群落多样性及土壤化学计量特征的影响[J]. 应用生态学报, 2019, 30(1): 127-136.
doi: 10.13287/j.1001-9332.201901.009 |
[Yin Yali, Wang Yuqin, Li Shixiong, et al. Effects of enclosing on soil microbial community diversity and soil stoichiometric characteristics in a degraded alpine meadow[J]. Chinese Journal of Applied Ecology, 2019, 30(1): 127-136.]
doi: 10.13287/j.1001-9332.201901.009 |
|
[22] | 杜宇凡, 古琛, 王亚婷, 等. 放牧率对短花针茅根际和非根际土壤氮素的影响[J]. 草业科学, 2016, 33(6): 1021-1027. |
[Du Yufan, Gu Chen, Wang Yating, et al. Effects of grazing on nitrogen contents in rhizosphere and non-rhizosphere soil of Stipa breviflora[J]. Pratacultural Science, 2016, 33 (6): 1021-1027.] | |
[23] | Li Y, Wang S, Jiang L, et al. Changes of soil microbial community under different degraded gradients of alpine meadow[J]. Agriculture, Ecosystems & Environmen, 2016, 222: 213-222. |
[24] |
王天乐, 卫智军, 刘文亭, 等. 不同放牧强度下荒漠草原土壤养分和植被特征变化研究[J]. 草地学报, 2017, 25(4): 711-716.
doi: 10.11733/j.issn.1007-0435.2017.04.004 |
[Wang Tianle, Wei Zhijun, Liu Wenting, et al. Study on changes of soil nutrients and plant community of Stipa breviflora steppe under different grazing intensities[J]. Acta Agrestia Sinica, 2017, 25(4): 711-716.]
doi: 10.11733/j.issn.1007-0435.2017.04.004 |
|
[25] |
Mcsherry M E, Ritchie M E. Effects of grazing on grassland soil carbon: a global review[J]. Global Change Biology, 2013, 19(5): 1347-1357.
doi: 10.1111/gcb.12144 pmid: 23504715 |
[26] |
Ashworth A J, Debruyn J M, Allen F L, et al. Microbial community structure is affected by cropping sequences and poultry litter under long-term no-tillage[J]. Soil Biology and Biochemistry, 2017, 114: 210-219.
doi: 10.1016/j.soilbio.2017.07.019 |
[27] |
Walker T, Christina K, Florian S, et al. Microbial temperature sensitivity and biomass change explain soil carbon loss with warming[J]. Nature Climate Change, 2018, 8: 885-889.
doi: 10.1038/s41558-018-0259-x pmid: 30288176 |
[28] | 侍世玲, 任晓萌, 张晓伟, 等. 库布齐沙漠沙枣防护林土壤养分及化学计量特征[J]. 干旱区研究, 2022, 39(2): 469-476. |
[Shi Shiling, Ren Xiaomeng, Zhang Xiaowei, et al. Soil nutrients and stoichiometric characteristics of the Elaeagnus angustifolia shelterbelt in the Hobq Desert[J]. Arid Zone Research, 2022, 39(2): 469-476.] | |
[29] | 王利彦, 周国娜, 朱新玉, 等. 凋落物对土壤有机碳与微生物功能多样性的影响[J]. 生态学报, 2021, 41(7): 2709-2718. |
[Wang Liyan, Zhou Guona, Zhu Xinyu, et al. Effects of litter on soil organic carbon and microbial functional diversity[J]. Acta Ecologica Sinica, 2021, 41(7): 2709-2718.] | |
[30] | 杨思维, 张德罡, 牛钰杰, 等. 短期放牧对高寒草甸表层土壤入渗和水分保持能力的影响[J]. 水土保持学报, 2016, 30(4): 96-101. |
[Yang Siwei, Zhang Degang, Niu Yujie, et al. Effect of short-term grazing on surface soil infiltration and soil water retention in alpine meadow[J]. Journal of Soil and Water Conservation, 2016, 30(4): 96-101.] |
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