干旱区研究

干旱区研究 ›› 2012, Vol. 29 ›› Issue (5): 816-819.

• 植物生理 • 上一篇    下一篇

荒漠念珠藻对UV-B辐射的反应及外源抗氧化剂的保护作用

邓松强1, 杨翠琨2, 安晓亮3, 胡超珍1, 陈兰洲1   

  1. 1. 武汉大学资源与环境科学学院，湖北 武汉 430072;  2. 达拉特旗国营白土梁农场，内蒙古 达拉特旗 014300; 
    3. 内蒙古自治区林业科学研究院，内蒙古 呼和浩特 010020
  • 收稿日期:2011-11-09 修回日期:2012-02-15 出版日期:2012-09-15 发布日期:2012-10-09
  • 通讯作者: 陈兰洲.E-mail:chenlanzhou@yahoo.com.cn
  • 作者简介:邓松强（1987-）, 湖南益阳人，硕士研究生，主要从事于植物生理生态学研究. E-mail:izzzydeng1987@gmail.com
  • 基金资助:

    武汉市科技晨光计划项目(200850731367)；国家自然科学基金（30970446，30500068）资助

Response of Nostoc sp. to UV-B Radiation and the Protective Effect of Exogenous Antioxidants to Nostoc sp.

 DENG  Song-Qiang1, YANG  Cui-Kun2, AN  Xiao-Liang3, HU  Chao-Zhen1, CHEN  Lan-Zhou1   

  1. 1. College of Resources & Environmental Sciences, Wuhan University, Wuhan 430072, China;
    2. Baituliang State Farm, Dalat Banner 014300, Inner Mongolia, China;
    3. Inner Mongolia Academy of Forestry, Hohhot 010020, China
  • Received:2011-11-09 Revised:2012-02-15 Online:2012-09-15 Published:2012-10-09

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摘要： 通过分析UV-B辐射对荒漠结皮中的藻类——Nostoc sp.生物学效应的影响，以及外源抗氧化剂对UV-B辐射下藻类的保护作用。结果表明：Nostoc sp.的光合活性[WTBX](Fv/Fm)[WTBZ]随紫外处理强度的增大和处理时间的延长而显著降低，叶绿素荧光产量和相应光合参数（ET0/ABS、ET0/TR0和RC/CS0）降低，活性氧ROS（reactive oxygen evolution）产量显著增加，光合活性降低。而外源抗坏血酸（ASA）和半胱氨酸（NAC）的加入，可以显著提高紫外辐射条件下藻体的光合活性，降低ROS含量，从而有效保护藻类，且抗坏血酸的效果要优于半胱氨酸。实验结果表明：处于藻类结皮上层的Nostoc sp.有较强的对UV-B辐射的抗性，而外源抗氧化剂可以有效保护藻体免受紫外辐射的伤害。

关键词: UV 辐射, 荒漠, 生物结皮, 蓝藻, 光合活性, 活性氧

Abstract: In this paper, the effects of UV-B radiation on the physiological and biochemical processes of [WTBX]Nostoc[WTBZ] sp., one of cyanobacteria isolated from desert algal crust, were investigated, and the protective effects of ascorbic acid and N-acetyl-L-cysteine were researched. The results show that the PSII activity [WTBX](Fv/Fm)[WTBZ] of stressed cells was significantly decreased with the increase of irradiance time and intensity, the chlorophyll fluorescence transits and photosynthetic parameters （ET0/ABS、ET0/TR0 and RC/CS0）] were also decreased significantly, but the ROS generation was increased. Exogenous 0.5 mM ascorbic acid or N-acetyl-L-cysteine could significantly decrease the production of ROS (reactive oxygen evolution), and the protective effect of ascorbic acid was better than that of N-acetyl-L-cysteine. The results suggested that Nostoc sp. adapted to the detrimental UV environment in desert, and exogenous antioxidant could effectively protect cells from UV-B damage.

Key words: UV-B radiation, desert, biological crusts, cyanobacterium, photosynthesis efficiency, reactive oxygen species (ROS)