土壤水分胁迫对紫花苜蓿光合特性及其生物量的影响

摘要/Abstract

摘要： 紫花苜蓿是我国重要的豆科牧草，具有较强的抗旱能力，而水分亏缺是影响其产量的主要逆境因子之一。通过大田试验对不同水分胁迫下紫花苜蓿的光合特性及产量进行研究，结果表明：① 土壤水分胁迫对紫花苜蓿光响应参数影响显著(P＜0.05)，随着水分胁迫的加剧，最大净光合速率、表观量子效率、光饱和点逐渐降低，暗呼吸速率、光补偿点逐渐升高，从而直接影响紫花苜蓿光合作用对弱光的吸收和转化效率。② 随着水分胁迫的加剧紫花苜蓿叶片的净光合速率(Pn)和蒸腾速率(Tr)呈显著的下降趋势，气孔导度(Gs)呈先下降后上升的趋势，表明轻度水分胁迫下气孔因素是Pn下降的主要因素，中度和重度水分胁迫下非气孔因素是Pn降低的主要因素。③ 当光合有效辐射(PAR)为1 200 μmol·m^-2·s^-1时，轻度水分胁迫的水分利用效率(WUE)显著大于充分灌溉的WUE (P<0.05)，表明适度水分胁迫可提高紫花苜蓿叶片的水分利用效率。④ 轻度水分胁迫与充分灌溉干草产量之间无显著差异，表明轻度水分胁迫能达到高产节水的目的。

关键词: 紫花苜蓿, 地下滴灌, 水分利用效率, 水分胁迫, 光合作用, 生物量, 永昌, 甘肃

Abstract: Medicago sativa is an important legume forage in China, and it is strong in drought resistance. However, water deficit is still the main stress factor affecting its yield. The photosynthetic characteristics and yield of M. sativa under different water stress conditions were studied by carrying out the field experiments. The results showed that: ① Soil water stress affected significantly the photoresponse parameters of M. sativa. With the increase of soil water stress, the maximum net photosynthetic rate, apparent quantum yield and light saturation point were decreased gradually, but the dark respiration rate and light compensation point were gradually increased, thus the dyssophotic absorption and conversion efficiency of M. sativa in its photosynthesis were directly affected; ② With the increase of soil water stress, the net photosynthetic rate (P_n) and transpiration rate (T_r) of M. sativa leaves were reduced significantly, but the stomatal conductance (G_s) was decreased at first and then increased, which revealed that the stomatal factors under slight water stress were the main factors resulting in the P_n reduction, and the non-stomatal factors under moderate and severe water stress were the main factors resulting in the P_n reduction; ③ When the photosynthetically active radiation (PAR) value was 1 200 μmol·m^?2·s^-1, the water use efficiency (WUE) of slight water stress was significantly higher than that of full irrigation (P<0.05), indicating that moderate water stress could improve the water use efficiency of M. sativa; ④ There was no significant difference of hay yield between slight water stress and fully irrigation, indicating that high forage yield and water-saving irrigation could be achieved under slight water stress.

Key words:

text-indent:.1pt, Medicago sativa')">"> Medicago sativa, drip irrigation, water use efficiency, soil water stress, photosynthesis, biomass, Yongchang, Gansu

刘军, 齐广平, 康燕霞, 马彦麟, 栗志. 土壤水分胁迫对紫花苜蓿光合特性及其生物量的影响[J]. 干旱区研究, 2019, 36(4): 893-900.

LIU Jun, QI Guang-ping, KANG Yan-xia, MA Yan-lin, LI Zhi.

Effects of Soil Water Stress on Photosynthetic Characteristics and Biomass of Medicago sativa [J]. Arid Zone Research, 2019, 36(4): 893-900.

参考文献 37

[1]	李跃,万里强,李向林,等.水分胁迫对不同紫花苜蓿品种幼苗生长特性和根系ABA含量的影响[J].中国农业大学学报,2017,22(10):75-83.[Li Yue,Wan Liqiang,Li Xianglin,et al.Effects of water stress on the growth characteristics and root ABA content of alfalfa[J].Journal of China Agricultural University,2017,22(10):75-83.]
[2]	李国龙,孙亚卿,张少英.水分胁迫对甜菜幼苗光合作用的影响[J].内蒙古农业大学学报(自然科学版),2012,33(1):68-72.[Li Guolong,Sun Yaqing,Zhang Shaoying.Effects of water stress on photosynthesis of sugar seedling[J].Journal of Inner Mongolia Agricultural University (Natural Science Edition),2012,33(1):68-72.]
[3]	李立辉,王岩,胡海燕,等.初花期干旱对不同抗旱性紫花苜蓿光合特征及荧光参数的影响[J].华北农学报,2015,30(4):126-131.[Li Lihui,Wang Yan,Hu Haiyan,et al.Responses of photosynthetic charaters and fluorescence parameters of alfalfa with different drought tolerances to drought[J].Acta Agriculturae Boreali-Sinica,2015,30(4):126-131.]
[4]	Li S H,Xu X,He J.Study on photosynthetic physiology characteristic of [WTBX]Cynanchum komarovii underwater stress[J].Acta Botanica Boreali Occidentalia Sinica,2004,24(1):100-104.
[5]	范苏鲁,苑兆和,冯立娟,等.水分胁迫对大丽花光合作用、蒸腾和气孔导度的影响[J].中国农学通报,2011,27(8):119-122.[Fan Sulu,Yuan Zhaohe,Feng Lijuan,et al.Effects of water stress on photosynthesis,transpiration and stomatal conductance in dahlia leaves[J].Chinese Agricultural Science Bulletin,2011,27(8):119-122.]
[6]	田德龙,李熙婷,郭克贞,等.河套灌区地下滴灌对紫花苜蓿生长特性的影响[J].节水灌溉,2015(5):16-19.[Tian Delong,Li Xiting,Guo Kezhen,et al.The influence of subsurface drip irrigation to alfalfa growth characteristics in Hetao irrigation area[J].Water Saving Irrigation,2015(5):16-19.]
[7]	郭彦芬,霍轶珍,王文达.紫花苜蓿耗水规律及灌溉制度优化研究[J].节水灌溉,2017(3):8-10,13.[Guo Yanfen,Huo Yizhen,Wang Wenda.A study on water consumption law and irrigation regime for alfalfa[J].Water Saving Irrigation,2017(3):8-10,13.]
[8]	刘文兰,师尚礼,田福平.种植密度对紫花苜蓿生物量与不同叶位光合特性的影响[J].草原与草坪,2017,37(4):14-19,25.[Liu Wenlan,Shi Shangli,Tian Fuping.Effects of planting density on biomass and photosynthetic characteristics of alfalfa leaves at different position[J].Grassland and Turf,2017,37(4):14-19,25.]
[9]	陈托兄,郝文军,陈小兵,等.10个紫花苜蓿品种光合特性的比较[J].中国草地学报,2009,31(2):41-45.[Chen Tuoxiong,Hao Wenjun,Chen Xiaobing,et al.Comparison of photosynthetic characteristics of 10 alfalfa cultivars[J].Chinese Journal of Grassland,2009,31(2):41-45.]
[10]	王冲,王飞,薛韬,等.不同滴灌管埋深对紫花苜蓿水分利用效率和草地覆盖率的影响[J].节水灌溉,2018(1):42-44.[Wang Chong,Wang Fei,Xue Tao,et al.A study on water use efficiency and grassland coverage of alfalfa under different drip pipes buried depth[J].Water Saving Irrigation,2018(1):42-44.]
[11]	闫曼曼,郑剑超,张巨松,等.调亏灌溉对海岛棉光合物质生产与分配的影响[J].干旱区研究,2016,33(6):1 351-1 357.[Yan Manman,Zheng Jianchao,Zhang Jusong,et al.Effects of regulated deficit irrigation on production and distribution of photosynthetic matter in Possypium barbadense L.[J].Arid Zone Research,2016,33(6):1 351-1 357.]
[12]	闫艳红,杨文钰,张新全,等．施氮量对套作大豆花后光合特性、干物质积累及产量的影响[J].草业学报,2011,20(3):233-238.[Yan Yanhong,Yang Wenyu,Zhang Xinquan,et al.Effects of different nitrogen levels on photosynthetic characteristics,dry matter accumulation and yield of relay strip intercropping Gglycine max after blooming[J].Acta Pratacultura Sinica,2011,20(3):233-238.]
[13]	杨司睿,范井伟,孙永强,等.罗布泊腹地人工植被梭梭的光学特性及其对干旱胁迫的响应[J].干旱区研究,2018,35(2):379-386.[Yang Sirui,Fan Jingwei,Sun Yongqiang,et al.Photosynthetic characteristics and response of Haloxylon ammodendron to drought stress in hinterland of the Lop Nur[J].Arid Zone Research,2018,35(2):379-386.]
[14]	郑睿,康绍忠,胡笑涛,等.水氮处理对荒漠绿洲区酿酒葡萄光合特性与产量的影响[J].农业工程学报,2013,29(4):133-141.[Zheng Rui,Kang Shaozhong,Hu Xiaotao,et al.Effects of water and nitrogen conditions on the diurnal variation of photosynthesis characteristics and yield of grapevine in arid oasis region[J].Transations of the Chinese Society of Agricultural Engineering,2013,29(4):133-141.]
[15]	于文颖,纪瑞鹏,冯锐,等.不同生育期玉米叶片光合特性及水分利用效率对水分胁迫的响应[J].生态学报,2015,35(9):2 902-2 909.[Yu Wenying,Ji Ruipeng,Feng Rui,et al.Responses of water stress on photosynthetic characteristics and water use efficiency of maize leaves in different growth stages[J].Acta Ecologica Sinica,2015,35(9):2 902-2 909.]
[16]	冯晓敏,张永清.水分胁迫对糜子植株苗期生长和光合特性的影响[J].作物学报,2012,38(8):1 513-1 521.[Feng Xiaomin,Zhang Yongqing.Effects of water stress on seedling growth and photosynthetic characteristics in broomcorn millet[J].Acta Agronomica Sinica,2012,38(8):1 513-1 521.]
[17]	韩瑞宏,卢欣石,高桂娟,等.紫花苜蓿 (Medicago sativa)对干旱胁迫的光合生理响应[J].生态学报,2007(12):5 229-5 237.[Han Ruihong,Lu Xinshi,Gao Guijuan,et al.Photosynthetic physiological response of alfalfa (Medicago sativa) to drought stress[J].Acta Ecologica Sinica,2007(12):5 229-5 237.]
[18]	张前兵,李艳霞,于磊,等.5个苜蓿品种群体光合生产特征比较[J].西北农业学报,2017,26(6):873-881.[Zhang Qianbing,Li Yanxia,Yu Lei,et al.Canopy apparent photosynthetic characteristics of five alfalfa varieties[J].Acta Agricultural Boreali Occidentalis Sinica,2017,26(6):873-881.]
[19]	温方.紫花苜蓿不同品种生产性能及其光合特性研究[D].北京：中国农业科学院,2007.[Wen Fang.Study on Productivity and Photosynthetic Characteristics of Different Alfalfa Varieties[D].Beijing：Chinese Academy of Agricultural Sciences,2007.]
[20]	李艳芳.12个国外引进苜蓿品种光合蒸腾特性的研究[D].杨凌：西北农林科技大学,2011.[Li Yanfang.Study on Photosynthetic and Transpiration Characteristics of 12 Introduced Alfalfa Varieties[D].Yangling：Northwest A&F University,2011.]
[21]	刘国利,何树斌,杨惠敏.紫花苜蓿水分利用效率对水分胁迫的响应及其机理[J].草业学报,2009,18(3):207-213.[Liu Guoli,He Shubin,Yang Huimin.The response and mechanism of water use efficiency to different water stress of three alfalfa varieties[J].Acta Pratacultura Sinica,2009,18(3):207-213.]
[22]	叶子飘,于强.光合作用光响应模型的比较[J].植物生态学报，2008,32(6):1 356-1 361.[Ye Zipiao,Yu Qiang.Comparison of new and several classical models of photo-synthesis in response to irradiance[J].Chinese Journal of plant Ecology,2008,32(6):1 356-1 361.]
[23]	陆燕元,马焕成,李昊民,等.土壤干旱对转基因甘薯光合曲线的响应[J].生态学报,2015,35(7):2 155-2 160.[Lu Yanyuan,Ma Huancheng,Li Haomin,et al.Light response characteristics of phpotosynthetic of transgenic sweet potato under drought stress[J].Acta Ecologica Sinica,2015,35(7):2 155-2 160.]
[24]	Xia J B,Zhang S Y,Zhang G C,et al.Critical response of photosynthetic efficiency in Campsis radicans (L.) seem to soil water and light intensities[J].African journal of Biotechnology,2011,10(77):17 748-17 754.]
[25]	倪霞,曹永慧,周本智,等.干旱处理对毛竹光响应的影响：基于４种模型比较分析[J].林业科学研究,2017,30(3):465-471.[Ni Xia,Cao Yonghui,Zhou Benzhi,et al.Light response of Phyllostachys edulis under drought stress:Based on 4 models[J].Forestry Research,2017,30(3):465-471.]
[26]	牟兰,何承刚,姜华,等.干热胁迫对紫花苜蓿光合特性的影响[J].草地学报,2014,22(3):550-555.[Mou Lan,He Chenggang,Jiang Hua,et al.Effect of drought and heatstress on photosynthetic characteristics of alfalfa[J].Acta Agrestia Sinica,2014,22(3):550-555.]
[27]	高冠龙,冯起,张小由,等.植物叶片光合作用的气孔与非气孔限制研究综述[J].干旱区研究,2018,35(4):929-937.[Gao Guanlong,Feng Qi,Zhang Xiaoyou,et al.An overview of stomatal and non-stomatal limitations to photosynthesis of plants[J].Arid Zone Research,2018,35(4):929-937.]
[28]	Farquhar G D,Sharkey T D.Stomatal conductance and photosynthesis[J].Annual Review of Plant Physiology,1982,33(1):317-345.
[29]	韩清芳,贾志宽,王俊鹏,等.黄土高原地区紫花苜蓿不同叶位光合日变化特征研究[J].草地学报,2009,17(5):558-563.[Han Qingfang,Jia Zhikuan,Wang Junpeng,et al.Study on diurnal photosynthesis characteristics in alfalfa leaf layers in Loess Plateau[J].Acta Academia Sinica,2009,17(5):558-563.]
[30]	李志军,罗青红,伍维模,等.干旱胁迫对胡杨和灰叶胡杨光合作用及叶绿素荧光特性的影响[J].干旱区研究,2009,26(1):45-52.[Li Zhijun,Luo Qinghong,Wu Weimo,et al.The effects of drought stress on photosynthesis and chlorophyll fluorescence characteristics of Populus euphratica and P.euphratica [J].Arid Zone Research,2009,26(1):45-52.]
[31]	Lakso A N.Seasonal changes in stomatal responses to leaf water potential in apple[J].Journal American Society for Horticultural Science,1979,104(1):58-60.
[32]	Yang H M,Zhang X Y,Wang G X.Relationship between stomatal character,photosynthetic character and seed chemical composition in grass pea at different water availabilities[J].Journal of Agricultural Science,2004,142:675-681.
[33]	韩希英,宋凤斌,王波,等.土壤水分胁迫对玉米光合特性的影响[J].华北农学报,2006,21(5):28-32.[Han Xiying,Song Fengbin,Wang Bo,et al.Effects of soil water stress on photosynthetic characteristics of maize[J].Acta Agriculturae Boreali-Sinica,2006,21(5):28-32.]
[34]	杨涛,梁宗锁,薛吉全.土壤干旱不同玉米品种水分利用效率差异的生理学原因[J].干旱地区农业研究,2002,20(2):68-71.[Yang Tao,Liang Zongsuo,Xue Jiquan.Physiological mechanism of diversity of water use efficiency of maize varieties in soil drought[J].Agricultural Research in the Arid Areas,2002,20(2):68-71.]
[35]	曹雪松,李和平,郑和祥,等.地埋滴灌对紫花苜蓿耗水、产量及水分生产率的影响[J].干旱地区农业研究,2017,35(5):256-262.[Cao Xuesong,Li Heping,Zheng Hexiang,et al.Effects of subsurface drip irrigation on water consumption,yield and water productivity of alfalfa[J].Agricultural Research in the Arid Areas,2017,35(5):256-262.]
[36]	郭松年,丁林,王福霞.作物调亏灌溉理论与技术研究进展及发展趋势[J].中国农村水利水电,2009(8):12-16.[Guo Songnian,Ding Lin,Wang Fuxia.Theoretical and technology research advancement and development trend prospect of regulated deficit irrigation on crops[J].China Rural Water and Hydropower,2009(8):12-16.]
[37]	陈林,王磊,宋乃平,等.灌溉量和灌溉次数对紫花苜蓿耗水特性和生物量的影响[J].水土保持学报,2009,23(4):91-95.[Chen Lin,Wang Lei,Song Naiping,et al.Effects of irrigation amount and times on water consumption characteristics and biomass of alfalfa[J].Journal of Soil and Water Conservation,2009,23(4):91-95.]