新疆伊犁野生阿魏菇根际土壤环境因子与细菌群落组成特征

doi:10.13866/j.azr.2025.05.10

Abstract

Abstract:

In order to reveal the characteristics of rhizosphere soil bacterial communities of wild, Pleurotus ferulae in Ili region, Xinjiang, China and their interaction mechanisms with soil environmental factors, and to break through the bottlenecks of artificial domestication, this study integrated soil physicochemical analysis, soil enzyme activity assays, and Illumina high-throughput sequencing to compare the microecological differences between rhizosphere and non-rhizosphere soils. The results revealed that the pH of rhizosphere soil of P. ferulae was significantly lower than that of non-rhizosphere soil, and organic matter, available nitrogen/phosphorus, and enzyme activities were markedly higher than those of non-rhizosphere soil, indicating a synergistic regulation of nutrient cycling by the host and associated microorganisms. Sequencing identified 1895 bacterial Operational Taxonomic Units (OTUs), with 156 unique to the rhizosphere and 102 unique to non-rhizosphere soils. Bacteroidetes (14.26%), Gemmatimonadetes (4.87%), and Verrucomicrobia (1.24%) were significantly enriched in the rhizosphere soil. Specific genera such as Pseudomonas and Flavobacterium may support the growth of P. ferulae through organic matter degradation and plant growth-promoting functions. Redundancy Analysis (RDA) revealed that organic matter, available nitrogen and phosphorus, and β-Glucosidase were key factors driving community structure (cumulative explanation >70%). Bacteroidetes and Acidobacteria were linked to carbon and nitrogen cycling, whereas Proteobacteria and Chloroflexi were associated with pH adaptability. In artificial cultivation, it is recommended to simulate a near-neutral pH environment in rhizosphere soil, enhance organic matter supplementation, and inoculate functional microbiota to optimize mycelial colonization efficiency. These results provide a theoretical basis for the conservation and sustainable utilization of P. ferulae.

Key words: wild Pleurotus ferulae, high-throughput sequencing, rhizosphere soil, environmental factor, bacterial community

WANG Guangquan, Muguli MUHAXI, Oren AKHBERDI, Mayira TURDIBEK, ZHANG Xuemei, PANG Kejian. Characterization of environmental factors and bacterial community composition in rhizosphere soil of wild Pleurotus ferulae in Xinjiang Ili[J].Arid Zone Research, 2025, 42(5): 875-884.

Figures/Tables 8

Tab. 1

Tab. 2

Tab. 3

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Fig. 5

References 37

[1]	Fuhrman J A. Microbial community structure and its functional implications[J]. Nature, 2009, 459(7244): 193-199.
[2]	Naeem S, Li S. Biodiversity enhances ecosystem reliability[J]. Nature, 1997, 390(6659): 507-509.
[3]	Shi S, Nuccio E, Herman D J, et al. Successional trajectories of rhizosphere bacterial communities over consecutive seasons[J]. MBio, 2015, 6(4): 1-8.
[4]	余炎炎, 李梦莎, 刘啸林, 等. 大兴安岭典型永久冻土土壤细菌群落组成和多样性[J]. 微生物学通报, 2020, 47(9): 2759-2770.
	[Yu Yanyan, Li Mengsha, Liu Xiaolin, et al. Composition and diversity of soil bacterial communities in typical permafrost regions of the Greater Khingan Mountains[J]. Microbiology China, 2020, 47(9): 2759-2770.]
[5]	王晓波. 我国北方草地土壤微生物群落的空间格局及其驱动机制[D]. 沈阳: 中国科学院沈阳应用生态研究所, 2016.
	[Wang Xiaobo. Spatial Patterns and Driving Mechanisms of Soil Microbial Communities in Grasslands of Northern China[D]. Shenyang: Shenyang Institute of Applied Ecology, Chinese Academy of Sciences, 2016.]
[6]	黄俊杰, 陆雅海. 土壤拟杆菌与梭菌分解多糖类有机物质的研究进展与展望[J]. 微生物学通报, 2022, 49(3): 1147-1157.
	[Huang Junjie, Lu Yahai. Research progress and prospects on the decomposition of polysaccharides by soil Bacteroidetes and Clostridia[J]. Microbiology China, 2022, 49(3): 1147-1157.]
[7]	斯林林, 徐静, 曹凯, 等. 绿肥种植对红壤旱地生土细菌群落结构的影响[J]. 浙江农业学报, 2023, 35(8): 1864-1875. doi: 10.3969/j.issn.1004-1524.20221096
	[Si Linlin, Xu Jing, Cao Kai, et al. Effects of green manure cultivation on bacterial community structure in raw soil of red soil dryland[J]. Acta Agriculturae Zhejiangensis, 2023, 35(8): 1864-1875.] doi: 10.3969/j.issn.1004-1524.20221096
[8]	Jones R T, Robeson M S, Lauber C L, et al. A comprehensive survey of soil acidobacterial diversity using pyrosequencing and clone library analyses[J]. The ISME Journal Emultidisciplinary Journal of Microbial Ecology, 2009, 3(4): 442-453.
[9]	秦杰, 姜昕, 周晶, 等. 长期不同施肥黑土细菌和古菌群落结构及主效影响因子分析[J]. 植物营养与肥料学报, 2015, 21(6): 1590-1598.
	[Qin Jie, Jiang Xin, Zhou Jing, et al. Analysis of bacterial and archaeal community structure and main influencing factors in black soil under long-term different fertilization practices[J]. Journal of Plant Nutrition and Fertilizers, 2015, 21(6): 1590-1598.]
[10]	Janssen P H. Identifying the dominant soil bacterial taxa in libraries of 16S rRNA and 16S rRNA genes[J]. Applied and Environmental Microbiology, 2006, 72(3): 1719-1728. doi: 10.1128/AEM.72.3.1719-1728.2006 pmid: 16517615
[11]	Ranjard L, Poly F, Nazaret S. Monitoring complex bacterial communities using culture-independent molecular techniques: Application to soil environment[J]. Research in Microbiology, 2000, 151(3): 167-177. doi: 10.1016/s0923-2508(00)00136-4 pmid: 10865943
[12]	武华周, 娄德钊, 涂娜娜, 等. 抗感青枯病桑树根际细菌群落结构与多样性[J]. 福建农业学报, 2020, 35(9): 1004-1011.
	[Wu Huazhou, Lou Dezhao, Tu Nana, et al. Structure and diversity of bacterial communities in the rhizosphere of mulberry trees resistant to bacterial wilt disease[J]. Fujian Journal of Agricultural Sciences, 2020, 35(9): 1004-1011.]
[13]	木古丽·木哈西, 博涛, 吾尔恩·阿合别尔迪, 等. 新疆伊犁野生阿魏菇的生长发育规律及生境分析[J]. 干旱区资源与环境, 2021, 35(4): 160-164.
	[Muhaxi Muguli, Bo Tao, Ahebierdi Wueren, et al. Growth and development patterns and habitat analysis of wild Pleurotus ferulae in Yili, Xinjiang[J]. Journal of Arid Land Resources and Environment, 2021, 35(4): 160-164.]
[14]	Liu M H, Liu F, Ng T B, et al. Purification and characterization of pleuroferin, a novel protein with in vitro anti-non-small cell lung cancer activity from the mushroom Pleurotus ferulae lanzi[J]. Process Biochemistry, 2022, 122: 16-25.
[15]	Muguli M, Liu F, Ng T B. Structural characterization and in vitro hepatoprotective activity of a novel antioxidant polysaccharide from fruiting bodies of the mushroom Pleurotus ferulae[J]. International Journal of Biological Macromolecules, 2023, 243: 125124.
[16]	木古丽·木哈西, 吾尔恩·阿合别尔迪, 刘方, 等. 新疆伊犁野生阿魏菇菌株的分离鉴定与培养特性[J]. 微生物学杂志, 2021, 41(6): 103-110.
	[Muhaxi Muguli, Ahebierdi Wueren, Liu Fang. Isolation, identification, and cultural characteristics of wild Pleurotus ferulae strains from Yili, Xinjiang[J]. Journal of Microbiology, 2021, 41(6): 103-110.]
[17]	郑剑英, 李宇辉, 张晓荣, 等. 土壤养分速测的土样采集方法[J]. 吉林农业, 2006(2): 23.
	[Zheng Jianying, Li Yuhui, Zhang Xiaorong, et al. Soil sampling methods for rapid soil nutrient testing[J]. Agriculture of Jilin, 2006(2): 23.]
[18]	鲍士旦. 土壤农化分析[M]. 北京: 中国农业出版社, 2000.
	[Bao Shidan. Soil Agro-Chemical Analysis[M]. Beijing: China Agriculture Press, 2000.]
[19]	冯慧琳, 徐辰生, 何欢辉, 等. 生物炭对土壤酶活和细菌群落的影响及其作用机制[J]. 环境科学, 2021, 42(1): 422-432.
	[Feng Huilin, Xu Chensheng, He Huanhui, et al. Effects of biochar on soil enzyme activity and bacterial community and its mechanisms[J]. Environmental Science, 2021, 42(1): 422-432.]
[20]	张艳, 郭书亚, 尚赏, 等. 甘薯/玉米不同间作方式对土壤养分、酶活性及作物产量的影响[J]. 山西农业科学, 2020, 48(8): 1234-1238.
	[Zhang Yan, Guo Shuya, Shang Shang, et al. Effects of different sweet potato/maize intercropping patterns on soil nutrients, enzyme activity, and crop yield[J]. Journal of Shanxi Agricultural Sciences, 2020, 48(8): 1234-1238.]
[21]	Mori H, Maruyama F, Kato H, et al. Design and experimental application of a novel non-degenerate universal primer set that amplifies prokaryotic 16S rRNA genes with a low possibility to amplify eukaryotic rRNA genes[J]. DNA Research, 2014, 21(2): 217-227. doi: 10.1093/dnares/dst052 pmid: 24277737
[22]	Xu N, Tan G C, Wang H Y, et al. Effect of biochar additions to soil on nitrogen leaching, microbial biomass and bacterial community structure[J]. European Journal of Soil Biology, 2016, 74: 1-8.
[23]	Chen S F, Zhou Y Q, Chen Y R, et al. Fastp: An ultra-fast all-in-one FASTQ preprocessor[J]. Bioinformatics, 2018, 34(17): 884-890. doi: 10.1093/bioinformatics/bty560 pmid: 30423086
[24]	Magoč T, Salzberg S L. FLASH: Fast length adjustment of short reads to improve genome assemblies[J]. Bioinformatics, 2011, 27(21): 2957-2963. doi: 10.1093/bioinformatics/btr507 pmid: 21903629
[25]	Edgar R C. UPARSE: Highly accurate OTU sequences from microbial amplicon reads[J]. Nat Methods, 2013, 10(10): 996-998. doi: 10.1038/nmeth.2604 pmid: 23955772
[26]	Stackebrandt E, Goebel B M. Taxonomic note: A place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology[J]. International Journal of Systematic Bacteriology, 1994, 44(4): 846-849.
[27]	Wang Q, Garrity G M, Tiedje J M, et al. Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy[J]. Applied and Environmental Microbiology, 2007, 73(16): 5261-5267. doi: 10.1128/AEM.00062-07 pmid: 17586664
[28]	Langille M G I, Zaneveld J, Caporaso J G, et al. Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences[J]. Nature Biotechnology, 2013, 31(9): 814-821. doi: 10.1038/nbt.2676 pmid: 23975157
[29]	王宏伟, 黄清俊, 李萍, 等. 3种草本植物盐碱土栽培地的根际环境变化[J]. 上海农业学报, 2012, 28(3): 66-69.
	[Wang Hongwei, Huang Qingjun, Li Ping, et al. The rhizosphere soil environment changes after growing 3 species of herbaceous plants on saline alkali soil[J]. Acta Agriculturae Shanghai, 2012, 28(3): 66-69.]
[30]	Guo Xiaolin, Zhou Yongbin. Effects of land use patterns on the bacterial community structure and diversity of wetland soils in the Sanjiang Plain[J]. Journal of Soil Science and Plant Nutrition, 2021, 21(1): 1-12.
[31]	文少白, 李勤奋, 侯宪文, 等. 微生物降解纤维素的研究概况[J]. 中国农学通报, 2010, 26(1): 231-236.
	[Wen Shaobai, Li Qinfen, Hou Xianwen, et al. Recent advances in microbial degradation of cellulose[J]. Chinese Agricultural Science Bulletin, 2010, 26(1): 231-236.] doi: 10.11924/j.issn.1000-6850.2009-1564
[32]	Cao T T, Luo Y C, Shi M, et al. Microbial interactions for nutrient acquisition in soil: Miners, scavengers, and carriers[J]. Soil Biology and Biochemistry, 2024, 188: 109215.
[33]	赵亚楠. 荒漠草原灌丛人为转变过程中土壤碳氮耦合特征及机制[D]. 银川: 宁夏大学, 2022.
	[Zhao Yanan. Soil Carbon and Nitrogen Coupling Characteristics and Mechanisms During Anthropogenic Transformation of Desert Steppe Shrub[D]. Yinchuan: Ningxia University, 2022.]
[34]	Zhang H, Sekiguchi Y, Hanada S. Gemmatimonas aurantiaca gen. nov. sp. nov. a gram-negative, aerobic, polyphosphate-accumulating micro-organism, the first cultured representative of the new bacterial phylum Gemmatimonadetes phyl. nov.[J]. International Journal of Systematic and Evolutionary Microbiology, 2003, 53(4): 1155-1163.
[35]	PLugtenberg B, Kamilova F. Plant-growth-promoting rhizobacteria[J]. Annual Review of Microbiology, 2009, 63: 541-556. doi: 10.1146/annurev.micro.62.081307.162918 pmid: 19575558
[36]	Trivedi P, Anderson I C, Singh B K. Microbial modulators of soil carbon storage: Integrating genomic and metabolic knowledge for global prediction[J]. Trends in Microbiology, 2013, 21(12): 641-651. doi: 10.1016/j.tim.2013.09.005 pmid: 24139848
[37]	肖德顺, 徐春梅, 王丹英, 等. 增氧模式对水稻根际微生物多样性和群落结构的影响[J]. 环境科学, 2023, 44(11): 6362-6376.
	[Xiao Deshun, Xu Chunmei, Wang Danying, et al. Effects of oxygen enhancement mode on rhizosphere microbial diversity and community structure of rice[J]. Environmental Science, 2023, 44(11): 6362-6376.]

土壤类型	生境特征	样本编号	经纬度	海拔/m	土壤取样深度/cm
根际土壤	保护区内，3 a生以上新疆阿魏植株根茎部土壤（设为I_A）	I_A1	43°44′49″N，82°07′27″E	1092	10~20
		I_A2	43°44′48″N，82°07′32″E	1192	10~20
		I_A3	43°44′48″N，82°07′37″E	1191	10~20
非根际土壤	保护区外，无新疆阿魏和阿魏菇生长的土壤（设为C）	C1	43°44′50″N，82°06′38″E	1090	10~20
		C2	43°44′23″N，82°06′46″E	1092	10~20
		C3	43°44′54″N，82°07′00″E	1089	10~20

土壤样品	I_A1	I_A2	I_A3	C1	C2	C3
土壤样本来源	保护区内	保护区内	保护区内	保护区外	保护区外	保护区外
土壤样本来源	根际土壤	根际土壤	根际土壤	非根际土壤	非根际土壤	非根际土壤
pH	7.87±0.14b	8.12±0.16b	7.96±0.04b	8.51±0.14a	8.68±0.21a	8.77±0.09a
MC/%	8.92±0.04b	8.49±0.03c	11.64±0.06a	1.16±0.02d	1.28±0.06d	1.24±0.003d
OM/（g·kg^-1）	36.9±0.07a	42.23±3.99a	44.23±4.99a	20.28±2.55b	16.64±1.67b	19.22±1.84b
AN/（mg·kg^-1）	46.23±0.07a	35.32±1.78b	34.55±1.72b	31.16±0.27c	27.28±1.06d	32.24±1.29bc
AP/（mg·kg^-1）	3.87±0.06a	3.59±0.19b	4.26±0.40a	3.01±0.04c	2.81±0.70c	3.45±0.22b
AK/（mg·kg^-1）	163.58±3.65b	176.23±6.61a	136.21±5.35d	175.86±5.74a	180.11±1.67a	156.61±3.04c
POD/（U·g^-1）	108.82±4.49b	114.60±4.88b	132.96±7.87a	107.89±3.61b	100.12±2.58c	98.66±5.00c
UE/（U·g^-1）	898.45±11.04b	915.22±12.24b	937.91±2.98a	747.58±46.78c	700.11±0.57c	658.62±3.86d
β-GC/（U·g^-1）	37.59±3.87cd	67.31±4.16a	55.29±3.31b	45.48±3.07c	26.17±1.97d	30.65±1.55d

土壤类型	Sobs 指数	Chao 指数	Simpson 指数	Shannon 指数	文库覆盖度指数/%
I_A	1339±111.88a	1495.1±111.88a	0.011646±0.004771a	5.7692±0.19297a	0.9950
C	1462±64.09a	1597.6±111.88a	0.007126±0.000549a	6.0022±0.06794a	0.9949

Characterization of environmental factors and bacterial community composition in rhizosphere soil of wild Pleurotus ferulae in Xinjiang Ili

RichHTML

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 8

References 37

Related Articles 15

Metrics

Comments

Recommended 0

[1]	XU Wentao, DU Yongjun, ZHANG Heng, TIAN Hao, CHAI Wenguang, LI Xiaolong, JIA Weikang, YANG Guang. Characteristics and factors influencing water and heat flux changes in typical desert ecosystems in arid inland river basins [J]. Arid Zone Research, 2025, 42(9): 1574-1586.
[2]	JIANG Kangwei, WANG Yafei, LIU Chentong, LI Hong, LYU Cheng, Tursunnay REYIMU, ZHANG Qingqing. Responses of soil microbial communities to grazing and their relationship with environmental factors [J]. Arid Zone Research, 2025, 42(3): 467-479.
[3]	YANG Ying, LI Qin, WANG Yuan, YANG Guijun, TIAN Jinhua, LI Xiong, ZHANG Dazhi. Bird community diversity and influencing factors in the Taiyangshan wind farm of Ningxia [J]. Arid Zone Research, 2025, 42(10): 1899-1912.
[4]	CHEN Songqing, DONG Hongfang, YUE Yifeng, HAO Yuanyuan, LIU Xin, CAO Xianyu, MA Jun. Geographical distribution and dynamic change prediction of Hippophae rhamnoides subsp. sinensis under different climate scenarios [J]. Arid Zone Research, 2024, 41(9): 1560-1571.
[5]	ZHAO Lichao, ZHANG Chengfu, HE Shuai, MIAO Lin, FENG Shuang, PAN Sihan. Simulation of land surface temperature in complex mountainous terrain and the influence of environmental factors: A case study in Daqingshan, Inner Mongolia [J]. Arid Zone Research, 2024, 41(5): 765-775.
[6]	AN Ning, GUO Bin, ZHANG Dongmei, YANG Qiyue, LUO Weicheng. Desert vegetation composition and spatial distribution of soil nutrients in the middle section of Hexi Corridor [J]. Arid Zone Research, 2024, 41(3): 432-443.
[7]	CHAI Qiaodi, MA Rui, WANG Anlin, ZHANG Fu, LIU Teng, TIAN Yongsheng. Leaf functional traits of typical desert plants in the sand-blocking and sand-fixing belt of the Hexi Corridor [J]. Arid Zone Research, 2024, 41(11): 1898-1907.
[8]	LI Juan, LIU Yang, LIU Guangxiu, CHENG Liang, GUO Qingyun, ZHANG Wei, ZHANG Gaosen. Study on bacterial community structure and influencing factors in the northern margin of the Shanshan Kumtag Desert [J]. Arid Zone Research, 2023, 40(8): 1358-1368.
[9]	LI Jiannan, SHI Haibin, MIAO Qingfeng, SHAN Dan, RONG Hao, WEN Yaqin. Effect of environmental factors on the transpiration water consumption of various artificial arbor stands [J]. Arid Zone Research, 2023, 40(8): 1312-1321.
[10]	LI Rui, SHAN Lishan, XIE Tingting, MA Li, YANG Jie, LI Quangang. Variation in the leaf functional traits of typical desert shrubs under precipitation gradient [J]. Arid Zone Research, 2023, 40(3): 425-435.
[11]	WU Rui, CAO Hongyu, GAO Guanglei, YU Minghan, DING Guodong, ZHANG Ying, ZHAO Peishan. Effects of irrigation and salinity treatments on the soil bacterial community and plant physiological characteristics of Cyperus esculentus farmland in Horqin Sandy Land [J]. Arid Zone Research, 2023, 40(12): 1938-1948.
[12]	YAN Peiying,QU Jianjun,WANG Lide,XIAO Jianhua,ZHANG Yuan,WANG Xiaohong,GUO Shujiang. Effect of sand barrier fixation on the formation and development of biological soil crust [J]. Arid Zone Research, 2023, 40(12): 1931-1937.
[13]	WANG Guanzheng, CHANG Shunli, WANG Jianping, ZHANG Yutao, SUN Xuejiao, LI Xiang. Factors affecting Picea schrenkiana regeneration on different slope directions [J]. Arid Zone Research, 2023, 40(10): 1661-1669.
[14]	XU Jieliang,ZHANG Fenghua,LI Bianbian,WANG Jiaping,CHENG Zhibo. Effect of fertilization on the characteristics of soil microbial community in the rhizosphere of Cyperus esculentus in the sandy area of Xinjiang [J]. Arid Zone Research, 2021, 38(6): 1741-1749.
[15]	YAN Jingming,ZHOU Xiaobing,ZHANG Jing,TAO Ye. Variation in one-year-old branch stoichiometry of Malus sieversii at different altitudes and the influencing factors in Tianshan Mountains, China [J]. Arid Zone Research, 2021, 38(2): 450-459.