梭梭种子内生微生物群落组成及功能

doi:10.13866/j.azr.2025.09.08

Abstract

Abstract:

This study aimed to reveal the ecological adaptive mechanisms and agricultural potential of endophytic microorganisms in desert plant seeds, using Haloxylon ammodendron seeds as the research subject. We combined high-throughput sequencing and conventional culture techniques to analyze the structural characteristics of endophytic microbial communities in H. ammodendron seeds and elucidate their potential roles in plant growth promotion and stress resistance. The results revealed that the endophytic bacterial community in H. ammodendron seeds comprised 668 species spanning 31 phyla, dominated by Firmicutes and Bacteroidetes, whereas the endophytic fungal community included 583 species spanning 13 phyla, dominated by Ascomycota and Basidiomycota. Functional annotation revealed distinct metabolic roles: the endophytic bacteria were enriched in chemoheterotrophy and fermentation, whereas the fungal communities exhibited saprophytic and pathogenic traits. Using conventional culture methods, we isolated 13 culturable endophytic bacterial strains, including two multifunctional plant growth-promoting strains (Priestia aryabhattai HB-4 and Priestia megaterium HB-9) and three salt-tolerant strains (Bacillus zhangzhouensis HB-6, Bacillus safensis HB-10, and Bacillus pumilus HB-11). Pot experiments demonstrated that HB-4 and HB-9 significantly enhanced wheat growth, while HB-6, HB-10, and HB-11 alleviated saline-alkaline stress in wheat. This study elucidates the ecological roles of endophytic microbes in H. ammodendron seeds, providing novel insights into the exploitation of microbial resources in desert ecosystems and the development of plant growth-promoting microbial agents.

Key words: Haloxylon ammodendron seeds, endophytic microorganisms, community structure, diversity, growth-promoting bacterial

ZHU Zhaohua, ZHAI Yixiao, LI Xinrong, Miao Yingxiang, MA Tong, LI Shanjia. Community composition and functionalities of endophytic microorganisms in Haloxylon ammodendron seeds[J].Arid Zone Research, 2025, 42(9): 1640-1649.

Figures/Tables 11

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References 35

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分组	土壤处理	浇水处理
对照（CK）	无菌水浸透土壤	定期浇无菌水
盐胁迫组	150 mmol·L^-1NaCl溶液浸透土壤	定期浇无菌水
碱胁迫组	150 mmol·L^-1无菌NaHCO₃溶液浸透土壤	定期浇无菌水
盐胁迫+菌剂处理组	150 mmol·L^-1NaCl溶液浸透土壤	定期浇菌悬液
碱胁迫+菌剂处理组	150 mmol·L^-1无菌NaHCO₃溶液浸透土壤	定期浇菌悬液

指数	ACE	Chao1	Simpson	Shannon	Coverage
细菌	1647.45±29.79	1638.44±28.75	0.98±0.00	7.78±0.02	1.00
真菌	593.11±126.47	590.84±125.92	0.98±0.00	7.36±0.19	1.00

菌株	比对结果	相似度/%	NCBI序列号	GenBank登录号
HB-1	高空芽孢杆菌Bacillus altitudinis	99.88	NR_042337.1	PQ774797
HB-2	短小芽孢杆菌Bacillus pumilus	98.94	NR_115334.1	PQ774798
HB-3	空气芽孢杆菌Bacillus aerius	99.88	NR_118439.1	PQ836096
HB-4	阿氏普里斯特氏菌Priestia aryabhattai	100.00	NR_115953.1	PQ774800
HB-5	玫瑰色考克氏菌Kocuria rosea	99.89	NR_044871.1	PQ836097
HB-6	漳州芽孢杆菌Bacillus zhangzhouensis	99.89	NR_148786.1	PQ836098
HB-7	耐寒短杆菌Peribacillus frigoritolerans	98.25	NR_115064.1	PQ774803
HB-8	巨大芽孢杆菌Priestia megaterium	97.54	NR_116873.1	PQ836099
HB-9	巨大芽孢杆菌Priestia megaterium	98.70	NR_112636.1	PQ774805
HB-10	沙福芽孢杆菌Bacillus safensis	97.21	NR_041794.1	PQ836100
HB-11	短小芽孢杆菌Bacillus pumilus	98.58	NR_043242.1	PQ836101
HB-12	地衣芽孢杆菌Bacillus licheniformis	99.78	NR_074923.1	PQ774804
HB-13	嗜糖土地芽孢杆菌Terribacillus aidingensis	97.90	NR_114288.1	PQ836102

菌株	IAA产量/（μg·mL^-1）	固氮	解磷（D/d）	产铁载体	耐盐	耐碱
HB-1	－	+	1.50±0.04ef	－	－	+
HB-2	－	+	1.54±0.09de	－	－	+
HB-3	－	+	2.36±0.13a	－	－	+
HB-4	18.48±2.70a	+	－	－	－	－
HB-5	10.67±0.26b	+	－	－	－	+
HB-6	－	+	1.77±0.05c	－	+	+
HB-7	0.25±0.16d	+	－	+	－	－
HB-8	5.22±2.14c	－	1.40±0.03f	+	－	－
HB-9	8.43±1.07b	－	1.90±0.07b	+	－	－
HB-10	－	+	－	－	+	+
HB-11	－	+	－	－	+	+
HB-12	－	+	1.64±0.01d	－	+	－
HB-13	－	－	－	－	+	－

指标	对照组CK	HB-4处理组	HB-9处理组
鲜重/g	0.16±0.00c	0.36±0.01b	0.43±0.01a
干重/g	0.04±0.00b	0.07±0.01a	0.05±0.00b
茎长/cm	22.83±4.01b	29.50±1.73a	28.50±0.50ab
根长/cm	10.67±1.53b	13.83±1.89ab	15.50±1.32a
叶绿素含量/(mg·g^-1)	1.09±0.09b	1.48±0.13a	1.42±0.20ab
丙二醛含量/(μmol·L^-1)	10.33±0.16a	7.61±0.45b	6.86±1.84b
CAT活性/(U·mg^-1)	2.52±0.05c	4.99±0.07a	3.58±0.19b
POD活性/(U·mg^-1)	73.75±3.61b	92.40±4.66ab	101.64±19.78a
脯氨酸含量/(μg·g^-1)	16.00±0.79b	20.80±1.95a	13.84±1.98b

Community composition and functionalities of endophytic microorganisms in Haloxylon ammodendron seeds

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指标	盐胁迫组	对照组	HB-6处理组	HB-10处理组	HB-11处理组
鲜重/g	0.18±0.03c	0.23±0.01b	0.33±0.02a	0.29±0.01a	0.21±0.01bc
干重/g	0.04±0.01c	0.08±0.01ab	0.08±0.00ab	0.08±0.00a	0.06±0.01b
茎长/cm	26.17±1.89a	28.73±1.08a	30.00±0.50a	29.00±2.29a	29.23±2.36a
根长/cm	10.97±1.38c	20.63±0.51a	19.17±1.61a	19.80±0.30a	14.17±0.76b
叶绿素含量/(mg·g^-1)	0.94±0.03c	1.34±0.02a	1.14±0.05b	1.33±0.09a	1.11±0.07b
丙二醛含量/(μmol·L^-1)	7.79±0.48ab	6.27±0.62c	9.10±0.68a	6.98±0.46bc	7.37±0.64bc
CAT活性/(U·mg^-1)	1.67±0.03b	2.77±0.44a	2.13±0.17ab	2.35±0.41ab	2.54±0.25a
POD活性/(U·mg^-1)	52.57±5.90c	59.76±3.22bc	72.08±6.00ab	77.51±2.74a	73.66±9.38ab
脯氨酸含量/(μg·g^-1)	9.68±0.46c	16.26±0.12a	13.88±1.25b	14.60±0.53b	13.38±0.29b

指标	碱胁迫组	对照组CK	HB-6处理组	HB-10处理组	HB-11处理组
鲜重/g	0.16±0.01c	0.26±0.02b	0.26±0.01b	0.25±0.02b	0.32±0.02a
干重/g	0.05±0.00b	0.06±0.01ab	0.06±0.00ab	0.05±0.00ab	0.07±0.01a
茎长/cm	25.33±0.25a	26.67±1.89a	28.53±0.25a	24.90±3.42a	30.20±2.96a
根长/cm	20.50±1.00a	18.43±2.15a	18.27±2.87a	22.10±0.87a	20.80±1.49a
叶绿素含量/(mg·g^-1)	1.78±0.14b	1.95±0.05ab	1.94±0.11ab	2.09±0.05a	1.96±0.05ab
丙二醛含量/(μmol·L^-1)	7.48±0.52a	6.12±0.82ab	7.40±0.71a	4.94±0.23b	5.25±0.58b
CAT活性/(U·mg^-1)	1.13±0.25b	2.04±0.27a	2.09±0.34a	1.54±0.13ab	1.96±0.21a
POD活性/(U·mg^-1)	68.15±8.59b	56.05±1.81b	95.56±1.15a	96.41±3.97a	67.41±8.04b
脯氨酸含量/(μg·g^-1)	24.67±1.50a	16.26±0.12c	20.18±0.66b	25.23±0.02a	24.92±0.72a

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