基于SSR分子标记的5个黄花补血草居群花型变异及其遗传效应

doi:10.13866/j.azr.2025.03.10

Abstract

Abstract:

In heterostylous plants, the floral morph composition and frequency of populations are a consequence of mating events over generations. “Homostyly” with anthers and stigmas at the same level within a flower is a floral morph that frequently appears in the evolution of heterostyly and is often accompanied by breakdown of the heteromorphic incompatibility system and the decline of genetic diversity. To explore the formation of the H-morph and its effects on the population, we studied five Limonium aureum populations exhibiting a widely distributed floral morph (H-morph), similar to “homostyly,” in the southern margin of Tarim Basin. The floral morph composition and heterostylous syndrome were investigated by field observations and hand pollination experiments. Meanwhile, the genetic diversity and genetic structure were studied using SSR molecular markers. The results showed the following: (1) The Atushi (ATS) population consisted only of the H-morph, while the remaining four populations were composed of the long- and short-styled morph, and H-morph. All populations had a moderate level of genetic diversity, dimorphism of stigma-pollen morphology, and a strict heteromorphic incompatibility system, revealing that the floral morphs with different pollen ornamentation and stigma papilla cell morphology were compatible. (2) Genetic structure analysis, principal coordinate analysis, and phylogenetic analysis showed that the ATS population had emerged earlier and was independent of the other four populations phylogenetically, with a significant correlation between genetic distance and geographic distance. (3) The H-morphs of the two types of populations may be at different stages of the evolution of heterostyly. The self-incompatibility of the H-morph and the heteromorphic incompatibility system of the population maintained the population’s genetic diversity.

Key words: heterostyly, Limonium aureum, floral variation, genetic diversity, homostyly

ZHANG Jing, HE Shuang, ZHANG Aiqin. Floral morph variation and genetic effect of five Limonium aureum populations based on Simple Sequence Repeat molecular markers[J].Arid Zone Research, 2025, 42(3): 499-510.

Figures/Tables 11

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引物名称	上游引物序列（5′→3′）	下游引物序列（5′→3′）	等位基因大小/bp	退火温度/℃
LB6	AGAATCTGAGTGGCTGTT	GGCTTAGGGTTTGTGA	285~290	52
LB12	CAACTGTGAAAGACGGAAAG	CACGGCAATGGAGGAT	212~285	58
LB26	AGAGGGTGCCTGGAAA	AGGGTTGATGGCTTGG	134~140	53
LB30	GTGATAATGCCTGGAGA	GAGGATGTTGGTTTCG	154~180	53
LB64	CAACTGTGAAAGACGGAAAG	CACGGCAATGGAGGAT	212~284	58
Ld081	AACCGACGATCCTCTCTTCTC	AGGATTCGCCGGTCTTGT	144~150	65
Ld445	TCTCTCTCAAGCACCAGCAG	CAGACCGAAGTCAATGAGCA	196~231	60
Ln39	TGAGCCAATTAGGGCCGCTACCGAG	TCAAGACCCAATGGCTCTGCAGCAACAAAA	151	65
Ln149	GAGTGGAAATTAACCGACGGA	CTCCATCCCATCATTATTGTACTCATTGTG	90	55
Ln141	AATTCGATTGCTGCCGAACTG	AATTCATGGACAAGAAGAAGAAGAAGAAGA	189	65
Ln146	CCCGTTCTTCTCTTCCTCCCTTTG	CCATGGATAGATCCCCGCAATTAGCC	196	62
Ln152	CAGCACTTTCTATACTAAAACATCGTCGCC	AATTCGCTGGTGAGCCAACCCTATT	370	65

引物名称	N_a	N_e	I	H_o	H_e	F	F_is	F_st	N_m	PIC
LB12	5.600	2.379	1.168	0.406	0.573	0.307	0.291	0.164	1.272	0.634
LB26	2.200	1.234	0.333	0.125	0.184	0.194	0.318	0.489	0.262	0.270
LB30	2.400	1.674	0.593	0.217	0.363	0.437	0.403	0.445	0.312	0.560
LB6	3.600	1.791	0.719	0.274	0.377	0.308	0.275	0.083	2.771	0.394
LB64	3.600	1.816	0.737	0.330	0.408	0.158	0.190	0.281	0.640	0.470
Ld081	2.800	2.314	0.827	0.533	0.472	-0.106	-0.129	0.375	0.417	0.713
Ld445	2.800	2.322	0.880	0.417	0.536	0.229	0.223	0.318	0.536	0.748
Ln141	3.000	2.392	0.919	0.672	0.554	-0.209	-0.213	0.162	1.296	0.590
Ln146	2.200	1.683	0.610	0.257	0.392	0.411	0.345	0.249	0.755	0.400
Ln152	1.800	1.318	0.257	0.129	0.145	0.251	0.113	0.602	0.166	0.300
Ln39	2.400	1.673	0.379	0.187	0.188	-0.020	0.005	0.344	0.477	0.255
Mean	2.945	1.872	0.675	0.322	0.381	0.178	0.166	0.319	0.809	0.485

居群	N_a	N_e	I	H_o	H_e	F
ATS	2.636	1.833	0.619	0.290	0.360	0.293
MF	2.273	1.581	0.514	0.232	0.304	0.203
NE	3.182	2.154	0.775	0.410	0.439	0.050
WLKSY	3.636	2.105	0.834	0.410	0.455	0.137
YT	3.000	1.688	0.630	0.269	0.346	0.211
Mean	2.945	1.872	0.675	0.322	0.381	0.178

变异来源	自由度	均方和	方差组分	方差分量百分率/%
居群间	4	82.016	0.371	12
居群内	215	557.6929	2.6319	88
总计	219	693.709	3.003	100

Floral morph variation and genetic effect of five Limonium aureum populations based on Simple Sequence Repeat molecular markers

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