科尔沁沙地油松固沙林土壤真菌群落结构及功能

doi:10.13866/j.azr.2025.01.11

Abstract

Abstract:

The purpose of this study was to explore the relationship between the soil fungal community structure and function and soil chemical properties and enzyme activity in Pinus tabuliformis sand-fixing forests of different ages, so as to provide a theoretical basis for the rational management and protection of P. tabuliformis sand-fixing forests. Taking the mobile sand of Horqin Sandy Land as the control (0 year), P. tabuliformis forests with sand fixation for 18, 34, 48 and 56 years were selected as the research objects, and high-throughput sequencing technology was used to analyze the differences in soil fungal community structure and functional groups. The results show: (1) The 2517 OTUs obtained from the soil of the P. tabuliformis sand-fixing forest belong to 14 phyla, 48 classes, 127 orders, 286 families, and 579 genera of fungi. The dominant phyla were Ascomycota (47.91%-67.34%), Basidiomycota (18.45%-43.70%), and Mortierellomycota (1.41%-8.36%); the dominant genera were Biappendiculispora, Scleroderma, Tomentella, Knufia, and Amphinema. (2) Venn diagram and NMDS analysis showed that afforestation has a greater impact on soil fungal community structure. The ace index and chao index of soil fungi at each stand age increased significantly (P<0.05), and were related to organic matter, total nitrogen, and total phosphorus, urease, dehydrogenase, catalase, neutral phosphatase, sucrase and neutral protease have a significant positive correlation (P<0.05). (3) The fungal community was mainly composed of symbiotic and saprophytic types. After afforestation, the relative abundance of symbiotic types increased compared with the control, while the relative abundance of saprophytic types was relatively stable. Afforestation plays an important regulatory role on the structure and function of soil fungal communities. The research results enrich the research content of soil microbial communities in sand-fixing forests and provide a basis for soil health evaluation of P. tabuliformis sand-fixing forests in Horqin Sandy Land.

Key words: Horqin Sandy Land, Pinus tabuliformis, soil fungi, community structure, ecological function, stand age

GAO Haiyan, ZHANG Shengnan, YANG Zhiguo, ZHANG Lei, HUANG Haiguang, YAN Deren. Structure and function of soil fungal community in Pinus tabuliformis sand-fixing forests in Horqin Sandy Land[J].Arid Zone Research, 2025, 42(1): 118-126.

Figures/Tables 8

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References 33

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林龄	覆盖度/%	Shannon指数	Simpson指数	Pielou指数	chao指数	ace指数
0 a	99.95a	2.918a	0.147a	0.547a	247b	244b
18 a	99.87b	2.956a	0.149a	0.521a	372a	369a
34 a	99.87b	2.958a	0.127a	0.537a	360a	365a
43 a	99.87b	3.313a	0.090a	0.590a	386a	382a
56 a	99.85b	2.936a	0.211a	0.504a	436a	426a

指标	林龄
指标	0 a	18 a	34 a	43 a	56 a
有机质/（g·kg^-1）	2.75±1.07d	6.96±2.22c	10.78±2.94b	12.95±2.84a	11.95±3.27ab
全氮/（g·kg^-1）	0.23±0.06c	0.43±0.11b	0.56±0.19ab	0.62±0.16a	0.62±0.23a
全磷/（g·kg^-1）	0.08±0.02b	0.14±0.02a	0.16±0.05a	0.15±0.04a	0.16±0.03a
全钾/（g·kg^-1）	20.30±3.86a	22.13±1.97a	21.92±1.09a	20.98±1.68a	20.54±1.02a
速效氮/（mg·kg^-1）	9.50±2.00ab	6.28±2.91b	12.61±2.87a	12.19±1.96a	11.29±2.45ab
速效磷/（mg·kg^-1）	3.32±0.21b	6.05±0.55a	3.71±0.52b	3.19±1.00b	3.10±0.36b
速效钾/（mg·kg^-1）	60.78±3.81b	65.71±11.46b	83.07±6.81a	64.49±3.25b	85.40±10.16a
脲酶/（μg·g^-1·h^-1）	5.39±0.50d	7.82±0.33c	8.15±0.89bc	8.76±0.48b	9.72±0.46a
脱氢酶/（μg·g^-1·h^-1）	0.07±0.00c	0.25±0.09b	0.27±0.09b	0.33±0.05b	0.52±0.19a
过氧化氢酶/（mg·h^-1·g^-1）	0.40±0.04b	1.02±0.08a	0.98±0.14a	0.97±0.25a	0.99±0.18a
中性磷酸酶/（mg·h^-1·g^-1）	56.76±7.28b	111.92±44.63a	108.21±24.31a	111.76±14.17a	126.57±25.14a
蔗糖酶/（μg·h^-1·g^-1）	94.42±4.17b	180.67±37.13a	136.75±26.26ab	155.75±34.64ab	187.22±57.95a
中性蛋白酶/（μg·h^-1·g^-1）	0.19±0.05c	0.13±0.03c	0.13±0.03c	0.36±0.03b	0.91±0.05a

Structure and function of soil fungal community in Pinus tabuliformis sand-fixing forests in Horqin Sandy Land

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