天山乌鲁木齐河源1号冰川表层雪微生物多样性分析

doi:10.13866/j.azr.2023.04.16

Abstract

Abstract:

To examine the characteristics of microbial communities in the surface snow samples from the Glacier No. 1 at the headwaters of Urumqi River, Tianshan Mountains (henceforth referred to as “Urumqi Glacier No. 1”) and their relationship with climate and environment, surface snow samples were collected at an altitude of 3549 m in the spring (April, TSX1), and 3770 m (TSX2) and 3800 m (TSX3) in the summer (June) in the region in 2021. The V3-V4 region of 16S rDNA of bacteria, the V4-V5 region of 16S rDNA of archaea, and the ITS2 region of fungi were amplified by polymerase chain reaction (PCR). The products were then subjected to high-throughput sequencing, after which the microbial diversity was analyzed. The results showed that the microbial diversity of the surface snow samples from the Urumqi Glacier No. 1 differed in spring and summer, with the bacterial diversity being higher in spring and lower in summer, while the fungal diversity showed the opposite pattern. Proteobacteria (58.13%-89.10%) and Bacteroidetes (4.24%-40.74%) were the dominant bacteria at the phylum level, while Flavobacterium (2.32%-33.64%) and Polaromonas (0.01%-24.72%) were the dominant bacteria at the genus level. Thaumarchaeota (38.10%-97.55%) was the dominant archaea in the three samples, followed by Nanoarchaeaeota (0%-61.90%) and Euryarchaeota (0%-2.82%). Ascomycota (7.06%-88.43%) and Monoblepharidomycota (36.21%-40.78%) were the dominant fungi at the phylum level, and Aspergillus (0.16%-81.04%) and Rhodotorula (0.02%-8.05%) were the dominant fungi at the genus level. Network interaction analysis showed that the microbial network interaction was dominated by the positive correlation connection (97.3%), and the negative correlation connection accounted for 2.7%, and the interactive relationship tended to be cooperative. In summary, the surface snow microbiota of the Urumqi Glacier No.1 was highly diverse, and the seasonal variation in the microbial community reflects the response of microorganisms to atmospheric circulation in different seasons.

Key words: Urumqi Glacier No.1, Tianshan, surface snow, microbial diversity, network interaction analysis

ZHANG Lijuan, DU Han, YUN Fengze, MA Yinghui, ZHANG Xinqiang, Awaguli TUERSUN, MA Zhenghai. Analysis of the microbial diversity of the surface snow from Glacier No. 1 at the headwaters of Urumqi River, Tianshan Mountains[J].Arid Zone Research, 2023, 40(4): 670-680.

Figures/Tables 7

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References 52

[1]	Liu Y, Yao T, Kang S, et al. Seasonal variation of snow microbial community structure in the East Rongbuk glacier, Mt. Everest[J]. Chinese Science Bulletin, 2006, 51(12): 1476-1486.
[2]	马晓军, 刘炜, 侯书贵, 等. 不同类型冰川雪中可培养细菌多样性变化及其与环境因子关系研究[J]. 冰川冻土, 2009, 31(3): 483-489.
	[Ma Xiaojun, Liu Wei, Hou Shugui, et al. Culturable bacteria in snow pits of different type glaciers: Diversity and relationship with environment[J]. Journal of Glaciology and Geocryology, 2009, 31(3): 483-489.]
[3]	Lutz S, Anesio A M, Raiswell R, et al. The biogeography of red snow microbiomes and their role in melting arctic glaciers[J]. Nature Communications, 2016, 7(1): 11968. doi: 10.1038/ncomms11968
[4]	刘勇勤, 姚檀栋, 康世昌, 等. 珠穆朗玛峰地区东绒布冰川冰雪微生物群落及其季节变化[J]. 科学通报, 2006, 51(11): 1287-1296. doi: 10.1007/s11434-006-1287-x
	[Liu Yongqin, Yao Tandong, Kang Shichang, et al. Characteristics of bacterial community in main habitats above 6000 m on the north slope of Mount Everest[J]. Chinese Science Bulletin, 2006, 51(11): 1287-1296.] doi: 10.1007/s11434-006-1287-x
[5]	刘晓波, 康世昌, 姚檀栋, 等. 各拉丹冬峰果曲冰川雪中细菌的季节变化特征[J]. 冰川冻土, 2009, 31(4): 634-641.
	[Liu Xiaobo, Kang Shichang, Yao Tandong, et al. The seasonal change of bacterial abundance and diversity in snow of the Guoqu Glacier, Mt. Geladaindong[J]. Journal of Glaciology and Geocryology, 2009, 31(4): 634-641.]
[6]	王海伟. 天山1号冰川表面雪中细菌群落的季节性变化研究[D]. 兰州: 兰州大学, 2007.
	[Wang Haiwei. Seasonal Changes of Bacterial Flora in Surface Snow from Urumqi Glacier No.1 in the Tianshan Mountains, China[D]. Lanzhou: Lanzhou University, 2007.]
[7]	Smirnova M, Miamin U, Kohler A, et al. Isolation and characterization of fast-growing green snow bacteria from coastal East Antarctica[J]. Microbiologyopen, 2021, 10(1): e1152.
[8]	苗运玲, 于永波, 霍达, 等. 中天山北坡冬季降雪变化及其影响因子分析[J]. 干旱区研究, 2023, 40(1): 9-18.
	[Miao Yunling, Yu Yongbo, Huo Da, et al. Analysis of winter snowfall variability and its influencing factors on the north slopes of the middle Tianshan Mountains[J]. Arid Zone Research, 2023, 40(1): 9-18.]
[9]	Luo B, Sun H, Zhang Y, et al. Habitat-specificity and diversity of culturable cold-adapted yeasts of a cold-based glacier in the Tianshan Mountains, northwestern China[J]. Applied Microbiology and Biotechnology, 2019, 103(5): 2311-2327. doi: 10.1007/s00253-018-9512-5 pmid: 30483846
[10]	刘雨薇, 田伊林, 张振兴, 等. 冰川及雪线后退对河流水生生物影响的研究进展[J]. 生态科学, 2019, 38(6): 199-207.
	[Liu Yuwei, Tian Yilin, Zhang Zhenxing, et al. Research progress on the effect of retreating glaciers and snow lines on river hydrobiology[J]. Ecological Science, 2019, 38(6): 199-207.]
[11]	Xuemei L, Pei G, Qian L, et al. Muti-paths impact from climate change on snow cover in Tianshan Mountainous area of China[J]. Advances in Climate Change Research, 2016, 12(4): 303-312.
[12]	张坤, 李忠勤, 王飞腾, 等. 天山乌鲁木齐河源1号冰川积累区气溶胶和表层雪中可溶性矿物粉尘的变化特征及相互关系——以Ca²⁺、Mg²⁺为例[J]. 冰川冻土, 2008, 30(1): 113-118.
	[Zhang Kun, Li Zhongqin, Wang Feiteng, et al. Soluble mineral dusts in aerosol and surface snow on the Glacier No.1 at the headwaters of Urumqi River, East Tianshan Mountains: Characteristics and their interrelation-taking calcium and magnesium as examples[J]. Journal of Glaciology and Geocryology, 2008, 30(1): 113-118.]
[13]	陶玲, 顾燕玲, 郑晓吉, 等. 天山乌鲁木齐河源1号冰川融水可培养细菌生理生化特性及其系统发育[J]. 冰川冻土, 2015, 37(2): 511-521.
	[Tao Lin, Gu Yanlin, Zheng Xiaoji, et al. Cultivable bacteria isolated from the meltwater of the Glacier No.1 at headwater of the Urumqi River in Tianshan Mountains: Physiological-biochemical characteristics and phylogeny[J]. Journal of Glaciology and Geocryology, 2015, 37(2): 511-521.]
[14]	张寅生, 康尔泗, 刘潮海, 等. 天山乌鲁木齐河流域山区气候特征分析[J]. 冰川冻土, 1994, 16(4): 333-341.
	[Zhang Yinsheng, Kang Ersi, Liu Chaohai, et al. The climatic features of Tianshan Urumqi River Valley[J]. Journal of Glaciology and Geocryology, 1994, 16(4): 333-341.]
[15]	李宏亮, 王璞玉, 李忠勤, 等. 天山乌鲁木齐河源1号冰川东支能量-物质平衡模拟研究[J]. 冰川冻土, 2021, 43(1): 24-35. doi: 10.7522/j.issn.1000-0240.2019.1002
	[Li Hongliang, Wang Puyu, Li Zhongqin, et al. Study on the energy-mass balance simulation of the east branch of the Urumgi Glacier No.1, Tianshan Mountains[J]. Journal of Glaciology and Geocryology, 2021, 43(1): 24-35.] doi: 10.7522/j.issn.1000-0240.2019.1002
[16]	曹丽君, 孙慧兰, 兰小丽, 等. 新疆天山极端干湿事件时空演变特征[J]. 干旱区研究, 2021, 38(1): 188-197.
	[Cao Lijun, Sun Huilan, Lan Xiaoli, et al. Spatio-temporal evolution of the extreme dry and wet events in Tianshan Mountains, Xinjiang, China[J]. Arid Zone Research, 2021, 38(1): 188-197.]
[17]	Li Z, Edwards R, Mosley-Thompson E, et al. Seasonal variability of ionic concentrations in surface snow and elution processes in snow-firn packs at the PGPI site on Ürümqi glacier No. 1, eastern Tien Shan, China[J]. Annals of Glaciology, 2006, 43(1): 250-256. doi: 10.3189/172756406781812069
[18]	Zeng Q, An S. Identifying the biogeographic patterns of rare and abundant bacterial communities using different primer sets on the Loess Plateau[J]. Microorganisms, 2021, 9(1): 139. doi: 10.3390/microorganisms9010139
[19]	Wei S, Cui H, Zhang Y, et al. Comparative evaluation of three archaeal primer pairs for exploring archaeal communities in deep-sea sediments and permafrost soils[J]. Extremophiles, 2019, 23(6): 747-757. doi: 10.1007/s00792-019-01128-1 pmid: 31489482
[20]	Op De Beeck M, Lievens B, Busschaert P, et al. Comparison and validation of some ITS primer pairs useful for fungal metabarcoding studies[J]. PLoS One, 2014, 9(6): e97629. doi: 10.1371/journal.pone.0097629
[21]	Liu Y, Yao T, Kang S, et al. Seasonal variation of snow microbial community structure in the East Rongbuk glacier, Mt. Everest[J]. Chinese Science Bulletin, 2006, 51(12): 1476-1486.
[22]	Ruisi S, Barreca D, Selbmann L, et al. Fungi in Antarctica[J]. Reviews in Environmental Science & Bio/technology, 2007, 6(1-3): 127-141.
[23]	向燕, 李建光, 关梅, 等. 好氧氨氧化微生物生态学研究进展[J]. 贵州农业科学, 2012, 40(9): 115-120.
	[Xiang Yan, Li Jianguang, Guan Mei, et al. Advances in microbial ecology of aerobic ammonia-oxidizing microorganisms[J]. Guizhou Agricultural Sciences, 2012, 40(9): 115-120.]
[24]	Urakawa H, Tajima Y, Numata Y, et al. Low temperature decreases the phylogenetic diversity of ammonia-oxidizing archaea and bacteria in aquarium biofiltration systems[J]. Applied and Environmental Microbiology, 2008, 74(3): 894-900. doi: 10.1128/AEM.01529-07 pmid: 18065610
[25]	刘勇勤, 姚檀栋, 康世昌, 等. 珠穆朗玛峰北坡6000 m以上主要生境细菌群落特征[J]. 科学通报, 2007, 52(13): 1542-1547.
	[Liu Yongqin, Yao Tandong, Kang Shichang, et al. Characteristics of bacterial community in main habitats above 6000 m on the north slope of Mount Everest[J]. Chinese Science Bulletin, 2007, 52(13): 1542-1547.]
[26]	Hell K, Edwards A, Zarsky J, et al. The dynamic bacterial communities of a melting high Arctic glacier snowpack[J]. The ISME Journal, 2013, 7(9): 1814-1826. doi: 10.1038/ismej.2013.51
[27]	Xiang S R, Shang T C, Chen Y, et al. Dominant bacteria and biomass in the Kuytun 51 Glacier[J]. Applied and Environmental Microbiology, 2009, 75(22): 7287-7290. doi: 10.1128/AEM.00915-09
[28]	Zhang W, Zhang G, Liu G, et al. Diversity of bacterial communities in the snowcover at Tianshan Number 1 Glacier and its relation to climate and environment[J]. Geomicrobiology Journal, 2012, 29(5): 459-469. doi: 10.1080/01490451.2011.581329
[29]	Pester M, Schleper C, Wagner M. The Thaumarchaeota: An emerging view of their phylogeny and ecophysiology[J]. Current Opinion in Microbiology, 2011, 14(3): 300-306. doi: 10.1016/j.mib.2011.04.007 pmid: 21546306
[30]	Pitcher A, Rychlik N, Hopmans E C, et al. Crenarchaeol dominates the membrane lipids of Candidatus Nitrososphaera gargensis, a thermophilic Group I.1b Archaeon[J]. The ISME Journal, 2010, 4(4): 542-552. doi: 10.1038/ismej.2009.138
[31]	Tung H C, Bramall N E, Price P B. Microbial origin of excess methane in glacial ice and implications for life on Mars[J]. Proceedings of the National Academy of Sciences of the United States of America, 2005, 102(51): 18292-18296. pmid: 16339015
[32]	Skidmore M L, Foght J M, Sharp M J, et al. Microbial life beneath a high arctic glacier[J]. Applied and Environmental Microbiology, 2000, 66(8): 3214-3220. doi: 10.1128/AEM.66.8.3214-3220.2000 pmid: 10919772
[33]	Ma L J, Rogers S O, Catranis C M, et al. Detection and characterization of ancient fungi entrapped in glacial ice[J]. Mycologia, 2000, 92(2): 286-295. doi: 10.1080/00275514.2000.12061156
[34]	Dresch P, Falbesoner J, Ennemoser C, et al. Emerging from the ice-fungal communities are diverse and dynamic in earliest soil developmental stages of a receding glacier[J]. Environmental Microbiology, 2019, 21(5): 1864-1880. doi: 10.1111/1462-2920.14598 pmid: 30888722
[35]	Fiołka M J, Takeuchi N, Sofińska-Chmiel W, et al. Morphological and spectroscopic analysis of snow and glacier algae and their parasitic fungi on different glaciers of Svalbard[J]. Scientific Reports, 2021, 11(1): 1-18. doi: 10.1038/s41598-020-79139-8
[36]	Oliver M, Ursula P. Ectomycorrhiza of Kobresia myosuroides at a primary successional glacier forefront[J]. Mycorrhiza, 2008, 18(6-7): 355-362. doi: 10.1007/s00572-008-0188-z pmid: 18679725
[37]	Jolanta M, Frank K, Valérie H, et al. New insights into classification and evolution of the Lecanoromycetes (Pezizomycotina, Ascomycota) from phylogenetic analyses of three ribosomal RNA-and two protein-coding genes[J]. Mycologia, 2006, 98(6): 1088-1103. doi: 10.1080/15572536.2006.11832636
[38]	赵庆庆, 解金昆, 高永超, 等. 不同水文条件下黄河口滨海湿地土壤真菌群落的分布特征[J]. 环境科学学报, 2022, 42(1): 95-103.
	[Zhao Qingqing, Xie Jinkun, Gao Yongchao, et al. The distribution pattern of soil fungal community in coastal wetlands with different hydrologic conditions in the Yellow River Estuary[J]. Acta Scientiae Circumstantiae, 2022, 42(1): 95-103.]
[39]	Rosa L H, Pinto O, Coelho L C, et al. Ecological succession of fungal and bacterial communities in Antarctic mosses affected by a fairy ring disease[J]. Extremophiles, 25(5-6): 471-481. doi: 10.1007/s00792-021-01240-1
[40]	Bignell E. Aspergillus: Molecular Biology and Genomics. By Masayuki Machida and Katsuya Gomi[M]. Wiley Online Library, 2010: 336-337.
[41]	Gramss G, Voigt K D, Kirsche B. Degradation of polycyclic aromatic hydrocarbons with three to seven aromatic rings by higher fungi in sterile and unsterile soils[J]. Biodegradation, 1999, 10(1): 51-62. pmid: 10423841
[42]	王叙贤, 顾燕玲, 倪雪姣, 等. 天山乌源1号冰川表面冰尘及底部沉积层真菌群落结构比较及其系统发育分析[J]. 冰川冻土, 2017, 39(4): 781-791.
	[Wang Xuxian, Gu Yanlin, Ni Xuejiao, et al. Composition and phylogeny of fungal community in supraglacial cryoconite and subglacial sediments of the Glacier No.1 at headwaters of the Urumqi River in Tianshan Mountains[J]. Journal of Glaciology and Geocryology, 2017, 39(4): 781-791.]
[43]	Rathore M, Sinha R K, Venkatachalam S, et al. Microbial diversity and associated metabolic potential in the supraglacial habitat of a fast-retreating glacier: a case study of Patsio glacier, North-western Himalaya[J]. Environmental Microbiology Reports, 2022, 14(3): 443-452. doi: 10.1111/emi4.v14.3
[44]	Zajc J, Gostinčar C, Černoša A, et al. Stress-Tolerant Yeasts: Opportunistic pathogenicity versus biocontrol potential[J]. Genes (Basel), 2019, 10(1): 42. doi: 10.3390/genes10010042
[45]	Margesin R, Gander S, Zacke G, et al. Hydrocarbon degradation and enzyme activities of cold-adapted bacteria and yeasts[J]. Extremophiles, 2003, 7(6): 451-458. pmid: 12942349
[46]	Simon C, Wiezer A, Strittmatter Axel W, et al. Phylogenetic diversity and metabolic potential revealed in a glacier ice metagenome[J]. Applied and Environmental Microbiology, 2009, 75(23): 7519-7526. doi: 10.1128/AEM.00946-09 pmid: 19801459
[47]	刘庆, 杨蕾蕾, 周宇光, 等. 冰川细菌冷杆菌属的多样性研究进展[J]. 微生物学报, 2021, 61(4): 807-815.
	[Liu Qing, Yang Leilei, Zhou Yuguang, et al. Research progress on the diversity of glacial bacteria Cryobacterium[J]. Acta Microbiologica Sinica, 2021, 61(4): 807-815.]
[48]	Lutz S, Anesio A M, Edwards A, et al. Linking microbial diversity and functionality of arctic glacial surface habitats[J]. Environmental Microbiology, 2017, 19(2): 551-565. doi: 10.1111/1462-2920.13494 pmid: 27511455
[49]	姜远丽, 郑晓吉, 史学伟, 等. 天山冻土中嗜冷酵母菌生物多样性[J]. 食品与生物技术学报, 2012, 31(12): 1289-1294.
	[Jiang Yuanli, Zheng Xiaoji, Shi Xuewei, et al. Diversity of psychrotrophic yeast from permafrost soil at the terminus of a glacier in the Tianshan Mountains[J]. Journal of Food Science and Biotechnology, 2012, 31(12): 1289-1294.]
[50]	Christner B C, Kvitko B H, Reeve J N. Molecular identification of bacteria and eukarya inhabiting an Antarctic cryoconite hole[J]. Extremophiles, 2003, 7(3): 177-183. pmid: 12768448
[51]	Barahona S, Yuivar Y, Socias G, et al. Identification and characterization of yeasts isolated from sedimentary rocks of Union Glacier at the Antarctica[J]. Extremophiles, 2016, 20(4): 479-491. doi: 10.1007/s00792-016-0838-6 pmid: 27215207
[52]	Hassan N, Rafiq M, Hayat M, et al. Psychrophilic and psychrotrophic fungi: A comprehensive review[J]. Reviews in Environmental Science and Bio/Technology, 2016, 15(2): 147-172. doi: 10.1007/s11157-016-9395-9

样品名称	细菌			古菌			真菌
样品名称	Chao1	Shannon	Simpson	Chao1	Shannon	Simpson	Chao1	Shannon	Simpson
TSX1	297.1	3.48	0.05	7.5	1.31	0.36	148.1	1.14	0.38
TSX2	228.1	2.95	0.09	10.5	1.47	0.34	526.1	2.75	0.18
TSX3	279.0	3.06	0.08	11	1.44	0.39	443.1	2.33	0.23

Analysis of the microbial diversity of the surface snow from Glacier No. 1 at the headwaters of Urumqi River, Tianshan Mountains

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