青海云杉(Picea crassifolia Kom.),种子生活力,温度,光照,水分胁迫,盐分胁迫,萌发率,天祝," /> 青海云杉(Picea crassifolia Kom.),种子生活力,温度,光照,水分胁迫,盐分胁迫,萌发率,天祝,"/> Picea crassifolia Kom.,seed vigor,temperature,light,moisture stress,salinity stress,germination rate,Tianzhu,"/> 青海云杉种子萌发特性
干旱区研究

干旱区研究 ›› 2013, Vol. 30 ›› Issue (5): 877-881.

• 植物生理 • 上一篇    下一篇

青海云杉种子萌发特性

刘有军, 刘世增, 康才周, 满多清, 李德禄, 李银科   

  1. 甘肃省荒漠化与风沙灾害防治省部共建国家重点实验室 甘肃省治沙研究所，甘肃兰州 730000
  • 收稿日期:2012-07-02 修回日期:2012-09-10 出版日期:2013-09-15 发布日期:2013-09-22
  • 通讯作者: 刘世增.E-mail： shzliu@126.com
  • 作者简介:刘有军(1977-)，男，助理研究员，主要研究方向为荒漠植物种子生态学.E-mail： lyjsmallgrass@163.com
  • 基金资助:

    国家自然科学基金项目（30960062）；甘肃省技术研究与开发专项计划项目(0709TCYH035);国家林业局公益性科研院所专项资金项目（CAFYBB200700X）

Seed Germination of Picea crassifolia Kom.

 LIU  You-Jun, LIU  Shi-Zeng, KANG  Cai-Zhou, MAN  Duo-Qing, LI  De-Lu, LI  Yin-Ke   

  1. State Key Laboratory of Desertification and Aeolian Sand Disaster Combating, Gansu Province Institute 
    of Desert Control Research, Lanzhou 730000, China
  • Received:2012-07-02 Revised:2012-09-10 Online:2013-09-15 Published:2013-09-22

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被引次数

11

摘要： 以天祝县青海云杉天然种群种子为研究对象，通过对种子生活力测定，分析种子萌发对温度、光照、水分和盐分胁迫的响应，为引种提供参考数据。结果表明：① 种子的生活力为72%。② 在黑暗条件下，温度小于10 ℃种子不萌发，最适萌发温度25/15 ℃(72%)；种子在24 h黑暗和24 h光照条件下的萌发率显著高于14 h光照/10 h黑暗。③ 当NaCl胁迫浓度为50 mmol·L-1时，可促进种子萌发，当胁迫浓度大于50 mmol·L-1时，抑制种子萌发；胁迫解除后，随着胁迫浓度的增加，恢复萌发率(RGR)先增加后减小，胁迫浓度为400 mmol·L-1时，RGR达到最大值13%。④ 随着PEG-6000试剂胁迫渗透势的增加，种子萌发显著减少，在-0.9 MPa时，种子不能萌发；胁迫解除后，RGR先增加后减少，在-0.9 MPa时，达到最大值35%。

关键词: 青海云杉(Picea crassifolia Kom.)')">青海云杉(Picea crassifolia Kom.), 种子生活力, 温度, 光照, 水分胁迫, 盐分胁迫, 萌发率, 天祝

Abstract: Seeds were collected from the natural population of Picea crassifolia Kom. in Tianzhu County, Wuwei City, Gansu Province, China in November 2010. Seed vigor was tested by TTC, the experiments were conducted to investigate the response of seed germination to temperature, light, moisture stress and salinity stress, and some references were finally provided for transplanting P. crassifolia Kom. from Tianzhu to Minqin County. The results are as follows: ① The vigor of P. crassifolia Kom. seeds was 72%; ② Under a complete dark, seeds could not germinate when temperature was lower than 10 ℃, and the optimal temperature for germination was 25/15 ℃ (72%); germination rate of P. crassifolia Kom. seeds under the 24-hour complete dark and 24-hour light were significantly higher than that under 14-hour light and 10-hour dark; ③ Seed germination could be improved when NaCl concentration was 50 mmol·L-1, but it could be restricted when NaCl concentration was higher than 50 mmol·L-1; seed germination could be recovered after salinity stress was removed, the recovery germination rate (RGR) was increased at first and then decreased with the increase of NaCl concentration, and RGR reached its maximum (13%) when NaCl concentration was 400 mmol·L-1; ④ Seed germination was significantly decreased with the increase of PEG-6000 osmotic potential, and seeds could not germinate when the osmotic potential was -0.9 MPa; after PEG-6000 stress was removed, RGR was increased at first and then decreased with the increase of PEG-6000 concentration, and it reached its maximum (35%) when the osmotic potential was -0.9 MPa.

Key words: Picea crassifolia Kom.')">Picea crassifolia Kom., seed vigor, temperature, light, moisture stress, salinity stress, germination rate, Tianzhu