骆驼刺（Alhagi sparsifolia Shap.）,高效液相,指纹图谱," /> 骆驼刺（Alhagi sparsifolia Shap.）,高效液相,指纹图谱,"/> Alhagi sparsifolia Shap., HPLC,fingerprint chromatogram,"/> 骆驼刺高效液相指纹图谱研究
干旱区研究

干旱区研究 ›› 2013, Vol. 30 ›› Issue (5): 873-876.

• 植物生理 • 上一篇    下一篇

骆驼刺高效液相指纹图谱研究

赛那瓦尔•芒思尔, 阿里木江•阿布力孜 , 邹国安   

  1. 中国科学院新疆理化技术研究所，中国科学院干旱区植物资源化学重点实验室，新疆 乌鲁木齐 830011；
    省部共建新疆特有药用资源利用国家重点实验室培育基地，新疆 乌鲁木齐 830011
  • 收稿日期:2013-06-26 修回日期:2013-08-05 出版日期:2013-09-15 发布日期:2013-09-22
  • 作者简介:赛那瓦尔?芒思尔(1985-)，女，维吾尔族，在读博士，主要从事有机化学研究.E-mail: sanam0405@163.com
  • 基金资助:

    中国科学院“西部之光”人才培养计划“西部博士资助项目”（XBBS200917）；新疆维吾尔自治区“草花、骆驼刺、毛菊等8种新疆特色维药药材化学对照的制备”(20100108)

HPLC Fingerprint Chromatogram of Alhagi sparsifolia Shap.

 MANSUR Sanawar,ABULIZE Alimujiang, ZOU  Guo-An   

  1. The Key Laboratory of Plant Resources and Chemistry of Arid Zone; State Key Laboratory Base of Xinjiang Indigenous Medicinal Plant
    Resources Utilization,Xinjiang Technical Institute of Physics and Chemistry,Chinese Academy of Sciences,Urumqi 830011,China
  • Received:2013-06-26 Revised:2013-08-05 Published:2013-09-15 Online:2013-09-22

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摘要： 建立骆驼刺药材反相高效液相色谱指纹图谱分析方法，为有效控制骆驼刺药材的质量奠定基础。采用反相高效液相色谱，色谱柱为Waters Atlantis T3 C18（4.6 mm×250 mm，5 μm），用乙腈-0.2%甲酸水溶液线性梯度洗脱，以 1.0 mL·min-1为流速，检测波长为 280 nm，采集时间为 100 min，分析了10批骆驼刺的HPLC指纹图谱。得到了分离度较好的骆驼刺药材高效液相色谱指纹图谱，标定出了11个共有峰，方法学考察结果符合指纹图谱技术要求。确定的指纹图谱研究方法稳定、可靠，可用于骆驼刺药材的质量分析。

关键词: 骆驼刺（Alhagi sparsifolia Shap.）')">骆驼刺（Alhagi sparsifolia Shap.）, 高效液相, 指纹图谱

Abstract: Alhagi sparsifolia Shap.is a perennial subshrub in the Leguminosae family.It grows mainly in Central Asia and Xinjiang Uygur Autonomous Region,China,and has been used in traditional Uygur medicine for a long time.It is widely used for treating physically weak,dizzy,cold,headache,intestinal constipation and arthrodynia.It mainly contains flavonoids,sterols, alkaloids, fatty acids and polysaccharides.There is no any report on the fingerprint chromatogram or chemical marker of A.sparsifolia yet.In order to develop the fingerprint chromatogram and provide the basis for the effective control of A.sparsifilia quality, the samples were separated by RPHPLC on a Waters Atlantis T3 C18 (4.6 mm×250 mm, 5 μm), with acetonitrile and 0.2% formic acid as mobile phase in a linear gradient manner.The flow rate was 1.0 mL·min-1, the wavelength of detection was 280 nm, and the acquisition time was 100 min.HPLC fingerprint chromatograms of 10 batches of A.sparsifolia were analyzed.The HPLC fingerprint chromatogram of A.sparsifolia was developed with satisfactory resolution and 11 common fingerprint peaks with high similarity of correlation coefficients above 0.9.The peak No.10 and peak No.11 were the main compounds of A. sparsifolia, with the retention time matching isorhamnetin→3→O→β→D→galactosyl→(6→1) →α→L→rhamnoside and isorhamnetin→3→O→β→Drutinoside by HPLC, respectively. The results met the requirements of fingerprint technology. It was identified that the fingerprint method was stable and reliable, and could be used for the quality control of medicinal A. sparsifolia.

Key words: Alhagi sparsifolia Shap.')">Alhagi sparsifolia Shap., HPLC, fingerprint chromatogram