石油烃降解混合菌修复稠油污染土壤的影响因素

干旱区研究 ›› 2013, Vol. 30 ›› Issue (4): 603-608.

石油烃降解混合菌修复稠油污染土壤的影响因素

董亚明^1,2, 刘其友¹, 赵东风¹, 赵朝成¹, 高泊³

1.中国石油大学化学工程学院,山东青岛 266555;
2.新疆维吾尔自治区环境保护厅,新疆乌鲁木齐 830063;
3.新疆环境监测总站,新疆乌鲁木齐 830011

收稿日期:2012-07-26 修回日期:2012-10-15 发布日期:2013-07-29
作者简介:董亚明(1973－),男,博士研究生,主要从事石油污染土壤生物修复研究. E-mail: doym@163.com
基金资助:
中央高校基本科研业务费专项资金资助(27R1204018A)

Affecting Factors of Bioremediation in Improving Soil Contaminated by Thickened Oil

DONG Ya-ming^1,2, LIU Qi-you¹, ZHAO Dong-feng¹, ZHAO Chao-cheng¹, GAO Bo³

1. College of Chemical Engineering, China University of Petroleum Huadong, Qingdao 266555, Shandong, China;
2. Xinjiang Bureau of Environmental Protection, Urumqi 830063, Xinjiang, China;
3. Xinjiang Environmental Monitor Center, Urumqi 830011, Xinjiang, China

Received:2012-07-26 Revised:2012-10-15 Online:2013-07-29

摘要/Abstract

摘要： 稠油在开采、贮运、炼制加工及使用过程中,不可避免的污染土壤环境,对人体健康和环境造成严重影响,因此有必要采取有效措施对稠油污染土壤进行修复。利用筛选获得的石油烃降解混合菌KL9-1,对稠油污染土壤进行修复研究,考察修复过程中的影响因素。结果表明:pH、接种量、土壤中N/P、表面活性剂用量、翻耕频率、浇水频率和膨松剂种类等因素对污染土壤修复有明显的影响。在每千克稠油污染土壤体系中,控制初始pH为8.0,接种量为70.0 mL,N∶P为3∶1,表面活性剂用量为4.0 g,每2 d浇水1次,4 d翻耕1次,稻壳作为膨松剂,在此条件下经过70 d的生物修复,石油烃降解率最高可达54.07%。

关键词: 土壤, 稠油污染, 石油烃降解菌, 生物修复

Abstract: Thickened oil causes inevitably the serious contamination of edatope and impacts human health and environment in its mining, storage, transportation, refining and using process. It is necessary to take effective measures for improving soil contaminated by thickened oil. The microbial screened consortium KL9-1 was used to research the bioremediation for improving soil contaminated by thickened oil, and the factors in the biodegradation progress were studied. The results showed that the pH value, inoculum size, soil N/P, surfactant dosage, tillage frequency, watering frequency and raising agent affected significantly the bioremediation. In the soil contaminated by thickened oil per kilogram, the initial conditions of pH of 8.0, inoculum of 70.0 mL, N/P of 3∶1 and surfactant dosage of 4.0 g were controlled, the soil was watered every two days and plowed every four days, rice husks were used as a raising agent, and the petroleum hydrocarbon degradation rate could be as high as 54.07% after carrying out a biodegradation for 70 days.

Key words: soil, contaminated by thickened oil, hydrocarbon degrading bacterium, bioremediation

董亚明, 刘其友, 赵东风, 赵朝成, 高泊. 石油烃降解混合菌修复稠油污染土壤的影响因素[J]. 干旱区研究, 2013, 30(4): 603-608.

DONG Ya-ming, LIU Qi-you, ZHAO Dong-feng, ZHAO Chao-cheng, GAO Bo. Affecting Factors of Bioremediation in Improving Soil Contaminated by Thickened Oil[J]. Arid Zone Research, 2013, 30(4): 603-608.

参考文献

[1] Gobran B D,Oil A,Co G,et al.Absolute permeability as a function of confining pressure,pore pressure,and temperature[J]. SPE Formation Evaluation,1987,2(1):77-84.
[2] 刘有军,马全林,张德魁,等.腾格里沙漠南缘3种一年生植物出苗率对沙埋和GA₃的响应[J]. 干旱区研究,2010,27(5):699-704.
[Liu Youjun,Ma Quanlin,Zhang Dekui,et al.Response of germination rate of three annual plant species to sand burial depths and GA₃ in southern marginal zone of the Tengger desert[J]. Arid Zone Research,2010,27(5):699-704.]
[3] 贾学军.高粘度稠油开采方法的现状与研究进展[J]. 石油天然气学报,2008,30(2):529-531.
[Jia Xuejun.The present methods and research progress in high viscosity of heavy oil production situation[J]. Journal of Oil and Gas Technology,2008,30(2):529-531.]
[4] 于连东.世界稠油资源的分布及其开采技术的现状与展望[J]. 特种油气藏,2001,8(2):98-103.
[Yu Liandong.Distribution of world heavy oil reserves and its recovery technologies and futrue[J]. Special Oil and Gas Reservoirs,2001,8(2):98-103.]
[5] Whang L M,Liu P W,Ma C C,et al.Application of biosurfactants,rhamnolipid,and surfactin,for enhanced biodegradation of diesel-contaminated water and soil[J]. Journal of Hazardous Materials,2008,151(1):155-163.
[6] 魏小芳,张忠智,罗一菁,等.重质石油污染土壤的生物修复[J]. 化学与生物工程,2005,22(7):7-9.
[Wei Xiaofang,Zhang Zhongzhi,Luo Yijing,et al.Bioremediation of heavy oil contaminated soil[J]. Chemistry and Bioengineering,2005,22(7):7-9.]
[7] 程国玲,李培军.石油污染土壤的植物与微生物修复技术[J]. 环境工程学报,2007,1(6):91-96.
[Cheng Guoling,Li Peijun,Phytoremediation and microbial remediation of petroleum contaminated soil[J]. Chinese Journal of Environmental Engineering,2007,1(6):91-96.]
[8] 刘五星,骆永明,滕应,等.石油污染土壤的生物修复研究进展[J]. 土壤,2006,38(5):634-639.
[Liu Wuxing,Luo Yongming,Teng Ying,et al.Advances in bioremediation of petroleum contaminated soil[J]. Soils,2006,38(5):634-639.]
[9] 谭丽泉,仇永亮.石油污染土壤的生物修复技术[J]. 牡丹江大学学报,2007,16(5):77-79.
[Tan Liquan,Qiu Yongliang.Technologies in bioremediation of oil pollution soil[J]. Journal of Mudanjiang University,2007,16(5):77-79.]
[10] Alexander M.Biodegradation and Bioremediatio[M]. San Diego,CA,USA:Academic Press,1999.
[11] Rittmann B E,Mccarty P L.Environmental Biotechnology:Principles and Application[M]. New York,USA:McCraw-Hill,2001.
[12] Atlas R M.Microbial degradation of petroleum hydrocarbons:An environmental perspective[J]. Microbiological Reviews,1981,45(1):180-209.
[13] 赵晴.疏水性石油烃降解菌强化降解系统的构建及其降解能力研究[D]. 武汉:武汉大学,2005.
[Zhao Qing.Study on Construction of Bioaugmentation Degradative System and Degradation Capability for Hydrophobic Hydrocarbon Degrading Bacteria[D]. Wuhan:Wuhan University,2005.]
[14] Dibble J T,Barth A R.Effect of environmental parameters on the bio-degradation of oil sludge[J]. Applied Environmental Microbiology,1979,37:729-739.
[15] 徐玉林.石油污染土壤降解与土壤的环境关系[J]. 农机化研究,2004,11(6):86-88.
[Xu Yulin.The relations between the reductions of the soil contaminated by petroleum hydrocarbon and the soil environment[J]. Journal of Agricultural Mechanization Research,2004,11(6):86-88.]