干旱胁迫对银沙槐幼苗叶绿体和线粒体超微结构及膜脂过氧化的影响

doi:10.13866/j.azr.2016.01.15

Abstract

Abstract: Ammodendron argenteum is unique rare and endangered desert plant in Xinjiang.There are research evidences showing its growing traits and physiological characteristics and so on,however research is lacking to understand the response of ultrastructure under drought stress.Using transmission electron microscopy and measurement of MDA content and membrane permeability,the change on ultrastructure of mesophyll cells and hypocotyl cells of Ammodendron argenteum seedlings were observed.And the effects of drought stress on ultrastructure and membrane lipid peroxidation were analyzed.The result showed that chloroplasts deformed,outer membranes were wavy and incomplete.In the meanwhile,membrane structures of grana and stroma-thylakoids blurred,grana bent and dilated.The mitochondrion outer membranes were disrupted and cristaes disappeared,and the lumen formed a cavity.The membrane permeability and MDA content increased and significantly higher than the control(P＜0.05),cell membrane structure were damaged,the effects of membrane lipid peroxidation intensified.Membrane permeability and MDA content of cotyledon cells increased and significantly higher than those of hypocotyl cells (P＜0.05),and the changes of ultrastructure in cotyledon cells was earlier than hypocotyl cells,which showed that drought tolerance of hypocotyl stronger than cotyledon's.Mitochondrial damaged later than chloroplasts in cotyledon which showed that drought tolerance of Mitochondrial stronger than chloroplasts'.The responsive difference under drought stress between different structures and different organelles in the same structure may be a kind of defense reaction which plant tissue cells have in the adverse environment.The change of ultrastructure under drought stress reflected the disorder and damage of intracellular membrane systems,and the damage of the membrane systems might be the result of intensified lipid peroxidation.

Key words: Ammodendron argenteum, drought stress, chloroplast, mitochondria, ultrastructure, membrane lipid peroxidation

XU Ping, LI Jin, LV Hai-ying, LI Yong-jie, LI Jia, MA Chun-lan. Effects of Drought Stress on Ultrastructure of Chloroplast and Mitochondria and Membrane Lipid Peroxidation of Ammodendron argenteum[J].Arid Zone Research, 2016, 33(1): 120-130.

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Effects of Drought Stress on Ultrastructure of Chloroplast and Mitochondria and Membrane Lipid Peroxidation of Ammodendron argenteum

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