野大麦对干旱胁迫的生理响应与转录组分析

doi:10.13866/j.azr.2019.04.15

Abstract

Abstract: The physiological response of Hordeum brevisubulatum to drought stress was researched so as to identify the drought tolerance related to the genes,physiological indexes and transcriptome sequencing of H.brevisubulatum seedlings under different concentrations of polyethylene glycol (PEG) 6000 stress.The results showed that the content of proline in leaves of H.brevisubulatum was increased continuously after the PEG 6000 stress for 2 days,and the activity of soluble sugar and superoxide dismutase (SOD) was increased at first and then decreased.The membership function method was used to select the transcriptome analysis and sequencing of H.brevisubulatum leaves under the treatment of 30 mmol·L^-1 PEG 6000.Compared with the control group,the 6 868 up-regulated genes and 2 081 down-regulated genes were identified.The differentially expressed genes were classified into three categories and 54 functional groups by Go function classification.The 6 579 differentially expressed genes were enriched into 136 pathways by KEGG pathway enrichment.Arginine and proline metabolism,glycolysis and gluconeogenesis,starch and sucrose metabolism,peroxisome pathways and other pathways,and the related up-regulated expression genes in various metabolic pathways were discovered.

Key words: Hordeum brevisubulatum, drought stress, physiological response, RNA-Seq, Zhalong Wetland, Songnen Plain

SONG Shi-wei, JIAO De-zhi, CHEN Xu, ZHAO Ze-long, YANG Yun-fei. Physiological Response and Transcriptome of Hordeum brevisubulatum to Drought Stress[J].Arid Zone Research, 2019, 36(4): 909-915.

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Physiological Response and Transcriptome of Hordeum brevisubulatum to Drought Stress

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