干旱区研究 ›› 2022, Vol. 39 ›› Issue (5): 1618-1630.doi: 10.13866/j.azr.2022.05.26
收稿日期:
2022-01-24
修回日期:
2022-04-22
出版日期:
2022-09-15
发布日期:
2022-10-25
通讯作者:
刘秉儒
作者简介:
牟红霞(1996-),女,硕士研究生,主要从事植物生态学研究. E-mail: 基金资助:
MOU Hongxia1(),LIU Bingru2(),LI Zihao2,LI Guoqi1,MA Dongmei1
Received:
2022-01-24
Revised:
2022-04-22
Online:
2022-09-15
Published:
2022-10-25
Contact:
Bingru LIU
摘要:
为探究矿井水排放对荒漠草原土壤微生物群落结构及多样性的影响,以宁夏干旱风沙区矿井水排放地的荒漠草原为研究对象,通过高通量测序分析矿井水排放湖泊沿岸水滨区域(MJTA)、近岸陆域(MJTB)和自然区域(MJTC)土壤中0~10 cm、10~20 cm和20~30 cm土层中细菌和真菌群落组成,结合土壤理化因子等生境因素,揭示矿井水干扰对土壤微生物群落结构组成及多样性影响的主要因素。结果表明:(1) 矿井水排放显著改变了土壤细菌和真菌的群落组成,对土壤细菌和真菌群落门水平相对丰度差异影响显著。(2) 水滨区域、近岸陆域和自然区域中主要优势细菌门均为放线菌门(Actinobacteria)和变形菌门(Proteobacteria),主要优势细菌属均为: norank_f__norank_o__norank_c__MB-A2-108;水滨区域、近岸陆域和自然区域中主要优势真菌门均为子囊菌门(Ascomycota),水滨区域主要优势真菌属为unclassified_c__Sordariomycetes,近岸陆域和自然区域主要优势真菌属为光黑壳属(Preussia)。(3) 矿井水外排导致荒漠草原水滨区域土壤盐分、水分显著高于近岸陆域和自然区域,盐分抑制了土壤细菌和真菌的生长,但同时促进了研究区嗜盐碱细菌的富集。(4) 水滨区域土壤细菌和真菌多样性与丰富度均显著低于自然区域,进一步说明矿井水对土壤细菌和真菌丰富度与多样性影响显著;不同土层土壤细菌和真菌的丰富度与多样性差异较小;土壤环境因子与土壤细菌和真菌多样性分析表明土壤盐分、含水量、有机碳和pH是影响荒漠草原土壤细菌和真菌群落多样性的主要影响因子。
牟红霞,刘秉儒,李子豪,李国旗,麻冬梅. 矿井水对荒漠草原土壤微生物群落结构及多样性的影响[J]. 干旱区研究, 2022, 39(5): 1618-1630.
MOU Hongxia,LIU Bingru,LI Zihao,LI Guoqi,MA Dongmei. Effects of mine water on soil microbial community structure and diversity in desert steppe[J]. Arid Zone Research, 2022, 39(5): 1618-1630.
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