氮添加对天山高寒草原土壤酶活性和酶化学计量特征的影响

Abstract

Abstract: The effects of nitrogen (N) addition on activities of soil carbon (C),N,and phosphorus (P) circulationrelated enzymes were investigated in an alpine grassland ecosystem in 2018.A longterm simulated N addition experiment was initiated at the Bayinbuluk Grassland Ecological Research Station of the Chinese Academy of Sciences in 2009;four N fertilizer treatments were applied at different sites:control (N0,0 kg·hm^-2·a^-1),low nitrogen (N1,10 kg·hm^-2·a^-1),moderate nitrogen (N3,30 kg·hm^-2·a^-1),and high nitrogen (N9,90 kg·hm^-2·a^-1).In the present study,stoichiometric ratios of soil ecological enzymes and the relationships between soil enzyme activities and environmental factors were analyzed.Our results revealed the following.Compared with N0,N3 treatment significantly increased β-1,4-glucosidase (BG),cellobiohydrolase (CBH) and β-1,4-xylosidase activities (βx) (P<0.05).N1 and N3 treatments obviously increased phenol oxidase (PPO) activity ( P < 0.05).N3 treatment obviously increased β-1,4-Nacetylglucosaminidase (NAG) activity ( P <0.05).No N treatment affected Lleucine aminopeptidase activity (LAP).Correlation analysis showed that activities of all soil enzymes were positively correlated to soil organic C (SOC) content (except NAG) and total phosphorus (TP) content but were not to total nitrogen content.Soil ecoenzymatic C∶N∶P stoichiometry in the study area was 1∶1∶1.2,which is inconsistent with the global 1∶1∶1 pattern.This result suggests that soil microbial growth in the studied grasslands is limited by P content.Canonical redundancy analysis indicated that SOC and TP were the dominant factors affecting soil enzyme activity.

Key words: nitrogen addition, soil enzyme activity, soil ecoenzymatic stoichiometry, nutrient limitation, alpine grassland, Bayinbuluk

LE Jiajia, SU Yuan, PENG Qingwen, GENG Fengzhan, HAN Wenxuan, LI Wenli, LI Kaihui, LIU Xuejun. Effects of nitrogen addition on soil enzyme activities and ecoenzymatic stoichiometry in alpine grassland of the Tianshan Mountains[J].Arid Zone Research, 2020, 37(2): 382-389.

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Effects of nitrogen addition on soil enzyme activities and ecoenzymatic stoichiometry in alpine grassland of the Tianshan Mountains

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