断根与IBA处理对沙冬青根系质量及幼苗生长的影响

doi:10.13866/j.azr.2022.01.22

Abstract

Abstract:

To solve the technical problems associated with forestry of Ammopiptanthus mongolicus, i.e., the long main root, few capillaries, difficult rooting, and low transplantation survival rate, we conducted root cuttings at different intensities and added different concentrations of IBA to A. mongolicus seedlings with different degrees of growth development. The effects of different treatment combinations on root morphology, root quality, and seedling growth were then measured and analyzed after root culture. This investigation was aimed at identifying the key measures for promoting the root survival of A. mongolicus seedlings and thereby providing a knowledge base for cultivating A. mongolicus seedlings and developing afforestation techniques. The results were as follows. (1) Root cutting promoted the occurrence of multimain roots in A. mongolicus, as well as an increase in the lateral roots, total roots, aboveground and underground biomass, root-shoot ratio, root activity, and root cation exchange capacity (CEC). Among the treatments, the 0.5-cm root tip treatment (F2) significantly promoted an increase in lateral root number, root tip number, branch number, seedling quality index, and root activity when the root length reached 1.5-2.0 cm. Root activity reached a peak value with the root cutting treatment (137.70% higher than the value in the control). Root CEC was maximal when root length reached 4.5-6.0 cm. (2) The effect of hormones on promoting root and seedling growth was further improved after root cutting. The root morphological index (except the average root length) and root CEC were significantly increased after 1.5-2.0 cm long roots were treated with 0.5 cm cutting + 0.075 mg·L ^-1 IBA/0.125 mg·L^-1 IBA (P3 and P4 treatments, respectively). Seedling fresh weight, root dry-weight, root-shoot ratio, seedling quality index, and root activity were highest under the P4 treatment. Compared with the control, the content of soluble sugar and NSC in roots under P3 treatment was significantly increased by 26.04% and 29.07%, respectively. Additionally, the content of soluble protein in roots reached its peak under the P4 treatment (13.27 mg·g^-1). Proper root cutting and the addition of IBA can promote root absorption and nutrient storage of A. mongolicus by altering root morphology and improving root activity, which in turn improves root quality and the growth potential of seedlings.

Key words: Ammopiptanthus mongolicus, cutting roots, hormone treatment, root morphology, root activity, seedling growth

WANG Jingjing,YAN Haibing,WANG Zixuan,XIE Tiantian,YANG Xiuqing. Effects of root cutting and IBA treatment on the root quality and growth of Ammopiptanthus mongolicus seedlings[J].Arid Zone Research, 2022, 39(1): 230-239.

Figures/Tables 8

Tab.1

Fig. 1

Tab. 2

Tab. 3

Analysis of growth index of Ammopiptanthus mongolicus seedlings"

处理	苗高/cm	地径/mm	地上部分鲜重/g	根鲜重/g	根冠比	根干重/g	叶干重/g	地上部分干重/g	苗木质量指数
F1	5.496±0.811ab	1.512±0.084ab	0.276±0.027a	0.035±0.012b	0.109±0.028a	0.003±0.001a	0.035±0.002ab	0.044±0.005ab	0.007±0.001a
F2	5.774±0.486ab	1.588±0.105ab	0.279±0.021a	0.105±0.014bc	0.376±0.044a	0.007±0.001b	0.038±0.003ab	0.041±0.002ab	0.012±0.002b
S1	5.982±0.262ab	1.103±0.127a	0.256±0.015a	0.040±0.014ab	0.156±0.043a	0.002±0.009a	0.044±0.002b	0.047±0.006b	0.007±0.003a
S2	4.210±0.221a	1.108±0.090a	0.277±0.016a	0.045±0.006b	0.162±0.022a	0.004±0.001a	0.038±0.002ab	0.045±0.003b	0.009±0.001a
S3	6.083±0.333b	1.563±0.050ab	0.278±0.014a	0.064±0.007b	0.230±0.026a	0.004±0.001a	0.037±0.005ab	0.042±0.008ab	0.009±0.001a
T1	5.487±0.135e	1.183±0.086a	0.237±0.016a	0.045±0.006b	0.190±0.016a	0.003±0.001a	0,038±0.002ab	0.045±0.002b	0.007±0.001a
T2	4.297±0.292a	1.500±0.075ab	0.271±0.022a	0.064±0.004ab	0.236±0.015a	0.006±0.002ab	0.026±0.002a	0.040±0.002ab	0.009±0.001a
T3	5.969±0.285cd	1.529±0.084ab	0.272±0.024a	0.059±0.005ab	0.217±0.017a	0.004±0.002a	0.026±0.002a	0.030±0.003a	0.007±0.001a
T4	6.460±0.299b	1.542±0.061ab	0.276±0.022a	0.064±0.004a	0.232±0.029a	0.005±0.002a	0.033±0.002ab	0.035±0.004ab	0.009±0.001a
P1	2.591±0.171a	1.733±0.244bc	0.283±0.027a	0.123±0.038c	0.435±0.063a	0.008±0.003b	0.017±0.002a	0.025±0.003a	0.013±0.002b
P2	2.910±0.271a	1.594±0.015ab	0.280±0.016a	0.129±0.011c	0.460±0.106a	0.008±0.002b	0.023±0.004a	0.028±0.002a	0.015±0.001bc
P3	4.254±0.363a	1.615±0.058b	0.323±0.021b	0.268±0.044d	0.830±0.145b	0.010±0.003bc	0.030±0.002a	0.031±0.001a	0.015±0.001bc
P4	4.080±0.435a	1.785±0.061bc	0.351±0.040b	0.363±0.060d	1.034±0.102b	0.018±0.001c	0.018±0.004a	0.027±0.001a	0.016±0.001c

Tab. 3

Fig. 2

Fig. 3

Fig. 4

Tab. 4

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处理	平均根长/cm	总根投影面积/cm²	根表面积/cm²	根平均直径/mm	总根体积/cm³	侧根数	根尖数	分支数
F1	15.26±7.96c	1.14±0.30ab	3.57±0.93a	0.58±0.09ab	0.04±0.01a	8.10±1.90a	12±2a	8±1a
F2	11.99±1.87bc	1.18±0.04ab	3.72±1.70a	0.46±0.05a	0.05±0.01a	14.00±2.36b	21±3b	15±2b
S1	16.12±2.28c	1.38±0.10a	4.35±1.89a	0.60±0.10ab	0.06±0.03a	10.60±2.15a	15±2a	12±2a
S2	8.14±5.66a	1.46±0.05a	4.59±1.40a	0.53±0.14a	0.07±0.03a	11.33±1.54a	15±1a	13±1ab
S3	7.45±7.19a	1.57±0.04ab	4.94±1.69ab	0.50±0.06a	0.06±0.02a	12.18±1.29ab	20±3ab	13±3ab
T1	17.60±6.61c	1.66±0.16bc	5.16±1.07bc	0.65±0.20b	0.08±0.01bc	10.91±1.28a	17±2ab	9±1a
T2	9.51±9.29ab	1.54±0.13bc	4.91±1.99b	0.64±0.13b	0.07±0.01bc	14.70±0.73b	17±1ab	14±1ab
T3	15.87±3.78c	0.84±0.14ab	2.64±0.08a	0.46±0.08a	0.03±0.01a	13.78±1.87ab	23±1b	12±1a
T4	5.27±4.99a	0.79±0.07a	2.47±0.83a	0.59±0.10b	0.04±0.01ab	15.10±2.04b	20±2ab	14±2b
P1	5.65±5.42a	1.61±0.04ab	5.06±0.64ab	0.65±0.06bc	0.09±0.05ab	15.50±1.88b	26±4b	20±3bc
P2	5.73±8.22a	2.29±0.52b	7.20±1.63bc	0.57±0.02ab	0.10±0.0ab	19.50±1.81b	41±3bc	25±5c
P3	11.95±20.79bc	4.77±0.41c	14.99±3.42d	0.66±0.05bc	0.25±0.08c	94.25±7.65d	98±9d	107±26e
P4	4.15±21.87a	3.54±0.53bc	11.13±4.81c	0.71±0.11c	0.18±0.09bc	60.75±4.19c	67±6c	91±19d

指标	RP	RS	RV	LRN	Tips	Forks	RA	CEC	R SS:S	R NSC	RSP	RSS
RP	1
RS	1.000^**	1
RV	0.992^**	0.992^**	1
LRN	0.949^**	0.949^**	0.957^**	1
Tips	0.956^**	0.956^**	0.956^**	0.981^**	1
Forks	0.951^**	0.951^**	0.957^**	0.983^**	0.972^**	1
RA	0.728^**	0.729^**	0.743^**	0.722^**	0.799^**	0.772^**	1
CEC	0.593^*	0.593^*	0.581^*	0.712^**	0.720^**	0.719^**	0.572^*	1
R SS:S	0.209	0.204	0.263	0.277	0.308	0.266	0.290	0.460	1
R NSC	0.249	0.245	0.325	0.382	0.396	0.327	0.285	0.364	0.848^**	1
RSP	0.328	0.330	0.320	0.385	0.325	0.464	0.344	0.444	-0.330	-0.350	1
RSS	0.212	0.210	0.289	0.370	0.365	0.285	0.196	0.169	0.470	0.864^**	-0.290	1

Effects of root cutting and IBA treatment on the root quality and growth of Ammopiptanthus mongolicus seedlings

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处理	根长/cm	断根强度	IBA浓度/（mg·L^-1）
F1	1.5~2.0	不断根	-
F2		断根尖0.5 cm	-
P1		断根尖0.5 cm	0.010
P2			0.025
P3			0.075
P4			0.125
S1	3.0~4.0	不断根	-
S2		断根尖0.5 cm	-
S3		断根长1/2	-
T1	4.5~6.0	不断根	-
T2		断根尖0.5 cm	-
T3		断根长1/2	-
T4		留根长2 cm	-

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