气象因子对高寒草地紫花针茅内生真菌共生体凋落物分解的影响

doi:10.13866/j.azr.2025.07.10

Abstract

Abstract:

Litter decomposition has an important role in the carbon and nutrient cycling of terrestrial ecosystems. Climatic conditions are the main factors involved in litter decomposition. Currently, few studies have examined litter decomposition in alpine grassland ecosystems. To determine the effects of meteorological factors on the litter decomposition and nutrient release processes under a climate change background, the Stipa purpurea endophytic fungal symbiont was used along with the litter decomposition bag method to analyze the decomposition characteristics with endophytic fungi (E+) and without endophytic fungi (E-). The effect of meteorological factors on the decomposition rate of Stipa purpurea was analyzed. The results indicated that the decomposition rate of E+ was higher compared with that of E-, whereas the decomposition cycle was shorter. With the extension of time, the total nitrogen content of Stipa purpurea showed an increasing trend, lignin content gradually changed from significantly higher in E+ to no significant difference between the two, and the cellulose content gradually changed from significantly lower in E+ compared with E- (P<0.05). Regardless of endophytic fungi, the litter weight and mass loss rate of Stipa purpurea were correlated with the mean monthly temperature and the mean ground temperature (P<0.05). Precipitation was positively correlated with the litter decomposition rate of Stipa purpurea, and the total nitrogen content of the litter was positively correlated with temperature and precipitation (P<0.05). The lignin and cellulose content were negatively correlated with temperature and precipitation. The duration of sunshine had a positive effect on the decomposition of litter, and the lignin, cellulose, and litter weight content were strongly correlated with sunshine duration. Overall, endophytic fungi accelerate the decomposition of Stipa purpurea litter. For E+ and E-, the effect of meteorological factors on the decomposition of Stipa purpurea litter was consistent.

Key words: Stipa purpurea, endophytic fungi, litter decomposition, meteorological factors, alpine grassland

GUO Qiang, WANG Yuqin, SONG Meiling. Effects of meteorological factors on the litter decomposition of the fungal endophyte Stipa purpurea symbiont in alpine grassland[J].Arid Zone Research, 2025, 42(7): 1269-1278.

Figures/Tables 6

Fig. 1

Tab. 1

Olson exponential model and decomposition rate of E+ and E- Stipa purpurea litters"

凋落物种类	回归方程	相关系数（R²）	分解速率（k）	$T 50 % / a$	$T 95 % / a$
E+	y=1.1024e^-0.869^t	0.970	0.869	0.80	3.45
E-	y=1.09698e^-0.66^t	0.963	0.66	1.05	4.54

Tab. 1

Fig. 2

Fig. 3

Tab. 2

Fig. 4

References 49

[1]	王静, 赵萌莉, Willms W, 等. 内蒙古典型草原及不同功能群生产力对凋落物添加的响应[J]. 植物生态学报, 2010, 34(8): 907-914. doi: 10.3773/j.issn.1005-264x.2010.08.003
	[Wang Jing, Zhao Mengli, Willms W, et al. Productivity responses of different functional groups to litter addition in typical grassland of Inner Mongolia[J]. Chinese Journal of Plant Ecology, 2010, 34(8): 907-914.] doi: 10.3773/j.issn.1005-264x.2010.08.003
[2]	王静, 张峰, 李治国, 等. 不同剂量凋落物添加对荒漠草原生物量的影响[J]. 中国草地学报, 2023, 45(6): 64-72.
	[Wang Jing, Zhang Feng, Li Zhiguo, et al. Effect of different doses of litter addition on the biomass of desert steppe[J]. Chinese Journal of Grassland, 2023, 45(6): 64-72.]
[3]	曲浩, 赵学勇, 赵哈林, 等. 科尔沁沙地3种灌木凋落物分解速率及其与关键气象因子的关系[J]. 中国沙漠, 2010, 30(4): 844-849.
	[Qu Hao, Zhao Xueyong, Zhao Halin, et al. Litter decomposition rates of three shrub species in Horqin Sandy Land and their relationship with key meteorological factors[J]. Journal of Desert Research, 2010, 30(4): 844-849.]
[4]	薛立, 邝立钢. 杉木凋落物分解速率的研究[J]. 四川林业科技, 1990, 11(1): 1-4.
	[Xue Li, Kuang Ligang. Study on decomposition rate of Chinese fir litter[J]. Journal of Sichuan Forestry Science and Technology, 1990, 11(1): 1-4.]
[5]	Couteaux M M, Bottner P, Berg B. Litter decomposition climate and litter quality[J]. Trends in Ecology and Evolution, 1995, 10(2): 63-66.
[6]	Swift M J, Heal O W, Anderson J M. Decomposition in terrestrial ecosystems[J]. Studies in Ecology, 1979, 5(14): 2772-2774.
[7]	Lucía V, Amy T A. The importance of macro-and micro-nutrients over climate for leaf litter decomposition and nutrient release in Patagonian temperate forests[J]. Forest Ecology and Management, 2019, 441: 144-154.
[8]	李凯, 赵文东, 朱传晟, 等. 滨海防护林月凋落物量及其与气象因子的关系[J]. 森林与环境学报, 2021, 41(6): 570-575.
	[Li Kai, Zhao Wendong, Zhu Chuansheng, et al. Monthly litter production of Acacia aulacocarpa and Pinus elliottii artificial forest and its relationship with meteorological factors[J]. Journal of Forest and Environment, 2021, 41(6): 570-575.]
[9]	Kitayamak , Aiba S. Ecosystem structure and productivity of tropical rain forests along altitudinal gradients with contrasting soil phosphorus pools on Mount Kinabalu, Borneo[J]. Journal of Ecology, 2002, 90(1): 37.
[10]	Liu G F, Wang L, Liang L, et al. Specific leaf area predicts dryland litter decomposition via two mechanisms[J]. Journal of Ecology, 2018, 106: 218-229.
[11]	邓秀秀, 王忠诚, 李程, 等. 浙江天童常绿阔叶林凋落物量季节动态及其与气象因子的关系[J]. 中南林业科技大学学报, 2017, 37(3): 73-78.
	[Deng Xiuxiu, Wang Zhongcheng, Li Cheng, et al. Seasonal dynamics of the litter fall production of evergreen broadleaf forest and its relationships with meteorological factors at Tiantong of Zhejiang Province[J]. Journal of Central South University of Forestry & Technology, 2017, 37(3): 73-78.]
[12]	宋梅玲, 王玉琴, 王宏生, 等. 内生真菌对高寒草地紫花针茅凋落物分解的影响[J]. 草业学报, 2021, 30(9): 150-158. doi: 10.11686/cyxb2020462
	[Song Meiling, Wang Yuqin, Wang Hongsheng, et al. Effect of Epichloë endophyte on the litter decomposition of Stipa purpurea in alpine grassland[J]. Acta Prataculturae Sinica, 2021, 30(9): 150-158.] doi: 10.11686/cyxb2020462
[13]	岳鹏鹏, 卢学峰, 叶润蓉, 等. 青海湖地区紫花针茅草原群落特征[J]. 草业学报, 2014, 23(4): 10-19. doi: 10.11686/cyxb20140402
	[Yue Pengpeng, Lu Xuefeng, Ye Runrong, et al. Community characteristies of Stipa purpurea stepp in the Qinghai Lake region[J]. Acta Prataculturae Sinica, 2014, 23(4): 10-19.] doi: 10.11686/cyxb20140402
[14]	张晓艳, 胡玉昆, 李凯辉, 等. 围封条件下紫花针茅群落主要结构特征和地上生物量变化[J]. 干旱区资源与环境, 2009, 23(1): 197-200.
	[Zhang Xiaoyan, Hu Yukun, Li Kaihui, et al. The changes of community structure and above ground biomass in Stipa purpurea steppe of enclosure[J]. Journal of Arid Land Resources and Environment, 2009, 23(1): 197-200.]
[15]	洪江涛, 吴建波, 王小丹. 放牧和围封对藏北高寒草原紫花针茅群落生物量分配及碳、氮、磷储量的影响[J]. 草业科学, 2015, 32(11): 1878-1886.
	[Hong Jiangtao, Wu Jianbo, Wang Xiaodan. Effects of grazing and fencing on Stipa purpurea community biomass allocation and carbon, nitrogen and phosphorus pools on the northern Xizang Plateau alpine[J]. Pratacultural Science, 2015, 32(11): 1878-1886.]
[16]	付莉娇, 李雪琴, 范继辉, 等. 藏北高寒草原典型植物根际土壤细菌群落结构多样性及根系特征分析[J]. 草地学报, 2022, 30(5): 1131-1140. doi: 10.11733/j.issn.1007-0435.2022.05.013
	[Fu Lijiao, Li Xueqin, Fan Jihui, et al. Analysis of rhizosphere soil bacterial community structure diversity and root characteristics of typical plants in alpine steppe of Northern Tibet[J]. Acta Agrestia Sinica, 2022, 30(5): 1131-1140.] doi: 10.11733/j.issn.1007-0435.2022.05.013
[17]	鲍根生. 甘肃马先蒿寄生对紫花针茅内生真菌共生体生长和光合特性的影响[D]. 兰州: 兰州大学, 2015.
	[Bao Gensheng. Effects of the Hemiparasitic Plant Pedicularis kansuensis on Growth and Photosynthetic Properties of Stipa purpurea-Epichloë symbiosis[D]. Lanzhou: Lanzhou University, 2015.]
[18]	Gundel P E, Helander M, Garibaldi L A, et al. Role of foliar fungal endophytes in litter decomposition among species and population origins[J]. Fungal Ecology, 2016, 21: 50-56.
[19]	李春杰, 南志标, 刘勇, 等. 醉马草内生真菌检测方法的研究[J]. 中国食用菌, 2008, 27 (Suppl.): 16-19.
	[Li Chunjie, Nan Zhibiao, Liu Yong, et al. Methodology of endophyte detection of drunken horse grass (Achnatherum inebrians)[J]. Edible Fungi of China, 2008, 27 (Suppl.): 16-19.]
[20]	黎冬容, 张世庆, 甘世端, 等. 全自动凯氏定氮仪测定土壤全氮含量[J]. 南方国土资源, 2015(8): 38-39.
	[Li Dongrong, Zhang Shiqing, Gan Shirui, et al. Determination of total nitrogen content in soil by automatic Kjeldahl nitrogen analyzer[J]. Southern Land Resources, 2015(8): 38-39.]
[21]	何忠武. 酸性洗涤纤维和木质素测定的国际标准[J]. 饲料广角, 2011(6): 31-32.
	[He Zhongwu. International standard for determination of acid detergent fibers and lignin[J]. Feed China, 2011(6): 31-32.]
[22]	李娜, 赵传燕, 郝虎, 等. 海拔和郁闭度对祁连山青海云杉林叶凋落物分解的影响[J]. 生态学报, 2021, 41(11): 4493-4502.
	[Li Na, Zhao Chuanyan, Hao Hu, et al. Decomposition and its nutients dyamic of Qinghai spruce leaf litter with elevation gradient in Qilian Mountains[J]. Acta Ecologica Sinica, 2021, 41(11): 4493-4502.]
[23]	Olson J S. Energy storage and the balance of producers and decomposers in ecological systems[J]. Ecology, 1963, 44: 322-331.
[24]	王嘉年, 李向义, 李成道, 等. 自然光照和荫蔽条件下两种荒漠植物叶片凋落物分解特征研究[J]. 干旱区地理, 2023, 46(6): 949-957. doi: 10.12118/j.issn.1000-6060.2022.434
	[Wang Jianian, Li Xiangyi, Li Chengdao, et al. Decomposition characteristics of two desert plant leaf under natural light and shade environment[J]. Arid Land Geography, 2023, 46(6): 949-957.] doi: 10.12118/j.issn.1000-6060.2022.434
[25]	张悦, 张艺凡, 马怡波, 等. 森林生态系统凋落物分解影响因素研究进展[J]. 环境生态学, 2023, 5(4): 45-56.
	[Zhang Yue, Zhang Yifan, Ma Yibo, et al. Research progress on influencing factors of litter decomposition in forest ecosystem[J]. Environmental Ecology, 2023, 5(4): 45-56.]
[26]	Uroz S, Buee M, Deveau A, et al. Ecology of the forest microbiome: Highlights of temperate and boreal ecosystems[J]. Soil Biology and Biochemistry, 2016, 103: 471-488.
[27]	Saikkonen K, Mikola J, Helander M. Endophytic phyllosphere fungi and nutrient cycling in terrestrial ecosystems[J]. Current Science, 2015, 109(1): 121-126.
[28]	侯卓男, 李欣彤, 张新军, 等. 海拔和坡向对色季拉山高山杜鹃凋落物分解的影响[J]. 中国农业大学学报, 2024, 29(4): 264-273.
	[Hou Zhuonan, Li Xintong, Zhang Xinjun, et al. Effects of elevation and slope orientation on litter decomposition of Rhododendron simsii in Mount Segrila[J]. Journal of China Agricultural University, 2024, 29(4): 264-273.]
[29]	Bell-Dereske L, Gao X D, Masiello C A, et al. Plant-fungal symbiosis affects litter decomposition during primary succession[J]. Oikos, 2017, 126(6): 801-811.
[30]	Andersson M, Kjøller A, Struwe S. Microbial enzyme activities in leaf litter, humus and mineral soil layers of European forests[J]. Soil Biology and Biochemistry, 2004, 36(10): 1527-1537.
[31]	Lemons A, Clay K, Rudgers J A. Connecting plant-microbial interactions above and belowground: A fungal endophyte affects decomposition[J]. Oecologia, 2005, 145(4): 595-604. pmid: 16001218
[32]	Jiang Y F, Yin X Q, Wang F B. The influence of litter mixing on decomposition and soil fauna assemblages in a Pinus koraiensis mixed broadleaved forest of the Changbai Mountains, China[J]. European Journal of Soil Biology, 2013, 55: 28-39.
[33]	宋学贵, 胡庭兴, 鲜骏仁, 等. 川西南常绿阔叶林凋落物分解及养分释放对模拟氮沉降的响应[J]. 应用生态学报, 2007, 18(10): 2167-2172.
	[Song Xuegui, Hu Tingxing, Xian Junren, et al. Responses of litter decom position and nutrient release to simulated nitrogen deposition m an evergreen broad-leaved forest in southwestern Sichuan[J]. Chinese Journal of Applied Ecology, 2007, 18(10): 2167-2172.] pmid: 18163293
[34]	Xu D W, Liu J F, Marshall P, et al. Leaf litter decomposition dynamics in unmanaged Phyllostachys pubescens stands at high elevations in the Daiyun Mountain national nature reserve[J]. Journal of Mountain Science, 2017, 14(11): 2246-2256.
[35]	Frey S, Elliott E, Paustian K, et al. Fungal translocation as a mechanism for soil nitrogen inputs to surface residue decomposition in a no-tillage agroecosystem[J]. Soil Biology and Biochemistry, 2000, 32(5): 689-698.
[36]	彭少麟, 刘强. 森林凋落物动态及其对全球变暖的响应[J]. 生态学报, 2002, 22(9): 1534-1544.
	[Peng Shaolin, Liu Qiang. The dynamics of forest litter and its responses to global warming[J]. Acta Ecologica Sinica, 2002, 22(9): 1534-1544.]
[37]	Liu G D, Sun J F, Tian K, et al. Long-term responses of leaf litter decomposition to temperature, liter quality and litter mixing in plateau wetlands[J]. Freshwater Biology, 2017, 62(1): 178-190.
[38]	周梦田, 刘莉, 付若仙, 等. 杉木与木荷凋落物分解对杉木人工林土壤碳氮含量和酶活性的影响[J]. 南京林业大学学报(自然科学版), 2024, 48(5): 131-138. doi: 10.12302/j.issn.1000-2006.202304024
	[Zhou Mengtian, Liu Li, Fu Ruoxian, et al. Effects of litter decomposition of Cunninghamia lanceolata and Schima superba on soil carbon contents, nitrogen contents and enzyme activities in Cunninghamia lanceolata plantations[J]. Journal of Nanjing Forestry University (Natural Sciences Edition), 2024, 48(5): 131-138.]
[39]	熊红福. 贵州省喀斯特地区凋落物分解对气候变暖的响应[J]. 现代农业科技, 2023(24): 118-121, 127.
	[Xiong Hongfu. Litter decomposition and its responses to climate warming in Karst area of Guizhou Province[J]. Modern Agricultural Science and Technology, 2023(24): 118-121, 127.]
[40]	Kravchenko I K, Tikhonova E N, Ulanova R V, et al. Effect of temperature on litter decomposition, soil microbial community structure and biomass in a mixed-wood forest in European Russia[J]. Current Science, 2019, 116(5): 765-772. doi: 10.18520/cs/v116/i5/765-772
[41]	代松家, 周晨霓, 段斐, 等. 组分和生境差异对藏东南原始冷杉林凋落物分解和养分释放特征的影响[J]. 中国水土保持科学, 2020, 18(6): 72-80.
	[Dai Sunjia, Zhou Chenni, Duan Fei, et al. Effects of composition and habitat differences on litter decomposition and nutrient release characteristics of Abies georgei var. smithii in southeastern Xizang[J]. Science of Soil and Water Conservation, 2020, 18(6): 72-80.]
[42]	霍利霞, 红梅, 赵巴音那木拉, 等. 氮沉降和降雨变化对荒漠草原凋落物分解的影响[J]. 生态学报, 2019, 39(6): 2139-2146.
	[Huo Lixia, Hong Mei, Zhao Bayinnamula, et al. Effects of increased nitrogen deposition and changing rainfall patterns on litter decomposition in a desert grassland[J]. Acta Ecologica Sinica, 2019, 39(6): 2139-2146.]
[43]	Salamanca E F, Kaneko N, Katagiri S. Rainfall manipulation effects on litter decomposition and the microbial biomass of the forest floor[J]. Applied Soil Ecology, 2003, 22(3): 271-281.
[44]	王新源, 赵学勇, 李玉霖, 等. 环境因素对干旱半干旱区凋落物分解的影响研究进展[J]. 应用生态学报, 2013, 24(11): 3300-3310.
	[Wang Xinyuan, Zhao Xueyong, Li Yulin, et al. Effects of environmental factors on litter decomposition in arid and semi-arid regions: A review[J]. Chinese Journal of Applied Ecology, 2013, 24(11): 3300-3310.] pmid: 24564163
[45]	郑俊强, 郭瑞红, 李东升, 等. 氮沉降和干旱对阔叶红松林凋落物分解的影响[J]. 北京林业大学学报, 2016, 38(4): 21-28.
	[Zhen Junqiang, Guo Ruihong, Li Dongsheng, et al. Effects of nitrogen deposition and drought on litter decomposition in a temperate forest[J]. Journal of Beijing Forestry University, 2016, 38(4): 21-28.]
[46]	Kurzatkowski D, Martius C, Höfer H, et al. Litter decomposition, microbial biomass and activity of soil organisms in three agroforestry sites in Central Amazonia[J]. Nutrient Cycling in Agroecosystems, 2004, 69(3): 257-267.
[47]	Day T A, Guenonr, Ruhland C T. Photodegradation of plant litter in the Sonoran Desert varies by litter type and age[J]. Soil Biology and Biochemistry, 2015, 89: 109-122.
[48]	Baker N R, Allison S D. Ultraviolet photodegradation facilitates microbial litter decomposition in a Mediterranean climate[J]. Ecology, 2015, 96: 1994-2003. pmid: 26378321
[49]	He M, Zhao R D, Tian Q X, et al. Predominant effects of litter chemistry on lignin degradation in the early stage of leaf litter decomposition[J]. Plant Soil, 2019, 442: 453-469.

凋落物种类	指标	月平均气温	降水量	月平均地面温度	日照时数
E+	凋落物重量	-0.990^**	-0.802	-0.993^**	0.290
	质量损失率	0.989^**	0.805	0.992^**	-0.287
	全氮	0.872^*	0.915^*	0.856^*	-0.502
	木质素	-0.938^**	-0.659	-0.955^**	0.169
	纤维素	-0.924^**	-0.703	-0.935^**	0.065
E-	凋落物重量	-0.995^**	-0.831^*	-0.992^**	0.369
	质量损失率	0.993^**	0.835^*	0.989^**	-0.367
	全氮	0.931^**	0.885^*	0.917^*	-0.398
	木质素	-0.894^*	-0.640	-0.910^*	0.033
	纤维素	-0.913^*	-0.710	-0.921^**	0.063

Effects of meteorological factors on the litter decomposition of the fungal endophyte Stipa purpurea symbiont in alpine grassland

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