G.przewalskii; natural drought,osmotic regulation substance,antioxidant enzyme activity,Gansu Province

  ,"/> Response of Osmotic Regulation Substances and Antioxidant Enzyme Activity in Leaves of<em> Gymnocarpos przewalskii</em> to Drought

Arid Zone Research ›› 2020, Vol. 37 ›› Issue (1): 227-235.

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Response of Osmotic Regulation Substances and Antioxidant Enzyme Activity in Leaves of Gymnocarpos przewalskii to Drought

HUANG Hai-xia1, LIAN Zhuan-hong1, WANG Liang2, YANG Qi-qi1, WEI Zhen-yan1, MA Yan-jun1, ZHANG Jin-xia3   

  1. 1. College of Forestry, Gansu Agricultural University, Lanzhou 730070,Gansu,China;
    2. Administration of Gansu Anxi Extra-arid Desert National Nature Reserve, Jiuquan 736100, Gansu,China;
    3. College of Water Conservancy and Hydropower Engineering, Gansu Agricultural University, Lanzhou 730070,Gansu,China

  • Received:2019-03-01 Revised:2019-05-06 Online:2020-01-15 Published:2020-01-15

Abstract: As a relict species of tertiary in desert area, Gymnocarpos przewalskii originates from the Tethys. In this study, the leaves of G. przewalskii were collected from the medium- and old-aged individuals in the Anxi Nature Reserve in Gansu Province in June, July and August 2017. The samples were used to analyze the change of osmotic regulation substances and antioxidant enzyme activity under natural drought. The results showed that the soluble protein (SP) accumulated significantly with the increase of drought level and played the main osmotic regulation, the soluble sugar (SS) content in leaves of the medium-aged individuals and the proline (Pro) content in those of the old-aged ones increased at first and then decreased obviously, and the osmotic regulation was limited. As for the medium-aged individuals, when the drought level was increased, the superoxide dismutase (SOD) activity and ascorbate peroxidase (APX) activity significantly increased at first and then decreased, and the peroxidase (POD) activity and catalase (CAT) activity were increased significantly under severe drought, which indicated that the four enzymes scavenged the active oxygen by synergistic effect. For the old-aged ones, the CAT activity was continuously increased, the APX activity was obviously increased at first and then decreased, and the POD activity was significantly increased under severe drought, which showed that the damage to membrane system was mainly relieved by the three enzymes. The malondialdehyde (MDA) content in leaves was significantly increased with the increase of drought level and membrane lipid peroxidation. Under the situation with similar rainfall, there were the significant differences between the medium- and the old-aged individuals in the response of Pro, SS and SP content, POD and CAT activity and MDA content in leaves to drought. The result of subordinate function method showed that the drought resistance of the old-aged individuals was higher than that of the medium-aged ones, indicating the drought resistance of G.przewalskii would enhance with the age increase.

Key words: G.przewalskii; natural drought')">

G.przewalskii; natural drought, osmotic regulation substance, antioxidant enzyme activity,   ')">Gansu Province