Abstract: In greenhouse,two kinds of organic fertilizer including chicken manure and vermicompost and biochar were applied,and drip irrigation and flood irrigation were used so as to research the effects of different fertilization and irrigation modes on soil carbon content and soil enzyme activity. The results are as follows: ① fertilization of organic fertilizers could improve the activity of soil urease,phosphatase and catalase,however,the soil cellulose activity could be increased by 34.6% only with the Q+C+D treatment,and it was decreased by other treatments. Soil urease activity was increased by applying biochar,but the effects of biochar on other enzymes were different. Soil urease activity under the J+C+D treatment was increased by 40.6% and the phosphatase activity under the J+D treatment was increased by 20.3% due to the application of drip irrigation. There was no significant effect of drip irrigation on other treatments; ② Fertilization of organic fertilizers increased the content of soil nitrate nitrogen,in which the content of soil nitrate nitrogen under the J+C+D treatment was the highest (50.5 mg·kg^-1). Drip irrigation also increased significantly the content of soil nitrate nitrogen under the same fertilization treatments; ③ The contents of soil TC,TOC,WSTC and WSOC were increased by 5.0%-36.3%,11.8%-63.9%,5.2%-48.8% and 5.6%-46.5% respectively comparing with CK due to the fertilization of organic manure. The contents of soil TC,TOC,WSTC and WSOC were also increased by applying biochar. Flood irrigation decreased significantly the contents of soil TC,TOC,WSTC and WSOC under J+C+D treatment; ④ There was the significant positive correlation between soil soluble total carbon content and soil urease,catalase and cellulose activity,and only a significant correlation between soil soluble organic carbon content and soil urease activity. There was a significant negative correlation between soil inorganic carbon content and phosphatase and cellulose activity.

Key words: organic fertilizer, biochar, irrigation, soil carbon, soil enzyme, enzyme activity