污泥热碱液对干旱胁迫下小青菜生长的缓解机制

doi:10.13866/j.azr.2024.01.08

干旱区研究 ›› 2024, Vol. 41 ›› Issue (1): 80-91.doi: 10.13866/j.azr.2024.01.08 cstr: 32277.14.j.azr.2024.01.08

污泥热碱液对干旱胁迫下小青菜生长的缓解机制

白炬^1,²(),刘晓林^1,^2,³,李申^1,²,梁哲铭^1,²,胥子航^1,²,王永亮^1,²,杨治平^1,²()

1.山西农业大学生态环境产业技术研究院，农业农村部盐碱土改良与利用重点实验室（学科群），山西太原 030031
2.土壤环境与养分资源山西省重点实验室，山西太原 030031
3.山西大学生命科学学院，山西太原 030006

收稿日期:2023-07-20 修回日期:2023-10-10 出版日期:2024-01-15 发布日期:2024-01-24
作者简介:白炬（1994-），男，博士，助理研究员，主要从事作物施肥与新型肥料研发研究. E-mail: baiju@sxau.edu.cn
基金资助:
山西省科技重大专项计划项目(202201140601028);山西省重点研发计划项目(202202140601010);山西农业大学“特”“优”农业高质量发展科技支撑工程(TYGC-34);山西农业大学校企合作项目(2023HX006)

Mechanism of sludge alkaline thermal hydrolysis liquid on the growth of Brassica chinensis under drought stress

BAI Ju^1,²(),LIU Xiaolin^1,^2,³,LI Shen^1,²,LIANG Zheming^1,²,XU Zihang^1,²,WANG Yongliang^1,²,YANG Zhiping^1,²()

1. Key Laboratory of Saline-Alkali Soil Improvement and Utilization, Ministry of Agriculture and Rural Affairs (Subject Group), Institute of Eco-environment and Industrial Technology, Shanxi Agricultural University, Taiyuan 030031, Shanxi, China
2. Shanxi Provincial Key Laboratory of Soil Environment and Nutrient Resources, Taiyuan 030031, Shanxi, China
3. College of Life Sciences, Shanxi University, Taiyuan 030006, Shanxi, China

Received:2023-07-20 Revised:2023-10-10 Published:2024-01-15 Online:2024-01-24

摘要/Abstract

摘要：

近年来，为实现城市生活污泥的资源化利用，通过碱热水解技术制成的污泥热碱液被广泛关注。干旱是自然界中主要非生物胁迫之一，严重限制了作物的生长发育。为了改善干旱胁迫对蔬菜作物的影响，同时实现污泥资源化利用，本研究以小青菜（Brassica chinensis）为材料，通过盆栽试验设置4个水分梯度模拟不同干旱程度，在不同干旱程度下分别冲施等氮量的热碱液和尿素溶液，探讨热碱液对叶菜抵抗干旱胁迫的作用机制。结果表明：干旱胁迫下，经热碱液处理后，小青菜根长密度、根表面积密度和根体积密度均显著增加，进而促进对水分和氮素营养的吸收，提高叶片相对含水量，维持光合作用；冗余分析表明热碱液的施用主要提高过氧化氢酶活性和叶片相对含水量降低干旱胁迫对小青菜生长的负面影响。综上所述，将污泥热碱液应用到蔬菜生产中可缓解干旱胁迫对叶菜造成的生长抑制，为干旱地区蔬菜生产提供了策略，也为有效无害化、资源化利用城市生活污泥提供了一种新途径。

关键词: 污泥热碱液, 干旱胁迫, 抗氧化酶活性, 冗余分析, 污泥资源化利用

Abstract:

In recent years, there has been considerable attention given to the utilization of urban domestic sludge through the production of sludge alkaline thermal hydrolysis liquid using alkaline hot water lysis. Drought is a major abiotic stress in nature, severely limiting crop production. To effectively mitigate the adverse impact of drought stress on vegetables and explore the potential of sludge utilization, this study used Brassica chinensis as the research object. Different levels of drought stress were induced by creating four soil moisture gradients. Equal amounts of nitrogen from alkaline thermal hydrolysis liquid derived from sludge and urea solution were applied under different levels of drought stress to examine the resistance mechanism of the sludge-derived alkaline thermal hydrolysis liquid to drought stress. The results showed that when exposed to different drought stress conditions, the use of alkaline thermal hydrolysis liquid derived from sludge significantly improved the root length density, root surface area density, and root volume density. This enhancement improved the ability of B. chinensis to absorb water and nutrients from the soil and increased the leaf’s relative water content, helping maintain the plant’s photosynthesis process. Based on the redundancy analysis, applying alkaline thermal hydrolysis liquid to sludge could increase the leaf’s relative water content and improve catalase activity, thereby alleviating the growth inhibition of B. chinensis induced by drought stress. In summary, the application of alkaline thermal hydrolysis liquid in vegetable production can help alleviate growth impediments caused by drought stress on leafy vegetables, provide a strategy for vegetable production in arid areas, and introduce a novel method for the safe and effective utilization of urban sludge.

Key words: alkaline thermal hydrolysis liquid of sludge, drought stress, autioxidant enzyme activities, redundancy analysis, sludge resource utilization

白炬, 刘晓林, 李申, 梁哲铭, 胥子航, 王永亮, 杨治平. 污泥热碱液对干旱胁迫下小青菜生长的缓解机制[J]. 干旱区研究, 2024, 41(1): 80-91.

BAI Ju, LIU Xiaolin, LI Shen, LIANG Zheming, XU Zihang, WANG Yongliang, YANG Zhiping. Mechanism of sludge alkaline thermal hydrolysis liquid on the growth of Brassica chinensis under drought stress[J]. Arid Zone Research, 2024, 41(1): 80-91.

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因子	含量	因子	含量
钙/（g·L^-1）	55.3±3.7	砷/（mg·L^-1）	1.8±0.1
全氮/（g·kg^-1）	40.0±0.2	铅/（mg·L^-1）	3.0±0.1
多肽/（g·L^-1）	116.7±12.1	铬/（mg·L^-1）	1.8±0.1
蛋白质/（g·L^-1）	35.7±1.6	汞/（μg·L^-1）	66.2±5.9
游离氨基酸/（g·L^-1）	54.7±5.2	镉/（μg·L^-1）	12.3±3.8

逆境相关指标	轻度胁迫（LD）			中度胁迫（MD）			重度胁迫（SD）
逆境相关指标	Explains/%	F	P	Explain/%	F	P	Explain/%	F	P
CAT	61.8	6.5	0.008	52.5	4.4	0.006	3.6	<0.1	1
MDA	10	1.1	0.442	18	1.8	0.17	7.6	1.7	0.23
POD	12.8	1.7	0.256	8.9	1.6	0.398	-	-	-
SS	11.6	3.1	0.224	-	-	-	2.4	0.7	0.56
SOD	3.8	<0.1	1	-	-	-	-	-	-
Pro	-	-	-	14.9	2	0.158	7.7	2.6	0.14
RWC	-	-	-	5.7	<0.1	1	78.8	14.8	0.04

污泥热碱液对干旱胁迫下小青菜生长的缓解机制

Mechanism of sludge alkaline thermal hydrolysis liquid on the growth of Brassica chinensis under drought stress

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