干旱区研究

Arid Zone Research >

2021 , Vol. 38 >Issue 6: 1713 - 1721

DOI: https://doi.org/10.13866/j.azr.2021.06.23

Plant and Plant Physiology

Effect of water stress on sugar accumulation and sucrose metabolism enzyme activities of greenhouse grape fruit

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  • College of Water Conservancy and Hydropower Engineering, Gansu Agricultural University, Lanzhou 730070, Gansu, China

Received date: 2021-01-04

  Revised date: 2021-06-15

  Online published: 2021-11-29

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Abstract

To analyze the effects of water stress on fruit sugar components and related metabolic enzyme activity, the greenhouse table grape Red Globe was used as test material in four water treatment field trials. The results showed that sugar accumulation of Red Globe grape fruit is dominated by hexose (fructose and glucose); when the fruit matures, the content of various sugars is highest under mild stress and lowest under severe stress. Sufficient water is supplied in the early period of fruit expansion and at the end of the expansion period. Mild stress during ripening and coloring periods helps accumulate effective sugars in the fruit. Mild stress promotes activity and quality of sucrose metabolizing enzymes in grape fruit to increase yield by 12.15%, while severe stress shows strong inhibition. Output levels reduced by 18.6%. Fruit sugar metabolism results from combined effects of related enzyme activities, which is indirectly regulated by water stress by affecting enzyme activities.

Key words： water stress; greenhouse grape; sugar accumulation; enzyme activity; fruit quality

Cite this article

GAO Yanting,ZHANG Rui,LI Hongxia,WEI Pengcheng . Effect of water stress on sugar accumulation and sucrose metabolism enzyme activities of greenhouse grape fruit[J]. Arid Zone Research, 2021 , 38(6) : 1713 -1721 . DOI: 10.13866/j.azr.2021.06.23

References

[1] 何钊全, 张铜会, 刘新平, 等. 甘肃河西干旱区温室葡萄对地表覆盖的响应[J]. 中国沙漠, 2018, 38(5):1049-1058.
[1] [ He Zhaoquan, Zhang Tonghui, Liu Xinping, et al. Response of greenhouse grape to ground cover in Hexi Arid Area of Gansu[J]. Journal of Desert Research, 2018, 38(5):1049-1058. ]
[2] 钟海霞, 潘明启, 张付春, 等. 不同砧木对克瑞森葡萄果实可溶性糖含量的影响[J]. 新疆农业科学, 2018, 55(9):1633-1638.
[2] [ Zhong Haixia, Pan Mingqi, Zhang Fuchun, et al. Effects of different rootstocks on the soluble sugar content of Cresen grape fruit[J]. Xinjiang Agricultural Sciences, 2018, 55(9):1633-1638. ]
[3] 赵艳侠, 亓桂梅, 董兴全, 等. 葡萄果实中糖代谢及转运机制研究进展[J]. 中国果树, 2019, 61(5):7-11.
[3] [ Zhao Yanxia, Qi Guimei, Dong Xingquan, et al. Research progress on sugar metabolism and transport mechanism in grape fruit[J]. China Fruits, 2019, 61(5):7-11. ]
[4] 赵建华, 李浩霞, 安巍, 等. 干旱胁迫对宁夏枸杞叶片蔗糖代谢及光合特性的影响[J]. 西北植物学报, 2013, 33(5):970-975.
[4] [ Zhao Jianhua, Li Haoxia, An Wei, et al. Effects of drought stress on sucrose metabolism and photosynthetic characteristics of Lycium barbarum leaves in Ningxia[J]. Acta Botanica Boreali-Occidentalia Sinica, 2013, 33(5):970-975. ]
[5] 张上隆, 陈昆松. 果实品质形成与调控的分子生理[M]. 北京: 中国农业出版社, 2007.
[5] [ Zhang Shanglong, Chen Kunsong. Molecular Physiology of Fruit Quality Formation and Regulation[M]. Beijing: China Agriculture Press, 2007. ]
[6] 单长卷, 韩蕊莲, 梁宗锁. 干旱胁迫下黄土高原4种乡土禾草抗氧化特性[J]. 生态学报, 2012, 32(4):170-180.
[6] [ Shan Changjuan, Han Ruilian, Lang Zongsuo. Antioxidant characteristics of 4 native grasses in the Loess Plateau under drought stress[J]. Acta Ecologica Sinica, 2012, 32(4):170-180. ]
[7] 李昭楠, 李唯, 刘继亮, 等. 不同滴灌水量对干旱荒漠区酿酒葡萄光合及产量的影响[J]. 中国生态农业学报, 2011, 19(6):1324-1329.
[7] [ Li Zhaonan, Li Wei, Liu Jiliang, et al. Effect of drip irrigation pattern on wine grape growth, yield, photosynjournal and water use efficiency in arid desert regions[J]. Chinese Journal of Eco-Agriculture, 2011, 19(6):1324-1329. ]
[8] 刘军, 齐广平, 康燕霞, 等. 土壤水分胁迫对紫花苜蓿光合特性及其生物量的影响[J]. 干旱区研究, 2019, 36(4):893-900.
[8] [ Liu Jun, Qi Guangping, Kang Yanxia, et al. Effects of soil water stress on photosynthetic characteristics and biomass of Medicago sativa[J]. Arid Zone Research, 2019, 36(4):893-900. ]
[9] 杜太生, 康绍忠, 张霁, 等. 不同沟灌模式对沙漠绿洲区葡萄生长和水分利用的效应[J]. 应用生态学报, 2006, 17(5):805-810.
[9] [ Du Taisheng, Kang Shaozhong, Zhang Ji, et al. Effects of different furrow irrigation modes on grape growth and water use in desert oasis[J]. Chinese Journal of Applied Ecology, 2006, 17(5):805-810. ]
[10] 邓浩亮, 孔维萍, 张恒嘉, 等. 不同生育期调亏灌溉对酿酒葡萄耗水及果实品质的影响[J]. 中国生态农业学报, 2016, 24(9):1196-1205.
[10] [ Deng Haoliang, Kong Weiping, Zhang Hengjia, et al. Effects of regulated deficit irrigation in different growth periods on water consumption and fruit quality of wine grapes[J]. Chinese Journal of Eco-Agriculture, 2016, 24(9):1196-1205. ]
[11] 刘静霞, 张芮, 成自勇, 等. 不同生育期水分亏缺对酿酒葡萄产量及品质的效应研究[J]. 干旱地区农业研究, 2016, 34(3):78-83.
[11] [ Liu Jingxia, Zhang Rui, Cheng Ziyong, et al. Effects of water deficit in different growth periods on the yield and quality of wine grapes[J]. Agricultural Research in the Arid Areas, 2016, 34(3):78-83. ]
[12] Carla Pinheiro, Maria Manuela Chaves, Cândido Pinto Ricardo. Alterations in carbon and nitrogen metabolism induced by water deficit in the stems and leaves of Lupinus albus L.[J]. Journal of Experimental Botany, 2001, 52(358):1063-1070.
[13] 龙友华, 张承, 吴小毛, 等. 10个猕猴桃品种在贵州主产区的引种表现[J]. 贵州农业科学, 2015, 43(7):5-8.
[13] [ Long Youhua, Zhang Cheng, Wu Xiaomao, et al. Introduction performance of ten kiwifruit varieties in the major production area of Guizhou[J]. Guizhou Agricultural Sciences, 2015, 43(7):5-8. ]
[14] 郭燕. 几个苹果品种果实糖酸积累及糖代谢相关酶活性变化研究[D]. 杨凌: 西北农林科技大学, 2012.
[14] [ Guo Yan. Study on Accumulation of Sugar and Acid in Fruits of Several Apple Cultivars[D]. Yangling: Northwest A&F University, 2012. ]
[15] 许传强, 李天来, 齐红岩. 嫁接对网纹甜瓜果实发育、糖含量及蔗糖代谢相关酶活性的影响[J]. 园艺学报, 2006, 33(4):773-778.
[15] [ Xu Chuanqiang, Li Tianlai, Qi Hongyan. Effects of grafting on fruit development, sugar content and sucrose metabolism related enzyme activities of netted muskmelon[J]. Acta Horticulturae Sinica, 2006, 33(4):773-778. ]
[16] 赵丹丹, 王俊, 付鑫. 长期定位施肥对旱作农田土壤有机碳及其组分的影响[J]. 干旱地区农业研究, 2017, 35(1):97-102.
[16] [ Zhao Dandan, Wang Jun, Fu Xin. Effects of long-term located fertilization on soil organic carbon and its components in dry farmland[J]. Agricultural Research in the Arid Areas, 2017, 35(1):97-102. ]
[17] Davies C, Robinson S P. Sugar accumulation in grape berries. Cloning of two putative vacuolar invertase cDNAs and their expression in grapevine tissues[J]. Plant Physiology, 1996, 111(1):275-283.
[18] 张芮, 成自勇, 王旺田, 等. 水分胁迫对延后栽培葡萄果实生长的影响[J]. 华南农业大学学报, 2015, 36(6):47-54.
[18] [ Zhang Rui, Cheng Ziyong, Wang Wangtian, et al. The effect of water stress on the growth of delayed grape cultivation[J]. Journal of South China Agricultural University, 2015, 36(6):47-54. ]
[19] 申孝军, 陈红梅, 孙景生, 等. 调亏灌溉对膜下滴灌棉花生长、产量及水分利用效率的影响[J]. 灌溉排水学报, 2010, 29(1):40-43.
[19] [ Shen Xiaojun, Chen Hongmei, Sun Jingsheng, et al. Effects of regulated deficit irrigation on the growth, yield and water use efficiency of cotton under mulch drip irrigation[J]. Journal of Irrigation and Drainage, 2010, 29(1):40-43. ]
[20] 熊江, 卢晓鹏, 李静, 等. 水分胁迫对果实品质的影响研究进展[J]. 湖南农业科学, 2014, 24(18):56-60.
[20] [ Xiong Jiang, Lu Xiaopeng, Li Jing, et al. Research progress on the effect of water stress on fruit quality[J]. Hunan Agricultural Sciences, 2014, 24(18):56-60. ]
[21] 谢兆森, 王世平, 许文平. 葡萄果实中的糖分积累和调控[J]. 植物生理学报, 2008, 58(4):785-790.
[21] [ Xie Zhaoseng, Wang Shiping, Xu Wenping. Sugar accumulation and regulation in grape fruit[J]. Plant Physiology Journal, 2008, 58(4):785-790. ]
[22] 李利梅, 王秀芹, 杨培培, 等. 赤霞珠葡萄果实糖积累与糖代谢相关酶的关系[J]. 中外葡萄与葡萄酒, 2011, 36(7):24-27.
[22] [ Li Limei, Wang Xiuqin, Yang Peipei, et al. The relationship between sugar accumulation and enzymes related to sugar metabolism in cabernet sauvignon grape[J]. Sino-Overseas Grapevine & Wine, 2011, 36(7):24-27. ]
[23] 闫梅玲, 代红军, 单守明, 等. 蔗糖代谢相关酶对果实糖积累影响的研究进展[J]. 安徽农业科学, 2009, 37(29):14021-14023, 14027.
[23] [ Yan Meiling, Dai Hongjun, Shan Shouming, et al. Research progress of the influence of sucrose-metabolizing enzymes on the sugar accumulation in fruit[J]. Journal of Anhui Agricultural Sciences, 2009, 37(29):14021-14023, 14027. ]
[24] Hubbard N L, Pharr D M, Huber S C. Sucrose phosphate synthase and other sucrose metabolizing enzymes in fruits of various species[J]. Physiologia Plantarum, 2010, 82(2):191-196.
[25] 贾名波, 肖秋红, 曹耀鹏, 等. 保水剂吸附尿素对‘赤霞珠’葡萄果实糖积累及蔗糖代谢酶活性的影响[J]. 中外葡萄与葡萄酒, 2018, 43(2):26-31.
[25] [ Jia Mingbo, Xiao Qiuhong, Cao Yaopeng, et al. Effect of super absorbent polymer and urea on sugar accumulation and sucrose metabolic enzyme activity of‘Cabernet Sauvignon’[J]. Sino-Overseas Grapevine & Wine, 2018, 43(2):26-31. ]
[26] Zhu S M, Liang Y L, An X J, et al. Changes in sugar co tent and related enzyme activities in table grape (Vitis vinifera L. ) in response to foliar selenium fertilizer[J]. Journal of the Science of Food & Agriculture, 2017, 97(12):4049-4102.
[27] 李鹏程, 郁松林, 符小发, 等. GA3对葡萄果实糖积累及蔗糖代谢酶的影响[J]. 西北农林科技大学学报(自然科学版), 2011, 39(10):177-183.
[27] [ Li Pengcheng, Yu Songlin, Fu Xiaofa, et al. Effects of GA3 on the fruit sugar accumulation and related enzyme activities in sugar metabolism of grape.[J]. Journal of Northwest A & F University (Natural Science Edition), 2011, 39(10):177-183. ]
[28] Zhang X Y, Wang X L, Wang X F, et al. A shift of phloem unloading from symplasmic to apoplasmic pathway is in-volved in developmental onset of ripening in grape berry[J]. Plant Physiology, 2006, 142(1):220-232.
[29] Hockema B R, Etxeberria E. Metabolic contributors to drought-enhanced accumulation of sugars and acids in oranges[J]. Journal of the American Society for Horticultural Science, 2001, 126(5):599-605.
[30] 刘翔宇, 邱燕萍, 陈杰忠, 等. 水分胁迫对‘桂味’荔枝果实发育及糖代谢的影响[J]. 果树学报, 2012, 29(4):620-624.
[30] [ Liu Xiangyu, Qiu Yanping, Chen Jiezhong, et al. Effect of water stress on litchi fruit development and sugar metabolism[J]. Journal of Fruit Science, 2012, 29(4):620-624. ]
[31] 罗霄, 郑国琦, 王俊. 果实糖代谢及其影响因素的研究进展[J]. 农业科学研究, 2008, 29(2):69-74.
[31] [ Luo Xiao, Zheng Guoqi, Wang Jun. Advances in research on sugar metabolism and its influencing factor in fruits[J]. Journal of Agricultural Sciences, 2008, 29(2):69-74. ]
[32] 赵智中, 陈俊伟, 张上隆, 等. 蔗糖代谢相关酶在温州蜜柑果实糖积累中的作用[J]. 园艺学报, 2001, 28(2):112-118.
[32] [ Zhao Zhizhong, Chen Junwei, Zhang Shanglong, et al. Roles of sucrose-metabolizing enzymes in accumulation of sugars in satsuma mandarin fruit[J]. Acta Horticulturae Sinica, 2001, 28(2):112-118. ]
[33] 张秋英, 刘晓冰, 金剑, 等. R5期遮阴对大豆植株体内源激素和酶活性的影响[J]. 大豆科学, 2004, 19(4):362-366.
[33] [ Zhang Qiuying, Liu Xiaobing, Jin Jian, et al. Effects of R5 shading on hormone and enzyme activities in soybean[J]. Soybean Science, 2004, 19(4):362-366. ]
[34] 夏国海, 张大鹏, 贾文锁. IAA、AA、GA和ABA对葡萄果实 14C蔗糖输入与代谢的调控[J]. 园艺学报, 2000, 39(1):6-10.
[34] [ Xia Guohai, Zhang Dapeng, Jia Wensuo. Effects of IAA, AA, GA and ABA on 14C-sucrose import and metabolism in grape berries[J]. Acta Horticulturae Sinica, 2000, 39(1):6-10. ]
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