Effects of water stress on rhizosphere organic nitrogen fractions and enzyme activities in the rhizosphere of delayed cultivation grape
Received date: 2020-12-23
Revised date: 2021-02-21
Online published: 2021-09-24
In order to explore the changes in the basic physical and chemical properties, enzyme activities and organic nitrogen components of the rhizosphere soil of protected grapes under different water stress conditions, and analyze the response relationship between them provide a certain reference basis for the establishment of a reasonable stress irrigation model for the delayed cultivation of grapes in the Hexi area of Gansu Province. Using a completely randomized test design based on a single water stress factor, the Bremner method was employed to determine the characteristics of the variation in organic nitrogen contents in the rhizosphere soil layer of the grapes during the ripening period, and the basic physical and chemical properties of the soil and soil enzyme activity during the whole growth period, under different water stresses (moderate W1, mild W2, and adequate W3 water supply). The results showed: (1) Moderate and mild water stress during the whole growth period had no significant effect on the total nitrogen, ammonium nitrogen, total phosphorus, organic matter, and organic carbon of the rhizosphere soil of the grapes under delayed cultivation, but the soil microbial biomass carbonand nitrogen contents were reduced. The contents of soil nitrate nitrogen and available phosphorus in the early stage of water stress were significantly lower than those observed in adequate water supply conditions. (2) Moderate and mild water stress had no significant effect on the activity of catalase in rhizosphere soil during the whole grape growth period, but showed different levels of inhibitory effects on the contents of urease and invertase in the fruit swelling and coloring stages. (3) The contents of organic nitrogen components in each treatment were present in the following order: non-acid hydrolysis nitrogen>acid hydrolysis amino acid nitrogen>acid hydrolysis ammonium nitrogen>acid hydrolysis unknown nitrogen>acid hydrolysis amino sugar nitrogen; both mild and moderate water stress contributed to the accumulation of total acid hydrolyzed nitrogen in the grapes’ rhizosphere soil, which increased by 21.16% and 10.34%, respectively, compared with contrast. (4) Sucrase had a significant and positive correlation with microbial biomass carbon; RDA analysis showed that nitrate nitrogen and ammonium nitrogen were the most important environmental factors affecting the variation of soil organic nitrogen components, followed by total nitrogen.
YANG Changyu,ZHANG Rui,LIN Baojun,DONG Bo,GAO Yanting,LI Hongxia,ZHAGN Caixia,WANG Xihong . Effects of water stress on rhizosphere organic nitrogen fractions and enzyme activities in the rhizosphere of delayed cultivation grape[J]. Arid Zone Research, 2021 , 38(5) : 1376 -1384 . DOI: 10.13866/j.azr.2021.05.19
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