Abstract: Two grape varieties (Gold Finger and Campbell) were taken as the materials to carry out a sand culture experiment,study the different expressions of genes of roots and leaves under various MnSO₄ stresses,and explore the differences in varieties and tissue in gene expression. It was found that,under the excess Mn stress,the up-expression folds of Vitis vinifera brassinosteroid-6-oxidase gene in the roots of Gold Finger were 2,and there was no significant change for other genes. In the leaves,the up-expression folds of V.vinifera copper chaperone for superoxide dismutase-like gene and V.vinifera brassinosteroid-6-oxidase gene were 2,and the up-expression fold of pathogenesis-related protein 1 gene were 5. For the expression of V.vinifera 4-coumarate:CoA ligase gene was a half of CK. At Campbell,the up-expression folds of V.vinifera brassinosteroid-6-oxidase gene,3-deoxy-D-arabino-heptulosonate 7-phosphate synthase gene and purple acid phosphatase 3-like gene in roots were 2,there was no obvious change for others,and those of pathogenesis-related protein 1 gene varied from 2 to 3. The experiment indicated that,under Mn stress,the tolerance of both the grape varieties to excess Mn stress responded through the different expressions of stress resistance gene,and phosphoric acid hydrolases also had a synergistic effect. There were the different regulatory approaches between roots and leaves of these genes due to the different ways of manganese enrichment in two grape varieties. From the different gene expressions,pathogenesis-related protein 1 gene and V.vinifera brassinosteroid-6-oxidase gene had the most significant manifestations,and they may be at an important position in the way of resistance to excess manganese.

Key words: Vitis vinifera, manganese, gene expression, qRT-PCR