AM真菌对红花生长及其有效成分的影响

doi:10.13866/j.azr.2019.04.18

Abstract

Abstract: Arbuscular mycorrhizas (AM) fungi is widely distributed excellent microbial resources in soil ecosystems and it is often used in medicinal plants with important economic value.In this study, Carthamus tinctorius was inoculated with different AM fungi: Glomus mosseae, Glomus intraradices and Mixed AM fungi (G.mosseae, G.etunicatum, G.microagregatum, G.caledonium, G.cladoideum and G.intraradices), respectively and inoculated with the same amount of inactivated bacteria as the control group.The inoculation effect of AM fungi was identified by measuring the infection rate and spore density, and the content of HSYA and kaempferol in C.tinctorius velvet was determined by high performance liquid chromatography at different picking stages.The purposes of this study were to investigate the effects of AM fungi on the growth and active constituents of C.tinctorius, and to analyze the effects of different flowering stages on HSYA and kaempferol so as to provide an important theoretical basis for promoting agricultural production of C.tinctorius in Xinjiang.We measured the content of hydroxysafflor yellow A and Kaempferol in C.tinctorius velvet by the high performance liquid chromatography method at different picking stages.The results showed that AM fungi could significantly promote the accumulation of aboveground parts, underground roots and floral biomass of C.tinctorius.In terms of the synthesis of secondary metabolites, there was no significant difference in the content of hydroxysafflor yellow A and Kaempferol in C.tinctorius under different AM fungi treatments and different growth stages.

Key words: arbuscular mycorrhizas fungi, mycorrhizal infection rate, soil spore density, secondary metabolite

ZHAO Xiang, ZENG Guang-ping, YANG Pan, ZHAI Ya-ping, ZHANG Xia, LIU Hong-ling. Effects of Arbuscular Mycorrhizal Fungi on Growth and Active Constituents of Carthamus tinctorius[J].Arid Zone Research, 2019, 36(4): 935-942.

References

[1] 梁慧珍,董薇,余永亮,等.国内外红花种质资源研究进展[J].安徽农业科学,2015,43(16):71-74.
[Liang Huizhen,Dong Wei,Yu Yongliang,et al.Advances in studies on safflower (Carthamus tinctorius L.) at home and abroad [J].Journal of Anhui Agricultural Sciences,2015,43(16):71-74.]
[2] 孙嘉磊,覃瑞,邢永忠,等.基于测序技术的红花基因组学研究进展[J].分子植物育种,2018,16(18):5934-5939.
[Sun Jialei,Qin Rui,Xing Yongzhong,et al.Research progress of safflower genomics based on sequencing technology[J].Molecular Plant Breeding,2018,16(18):5934-5939.]
[3] Motamedi M,Karimmojeni H,Sini F G.Evaluation of allelopathic potential of safflower genotype(Carthamus tinctorius L.)[J].Journal of Plant Protection Research,2016,56(4):364-367.
[4] 扈晓佳,殷莎,袁婷婷,等.红花的化学成分及其药理活性研究进展[J].药学实践杂志,2013,31(3):161-168.
[Hu Xiaojia,Yin Sha,Yuan Tingting,et al.Chemical constituents and pharmacological activities of Carthamus tinctorius L.[J].Journal of Pharmaceutical Practice,2013,31(3):161-168.]
[5] 沈冰冰,张松,朱启仁,等.羟基红花黄色素A减轻大鼠心肌细胞缺氧/复氧损伤[J].基础医学与临床,2018,28(4):480-484.
[Shen Bingbing,Zhang Song,Zhu Qiren,et al.Hydroxysafflor yellow A reduces anoxia/reoxygenation-induced injury in rat cardiomyocytes[J].Basic & Clinical Medicine,2018,28(4):480-484.]
[6] Kim S H,Hwang K A,Choi K C.Treatment with kaempferol suppresses breast cancer cell growth caused by estrogenand triclosan in cellular and xenograft breast cancer models[J].The Journal of Nutritional Biochemistry,2016,28:70-82.
[7] 王英男,陶爽,华晓雨,等.盐碱胁迫下AM真菌对羊草生长及生理代谢的影响[J].生态学报,2018,38(6):2187-2194.
[Wang Yingnan,Tao Shuang,Hua Xiaoyu,et al.Effects of arbuscular mycorrhizal fungi on the growth and physiological metabolism of Leymus chinensis under salt-alkali stress[J].Acta Ecologica Sinica,2018,38(6):2187-2194.]
[8] 刘兆娜,郭绍霞,李伟.AM真菌对百合生长和生理特性的影响[J].草业学报,2017,26(11):85-93.
[Liu Zhaona,Guo Shaoxia,Li Wei.Effect of arbuscular mycorrhizal fungi on growth and physiological characteristics of Lilium brownii[J].Acta Prataculturae Sinica,2017,26(11):85-93.]
[9] 吴楠,张静,王玥,等.积雪和丛枝菌根真菌网络对尖喙牻牛儿苗幼苗生长的影响[J].干旱区研究,2018,35(3):624-632.
[Wu Nan,Zhang Jing,Wang Yue,et al.Effects of snow cover and arbuscular mycorrhizal fungi network on the seedling growth of Erodium oxyrrhynchum[J].Arid Zone Research,2018,35(3):624-632.]
[10] Estrada B,Aroca R,Maathuis F J M,et al.Arbuscular mycorrhizal fungi native from a Mediterranean saline area enhance maize tolerance to salinity through improved ion homeostasis[J].Plant,Cell and Environment,2013,36(10):1771-1782.
[11] 叶佳舒,李涛,胡亚军,等.干旱条件下AM真菌对植物生长和土壤水稳定性团聚体的影响[J].生态学报,2013,33(4):1080-1088.
[Ye Jiashu,Li Tao,Hu Yajun,et al.Influences of AM fungi on plant growth and water-stable soil aggregates under drought stresses[J].Acta Ecologica Sinica,2013,33(4):1080-1088.]
[12] 曾广萍,张霞,刘红玲,等.盐胁迫下AM真菌对红花耐盐性的影响[J].植物生理学报,2011,47(11):1069-1074.
[Zeng Guangping,Zhang Xia,Liu Hongling,et al.Effect of AM fungi on salt tolerance of Carthamus tinctorius L.under salt stress[J].Plant Physiology Journal,2011,47(11):1069-1074.]
[13] Hoagland D R,Arnon D I.The water culture method for growing plants without soil[J].California Agricultural Experimerit Station Bulletin,1938,347(1):1-39.
[14] 刘润进,陈应龙.菌根学[M].北京: 科学出版社,2007.
[Liu Ruijin,Chen Yinglong.Mycorrhizology[M].Beijing: Science Press,2007.]
[15] 峥嵘,白淑兰,李龙,等.不同季节土庄绣线菊根围丛枝菌根真菌群落差异性研究[J].干旱区研究,2017,34(5):1049-1055.
[Zheng Rong,Bai Shulan,Li Long,et al.Seasonal variation of arbuscular mycorrhizal fungal communities in rhizosphere of Spiraea pubescens[J].Arid Zone Research,2017,34(5):1049-1055.]
[16] 丁丽丽,段陈平,李芳,等.红花不同采收期及不同部位中羟基红花黄色素A及山奈素的含量变化[J].沈阳药科大学学报,2015,32(1):65-69.
[Ding Lili,Duan Chenping,Li Fang,et al.The determination of hydroxysafflor yellow A and kaempferol in Carthamus tinctorrius L.by different collecting time and parts[J].Journal of Shenyang Pharmaceutical University,2015,32(1):65-69.]
[17] 苏英英,卢新义,孙宝平,等.中国药典2010年版红花质量标准中山柰素含量测定方法的探讨[J].中国医院药学杂志,2015,35(15):1427-1430.
[Su Yingying,Lu Xinyi,Sun Baoping,et al.Investigation on analytical method of kaempferol in Carthamus tinctorius L.in Chinese Pharmacopoeia (2010 Edition)[J].Chinese Journal of Hospital Pharmacy,2015,35(15):1427-1430.]
[18] 郭巧生,程俐陶,刘作易.丛枝菌根真菌对半夏产量及化学成分的影响[J].中国中药杂志,2010,35(3):333-338.
[Guo Qiaosheng,Cheng Litao,Liu Zuoyi.Study on influence of arbuscular mycorrhizal fungi Pinellia ternata yield and chemical composition[J].China Journal of Chinese Materia Medica,2010,35(3):333-338.]
[19] 郭兰萍,汪洪钢,黄璐琦,等.泡囊丛枝菌根(AM)对苍术生长发育及挥发油成分的影响[J].中国中药杂志,2006,31(18):1491-1495.
[Guo Lanping,Wang Honggang,Huang Luqi,et al.Effects of arbuscular mycorrhizae on growth and essential oil of Atractylodes lancea[J].China Journal of Chinese Materia Medica,2006,31(18):1491-1495.]
[20] Yang T,Dai C C.Interactions of two endophytic fungi colonizing Atractylodes lancea and effects on the host’s essential oils[J].Acta Ecologica Sinica,2013,33(2):87-93.
[21] 刘珮,马慧,智颖飙,等.9种典型荒漠植物生态化学计量学特征分析[J].干旱区研究,2018,35(1):207-216.
[Liu Pei,Ma Hui,Zhi Yingbiao,et al.Ecological stoichiometric differences of nine typical eremophyte species[J].Arid Zone Research,2018,35(1):207-216.]
[22] 阎秀峰,王洋,李一蒙.植物次生代谢及其与环境的关系[J].生态学报,2007,26(6):2554-2560.
[Yan Xiufeng,Wang Yang,Li Yimeng.Plant secondary metabolism and its response to environment[J].Acta Ecologica Sinica,2007,26(6):2554-2560.]
[23] Yadav K, Aggarwal A, Singh N.Arbuscular mycorrhizal fungi (AMF) induced acclimatization,growth enhancement and colchicine content of micropropagated Gloriosa superba L.plantlets[J].Industrial Crops and Products,2013,45:88-93.
[24] 杨敏,张杰,张德全,等.丛枝菌根真菌对掌叶大黄产量及次生代谢产物的影响[J].中国试验方剂学杂志,2018,24(10):51-55.
[Yang Min,Zhang Jie,Zhang Dequan,et al.Effect of arbuscular mycorrhizal on yield and secondary metabolites of Rheum palmatum[J].Chinese Journal of Experimental Traditional Medical Formulae,2018,24(10):51-55.]
[25] Vierheilig H,Lerat S,Piché Y.Systemic inhibition of arbuscular mycorrhiza development by root exudates of cucumber plants colonized by Glomus mosseae[J].Mycorrhiza,2003,13(3):167-170.
[26] Vierheilig H,Garcia-Garrido J M,Wyss U.Systemic suppression of mycorrhizal colonization of barley roots already colonized by AM fungi[J].Soil Biology and Biochemistry,2000,32(5):589-595.
[27] 赵昕,王博文,阎秀峰.丛枝菌根对喜树幼苗喜树碱含量的影响[J].生态学报,2006,26(4):1057-1062.
[Zhao Xin,Wang Bowen,Yan Xiufeng.Effect of arbuscular mycorrhiza on camptothecin content in Camptotheca acuminata seedlings[J].Acta Ecologica Sinica,2006,26(4):1057-1062.]
[28] 于洋,于涛,王洋,等.接种时期对丛枝菌根喜树幼苗喜树碱含量的影响[J].植物生态学报,2010,3(6):687-694.
[Yu Yang,Yu Tao,Wang Yang,et al.Effect of inoculation time on camptothecin content in arbuscular mycorrhizal Camptotheca acuminate seedlings[J].Chinese Journal of Plant Ecology,2010,3(6):687-694.]
[29] Zubek S,Mielcarek S,Turnau K.Hypericin and pseudohypericin concentrations of a valuable medicinal plant Hypericum perforatum L.are enhanced by arbuscular mycorrhizal fungi[J].Mycorrhiza,2011,22(2):149-152.
[30] 赵则海,于景华,杨逢建,等.人为扰动对乌拉尔甘草不同部位甘草酸与总黄酮含量的影响[J].生态学报,2004,24(12):2800-2803.
[Zhao Zehai,Yu Jinghua,Yang Fengjian,et al.Influences of artificial disturbance degrees on the contents of Glycyrrhizic acid and flavonoids in different parts of Glycyrrhiza uralensis[J].Acta Ecologica Sinica,2004,24(12):2800-2803.]
[31] 刘金荣,赵文斌,王航宇,等.不同生长期栽培甘草的产量及有效成分分析比较[J].上海中医药杂志,2004,38(11):56-58.
[Liu Jinrong,Zhao Wenbin,Wang Hangyu,et al.Output of cultivated Glycyrrhizia in different growth stages and analytical comparison of its active ingredients[J].Shanghai Journal of Traditional Chinese Medicine,2004,38(11):56-58.]
[32] Yan X F,Wu S X,Wang Y,et al.Soil nutrient factors related to salidroside production of Rhodiola sachalinensis distributed in Chang Bai Mountain[J].Environmental and Experimental Botany,2004,52(3):267-276.
[33] 刘燕,徐志才,唐立松.叶圈微生物的群落结构与生态功能研究进展[J].干旱区研究,2018,35(2):340-345.
[Liu Yan,Xu Zhicai,Tang Lisong.Research progress of community structure and ecological dunctions of phyllosphere microorganisms[J].Arid Zone Research,2018,35(2):340-345.]
[34] Liu H L,Tan Y,Nell M,et al.Arbuscular mycorrhizal fungal colonization of Glycyrrhiza glabra roots enhances plant biomass,phosphorus uptake and concentration of root secondary metabolites[J].Journal of Arid Land,2014,6(2):186-194.
[35] 贺学礼,杨欢,杨莹莹,等.沙棘AM真菌孢子形态结构及其生态适应性[J].干旱区研究,2013,30(1):96-100.
[He Xueli,Yang Huan,Yang Yingying,et al.Morphological structure and ecological adaptability of arbuscular mycorrhizal fungi in the rhizosphere of Hippophae rhamnoides[J].Arid Zone Research,2013,30(1):96-100.]

Effects of Arbuscular Mycorrhizal Fungi on Growth and Active Constituents of Carthamus tinctorius

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