干旱区研究

干旱区研究 ›› 2012, Vol. 29 ›› Issue (6): 1039-1045.

• 植物与植物生理 • 上一篇    下一篇

盐胁迫条件下骆驼刺与绿豆光合日变化特征及午休现象的成因

吾木提汗•卡克木1，海利力•库尔班2，陈其军3，王学臣3   

  1. 1. 新疆农业大学农学院，新疆 乌鲁木齐 830052；2．新疆农业大学林学与园艺学院，新疆 乌鲁木齐 830052； 3. 中国农业大学生命科学学院，北京 100193
  • 收稿日期:2011-11-28 修回日期:2012-01-12 出版日期:2012-11-15 发布日期:2012-11-19
  • 通讯作者: 海利力?库尔班. E-mail: hkurban@163.com
  • 作者简介:吾木提汗?卡克木(1983-)，女，新疆昌吉人，硕士研究生，主要从事植物逆境生理学研究. E-mail: wmet@163.com
  • 基金资助:

    国家自然科学基金项目（30760020）

Study on Daily Change and Midday Depression of Photosynthesis of Alhagi pseudoalhagi and Vigna radiata under Salt Stress

UMETHAN Kakem 1, HALIL Kurban 2, CHEN Qi-jun 3, WANG Xue-chen3   

  1. 1. College of Agriculture, Xinjiang Agricultural University, Urumqi 830052, Xinjiang, China;2. College of Forestry and Horticulture, Xinjiang Agricultural University, Urumqi 830052, Xinjiang, China;3. College of Life Sciences, China Agricultural University, Beijing 100193, China
  • Received:2011-11-28 Revised:2012-01-12 Online:2012-11-15 Published:2012-11-19

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摘要： 为了研究盐生豆科植物骆驼刺与盐敏感植物绿豆在盐胁迫下光合日变化与光合“午休”现象的成因，在网室培养，并用0 mmol•L-1(对照)、50 mmol•L-1、100mmol•L-1、200 mmol•L-1 NaCl处理2周，测定光合作用日变化、叶绿素含量和Rubisco大亚基基因的表达等参数。结果表明：未经盐处理时，骆驼刺和绿豆光合日变化均呈单峰型；经盐处理后，两者光合日变化响应有明显差异。骆驼刺50～200 mmol•L-1NaCl处理后，其净光合速率（Pn）均比未处理(对照)上升速率快，而其他处理峰值与对照之间没有显著差异；绿豆对照光合速率的上升则比盐处理的快，盐处理光合速率峰值低于对照。骆驼刺50 mmol•L-1、100 mmol•L-1和绿豆50 mmol•L-1处理Pn没有出现“午休”现象，而骆驼刺200 mmol•L-1处理与绿豆100 mmol•L-1处理出现“午休”现象。骆驼刺在重度盐胁迫下，由于气孔导度（Gs）下降而引起轻微的“午休”现象，而盐处理绿豆中Rubisco被抑制，对Pn 午休的贡献比其Gs的贡献更为显著。在绿豆中大亚基基因（rbcL）的表达量随着盐胁迫的提高而下降。骆驼刺中rbcL的表达在50 mmol•L-1和100 mmol•L-1处理下有所增加，而在200 mmol•L-1处理显著降低。绿豆和骆驼刺的总叶绿素含量及其Chl a/b比值的动态变化表明，绿豆PSI复合体下降速率较PSII快；与此相反，骆驼刺盐处理后PSI复合体浓度的增加却比PSII复合体快。

关键词: 骆驼刺[（Alhagi pseudoalhagi）, 绿豆（Vigna radiata）, 盐胁迫, 光合日变化, 光合“午休”, 叶绿素

Abstract: This experiment was designed to investigate the factors affecting the daily change of photosynthesis in the salttolerant leguminous plant Alhagi pseudoalhagi by comparing with saltsensitive Vigna radiata. The plants were cultivated in a greenhouse and subjected to 0 (controlled), 50, 100 and 200 mmol•L-1 NaCl treatments for 2  weeks.The daily change of photosynthesis, chlorophyll content and expression of Rubisco large subunit gene were measured. The results showed that the curves of controlled plants of both A. pseudoalhagi and V. radiata were unimodal, while the response of photosynthetic daily change with increasing salinity was different between the saltsensitive plant V. radiata and the salttolerant one A. pseudoalhagi. For A. pseudoalhagi, the rates of Pn in 50-200 mmol•L-1 NaCltreated plants increased faster than that in the untreated control plants, and there was no significant difference among other treatments in the max peak of Pn values. The increase of photosynthetic rate of V. radiata was faster in the controlled plants than that in salinity treatments, and salttreated plants had a lower photosynthetic rate than the untreated plants. There was no midday depression for A. pseudoalhagi in 50-100 mmol•L-1 and V. radiata[ in 50 mmol•L-1 NaCl, which was observed in A. pseudoalhagi 200 mmol•L-1 treatment and in V. radiata 100 mmol•L-1 treatment. In A. pseudoalhagi, the ‘midday depression’ was mainly caused by decreased Gs under severe salt stress, while in V. radiata, the contribution of the inhibition of Rubisco to the Pn‘midday depression’ was more significant than that of the Gs. In V. radiata, the expression of rbcL was decreased with increasing salt stress, and in A. pseudoalhagi, the expression of rbcL was increased at 50 and 100 mmol•L-1, however, it was decreased at 200 mmol•L-1 NaCl.

Key words: Alhagi pseudoalhagi, Vigna radiata, salt stress, photosynthetic daily change, photosynthetic midday depression, chlorophyll