干旱区研究

干旱区研究 ›› 2011, Vol. 28 ›› Issue (6): 1064-1070.

• 动物与微生物 • 上一篇    

转铜绿假单胞杆菌KatB基因烟草获得及其抗逆性鉴定

 喻娜, 程晨, 孙辉, 徐登献, 祝建波   

  1. 石河子大学生命科学学院 农业生物技术重点实验室，新疆 石河子 832003
  • 收稿日期:2010-11-20 修回日期:2010-12-22 出版日期:2011-11-15 发布日期:2012-01-16
  • 作者简介:喻娜（1986-），女，在读硕士研究生，主要从事植物基因工程研究. E-mail: yuna421@163.com
  • 基金资助:

    国家转基因专项(2008ZX080052004)；兵团博士基金(2006jc07)

Gain of the KatB Genetic Tobacco and Analysis on Stress Resistance of Transgenic Tobacco

 YU  Na, CHENG  Chen, SUN  Hui, XU  Deng-Xian, ZHU  Jian-Bo   

  1. Laboratory of Agricultural Biotechnology, College of Life Science, Shihezi University, Shihezi 832003, Xinjiang, China
  • Received:2010-11-20 Revised:2010-12-22 Published:2011-11-15 Online:2012-01-16

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摘要： 以NC 89烟草（Nicotiana tabacum）为材料，通过农杆菌介导法获得转铜绿假单胞杆菌（Pseudomonas aeruginosa）KatB基因植株，利用PCR和RT-PCR对转化烟草进行鉴定，最后通过水分胁迫、盐胁迫、过氧化氢胁迫进行抗逆性分析。结果表明：与野生型植株相比，在缺水条件下的第13 d，300 mmol/L的NaCl胁迫条件下第20 d和0.3mmol/L的H2O2胁迫条件下第33 d，转KatB基因烟草的叶片相对含水量、伤害率、过氧化氢酶（CAT）、丙二醛（MAD）、光系统Ⅱ（PSII）相对量子产率各种生理指标都显著优于野生型（WT）烟草，转KatB基因烟草表现出了较强的抗逆性，说明KatB基因在抗逆基因工程方面具有较好的应用前景。

关键词: 铜绿假单胞杆菌, KatB基因, 烟草, 抗逆性

Abstract: NC 89 tobacco plants were transformed via Agrobacterium tumefaciens. PCR and RT-CR indicate that the foreign gene KatB of Pseudomonas aeruginosa has been integrated into the genome of tobacco. Stress resistance tests were carried out under water stress, 300 mmol/L NaCl stress and 0.3 mmol/L H2O2 stress. The results proved that the physiological indexes including leaf relative water content, MDA activity, relative conductivity and maximum photochemical yield of PSII of KatB tobacco were evidently higher than those of wild tobacco on the 13th day of water stress, 20th day of 300 mmol/L NaCl stress and the 33rd day of 0.3 mmol/L H2O2 stress. The stress resistance of transgenic plants was high, which testified that there is a good application prospect of KatB gene in stress resistance of genetic engineering.

Key words: Pseudomonas aeruginosa, KatB gene, tobacco, stress resistance