Abstract: Alhagi sparsifolia Shap.is a perennial subshrub in the Leguminosae family.It grows mainly in Central Asia and Xinjiang Uygur Autonomous Region,China,and has been used in traditional Uygur medicine for a long time.It is widely used for treating physically weak,dizzy,cold,headache,intestinal constipation and arthrodynia.It mainly contains flavonoids,sterols, alkaloids, fatty acids and polysaccharides.There is no any report on the fingerprint chromatogram or chemical marker of A.sparsifolia yet.In order to develop the fingerprint chromatogram and provide the basis for the effective control of A.sparsifilia quality, the samples were separated by RPHPLC on a Waters Atlantis T3 C18 (4.6 mm×250 mm, 5 μm), with acetonitrile and 0.2% formic acid as mobile phase in a linear gradient manner.The flow rate was 1.0 mL·min^-1, the wavelength of detection was 280 nm, and the acquisition time was 100 min.HPLC fingerprint chromatograms of 10 batches of A.sparsifolia were analyzed.The HPLC fingerprint chromatogram of A.sparsifolia was developed with satisfactory resolution and 11 common fingerprint peaks with high similarity of correlation coefficients above 0.9.The peak No.10 and peak No.11 were the main compounds of A. sparsifolia, with the retention time matching isorhamnetin→3→O→β→D→galactosyl→(6→1) →α→L→rhamnoside and isorhamnetin→3→O→β→Drutinoside by HPLC, respectively. The results met the requirements of fingerprint technology. It was identified that the fingerprint method was stable and reliable, and could be used for the quality control of medicinal A. sparsifolia.

Key words: Alhagi sparsifolia Shap.')">Alhagi sparsifolia Shap., HPLC, fingerprint chromatogram