干旱区研究

干旱区研究 >

2025 , Vol. 42 >Issue 2: 312 - 320

DOI: https://doi.org/10.13866/j.azr.2025.02.11

植物生态

干旱、盐分和酸胁迫对药用植物马利筋和苋菜种子萌发的影响

  • 贾风勤 ,
  • 黄敏桃 ,
  • 邓利 ,
  • 李金玲 ,
  • 梁梅华 ,
  • 伊洪伟 ,
  • 宋希娟
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  • 广西生态工程职业技术学院林业工程学院，广西 柳州 545004
贾风勤（1973-），女，博士，教授，主要从事种子生态研究. E-mail: 1002249491@qq.com
宋希娟. E-mail: 119248283@qq.com

收稿日期: 2024-05-16

  修回日期: 2024-10-10

  网络出版日期: 2025-02-21

基金资助

广西生态工程职业技术学院人才引进科研专项(GXSTKYZX202307003);新疆维吾尔自治区天山青年计划项目(2018Q028)

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Effects of drought, salt, and acidity stresses on Asclepias curassavica and Amaranthus tricolor seed germination

  • JIA Fengqin ,
  • HUANG Mintao ,
  • DENG Li ,
  • LI Jinling ,
  • LIANG Meihua ,
  • YI Hongwei ,
  • SONG Xijuan
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  • College of Forestry Engineering, Guangxi Eco-Engineering Vocational and Technical College, Liuzhou 545004, Guangxi, China

Received date: 2024-05-16

  Revised date: 2024-10-10

  Online published: 2025-02-21

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摘要

以药用植物马利筋和苋菜为材料，采用聚乙二醇（PEG-6000）、NaCl和pH梯度溶液模拟干旱、盐分和酸胁迫环境，研究两种植物种子萌发对环境胁迫的响应程度，为探讨其在干旱、盐碱地区的引种栽培和高品质药材生产提供理论依据。结果表明：不同浓度PEG和NaCl显著抑制马利筋和苋菜的种子萌发，总体表现为萌发率、发芽指数均随浓度的增加而降低；将经过14 d胁迫后的未萌发种子复水处理，出现快速补偿萌发；复水处理后仍未萌发种子TTC染色结果显示种子依旧保持活力，两种药用植物具活力种子占比均值分别为84%和90%，显著高于对照或未显著降低；pH值为3~6范围内，马利筋和苋菜种子萌发率均超过57%和83%，未萌发种子复水萌发和TTC染色结果表明胁迫处理造成约10%的马利筋种子和15%的苋菜种子死亡，两种药用植物具活力种子占比均值分别高达91%和87%。马利筋和苋菜种子在萌发过程中对干旱和盐胁迫具有一定的耐性，对酸胁迫具有较强的耐性，两种植物可采取提前、延迟或休眠的萌发策略以适应不同胁迫环境，本研究厘清了马利筋和苋菜种子萌发在3种非生物胁迫因子处理下的萌发特征及差异，为两种药用植物生产和品质提高提供了理论依据。

关键词： 药用植物; 马利筋; 苋菜; 种子萌发; 耐受性

本文引用格式

贾风勤 , 黄敏桃 , 邓利 , 李金玲 , 梁梅华 , 伊洪伟 , 宋希娟 . 干旱、盐分和酸胁迫对药用植物马利筋和苋菜种子萌发的影响[J]. 干旱区研究, 2025 , 42(2) : 312 -320 . DOI: 10.13866/j.azr.2025.02.11

Abstract

The effect of environmental factors on the seed germination of two medicinal plants, Asclepias curassavica and Amaranthus tricolor, was studied using PEG-6000, NaCl, and pH gradient solutions to simulate drought, salt, and acidity stresses, respectively. The results provide the basis for their cultivation in arid, saline-alkali areas and the production of high-quality medicinal materials. The findings revealed that the seed germination percentage and index of A. curassavica and A. tricolor decreased with increasing PEG and NaCl concentrations. After 14 days of stress, the nongerminated seeds could rapidly germinate after rehydration and did not lose their vitality. TTC staining of the nongerminated seeds after rehydration revealed that the seeds remained active; the viable seed proportion of the two medicinal plants was significantly higher than in the control or did not decrease significantly, with mean values of 84% and 90%, respectively. At pH 3-6, the A. curassavica and A. tricolor seed germination percentages were 57% and 83%, while the nongerminated seeds of A. curassavica and A. tricolor lost their vitality. The percentages of dead seeds were 10% and 15%, and the proportions of viable seeds were 91% and 87%, respectively, for A. curassavica and A. tricolor. The two medicinal plants displayed a certain tolerance to drought and salt stress and a strong tolerance to acid stress during germination. The two plants can adopt different germination strategies, such as advanced or delayed germination or dormancy, to adapt to their environmental stresses. This study clarified the seed germination characteristics and differences of A. curassavica and A. tricolor under three abiotic stress factors. It provides a theoretical basis for producing and improving the quality of two medical plants.

Key words： medicinal plant; Asclepias curassavica; Amaranthus tricolor; seed germination; tolerance

参考文献

[1] Flowers T J, Yeo A R. Ion relations of plants under drought and salinity[J]. Australian Journal of Plant Physiology, 1986, 13(1): 75-91.
[2] Acosta-Motos J R, Ortu?o M F, Bernal-Vicente A, et al. Plant responses to salt stress: Adaptive mechanisms[J]. Agronomy, 2017, 7(1): 18-56.
[3] Munns R. Comparative physiology of salt and water stress[J]. Plant, Cell and Environment, 2002, 25: 239-250.
[4] Cristancbo R J A, Hanafi M N, Syed Omar S R, et al. Aluminum speciation of amended acid tropical soil and its effects on plant root growth[J]. Journal of Plant Nutrition, 2014, 37: 811-827.
[5] Anjum S A, Xie X Y, Wang L C, et al. Morphological,physiological and biochemical responses of plants to drought stress[J]. African Journal of Agricultural Research, 2011, 6(9): 2026-2032.
[6] Ludlow M M, Muchow R C. A critical evaluation of traits for improving crop yields in water-limited environments[J]. Advances in Agronomy, 1990, 43: 107-153.
[7] 戴炜, 唐宇翀, 喻娜, 等. 马利筋引种繁殖技术研究[J]. 园艺与种苗, 2021, 41(4): 52-53, 64.
  [Dai Wei, Tang Yuchong, Yu Na, et al. Study on introduction an proagation technology of Asclepias curassavica[J]. Horticulture and Seed, 2021, 41(4): 52-53, 64. ]
[8] 陆云梅, 黄仁华, 刘鸿宇. 硒和丛枝菌根真菌处理对苋菜生长及硒积累的影响[J]. 福建农业学报, 2024, 39(5): 563-570.
  [Lu Yunmei, Huang Renhua, Liu Hongyu. Effects of selenium and arbuscular mycorrhizal fungi applications on growth, photosynthesis, and selenium accumulation of Amaranthus tricolor L.[J]. Fujian Journal of Agricultural Sciences, 2024, 39(5): 563-570. ]
[9] 袁玮琪. 马利筋新型C21甾体化合物Asclepiasterol逆转P-gp介导肿瘤多药耐药的作用及机制研究[D]. 广州: 暨南大学, 2016.
  [Yuan Weiqi. The Effect and Mechanism Study of Asclepiasterol, A Novel C21 Steroidal Glycoside Derived from Asclepias curassavica Reversing P-gp-mediated Multidrug Resistance[D]. Guangzhou: Jinan University, 2016. ]
[10] 王世敏, 程金鹏. 马利筋组织培养初探[J]. 安徽农业科学, 2011, 39(25): 15263-15274, 15267.
  [Wang Shimin, Cheng Jinpeng. Preliminary research on the tissue culture of Asclepsus curassavica[J]. Journal of Anhui Agricultural Sciences, 2011, 39(25): 15263-15274, 15267. ]
[11] 王廷芹, 甘秋霞, 李倩如. 盐胁迫对苋菜种子的发芽及幼苗生长的影响[J]. 贵州大学学报(自然科学版), 2021, 38(1): 10-15, 32.
  [Wang Yanqin, Gan Qiuxia, Li Qianru. Effect of salt stress on seed germination and seedling growth of Amaranth[J]. Journal of Guizhou University (Natural Sciences), 2021, 38(1): 10-15, 32. ]
[12] 蒋妮, 覃柳燕, 李力, 等. 环境胁迫对药用植物次生代谢产物的影响[J]. 湖北农业科学, 2012, 51(8): 1528-1532.
  [Jiang Ni, Qin Liuyan, Li Li, et al. Influence of environmental stress on medicinal plant secondary metabolites[J]. Hubei Agriultural Sciences, 2012, 51(8): 1528-1532. ]
[13] Michel B E, Kaufmann M R. The osmotic potential of polyethylene glycol 6000[J]. Plant Physiology, 1973, 51(5): 914-916.
[14] 张春平, 何平, 何俊星, 等. 药用保护植物黄连种子萌发特性研究[J]. 西南大学学报(自然科学版), 2008, 30(9): 89-93.
  [Zhang Chunping, He Ping, He Junxing, et al. Characteristics of seed germination of the protected medicinal plant Coptis chinensis[J]. Journal of Southwest University (Natural Science Edition), 2008, 30(9): 89-93. ]
[15] 于婵, 张依琳, 李秋莹, 等. 盐碱胁迫对牛至种子萌发和幼苗生理生化特性的影响[J]. 草地学报, 2024, 32(6): 1882-1892.
  [Yu Chan, Zhang Yilin, Li Qiuying, et al. Effects of saline-alkali stresses on seed germination and seedling physiological and biochemical characteristics of Oregano vulgare[J]. Acta Agrestia Sinica, 2024, 32(6): 1882-1892. ]
[16] Samarah N, Alqudah A. Effects of late-terminal drought stress on seed germination and vigor of barley (Hordeum vulgare L.)[J]. Archives of Agromomy and Soil Science, 2011, 57(1): 27-32.
[17] 徐宁伟, 路斌, 高慧, 等. 盐胁迫对两种苋科植物种子萌发的影响[J]. 干旱区资源与环境, 2021, 35(8): 138-143.
  [Xu Ningwei, Lu Bin, Gao Hui, et al. Effects of salt stress on seed germination of two Amaranthaceae species[J]. Journal of Arid Land Resources and Environment, 2021, 35(8): 138-143. ]
[18] 高志昊, 李雪颖, 兰剑, 等. 干旱胁迫条件下不同饲用燕麦品种种子萌发指标比较与评价[J]. 草地学报, 2022, 30(5): 1210-1218.
  [Gao Zhihao, Li Xueying, Lan Jian, et al. Comparison and evaluation of seed germination indexes of different forage-type Oat cultivars under PEG-6000 stress[J]. Acta Agrestia Sinica, 2022, 30(5): 1210-1218. ]
[19] Lai L M, Chen L J, Zheng M Q, et al. Seed germination and seedling growth of five desert plants and their relevance to vegetation restoration[J]. Ecology and Evolution, 2019, 9(4): 2160-2170.
[20] 李畅, 苏家乐, 刘晓青, 等. 干旱胁迫对鹿角杜鹃种子萌发和幼苗生理特性的影响[J]. 西北植物学报, 2015, 35(7): 1421-1427.
  [Li Chang, Su Jiale, Liu Xiaoqing, et al. Effects of drought stress on seed germination and seedling physiological characteristics of Rhododendron latoucheae[J]. Acta Botanica Boreali-Occidentalia Sinica, 2015, 35(7): 1421-1427. ]
[21] 王亚, 刘延, 范志伟, 等. 入侵植物石茅种子萌发及幼苗生长对干旱胁迫的响应特征[J]. 草业科学, 2023, 40(8): 2020-2027.
  [Wang Ya, Liu Yan, Fan Zhiwei, et al. Response characteristics of seed germination and seedling growth of the invasive plant Sorghum halepense[J]. Pratacultural Science, 2023, 40(8): 2020-2027. ]
[22] Li R, Shi F, Fukuda K. Interactive effects of salt and alkali stresses on seed germination, germination recovery, and seedling growth of a halophyte Spartina alterniflora (Poaceae)[J]. South African Journal of Botany, 2010, 76: 380-387.
[23] 李天永, 严子柱, 姜生秀. 两种独行菜种子萌发对不同浓度NaCl胁迫的响应[J]. 草地学报, 2021, 29(1): 88-94.
  [Li Tianyong, Yan Zizhu, Jiang Shengxiu. Responses of seed germination of two Lepidium species to different concentrations of NaCl stress[J]. Acta Agrestia Sinica, 2021, 29(1): 88-94. ]
[24] 曾荔琦, 曾嘉欣, 杨蕾蕾, 等. 罗汉果伯林2号种子形态结构及萌发特性[J]. 亚热带植物科学, 2023, 52(4):293-300.
  [Zeng Liqi, Zeng Jiaxin, Yang Leilei, et al. Seed morphology and germination characteristics of Siraitia grosvenorii Bolin No. 2[J]. Subtropical Plant Science, 2023, 52(4): 293-300. ]
[25] 何影, 马淼. 入侵植物意大利苍耳种子萌发对环境因子的响应[J]. 生态学报, 2018, 38(4): 1226-1234.
  [He Ying, Ma Miao. Responses of seed germination of the invasive plant Xanthium italicum to environmental factors[J]. Acta Ecologica Sinica, 2018, 38(4): 1226-1234. ]
[26] Chauhan B S, Gill G, Preston C. Factors affecting seed germination of little mallow (Malva parvilfora) in southern Australia[J]. Weed Science, 2006, 54(6): 1045-1050.
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