干旱区研究

干旱区研究 >

2023 , Vol. 40 >Issue 6: 905 - 915

DOI: https://doi.org/10.13866/j.azr.2023.06.06

水土资源

呼伦贝尔沙地樟子松人工林土壤细菌网络特征

  • 张颂安 ,
  • 刘轩 ,
  • 赵珮杉 ,
  • 高广磊 ,
  • 张英 ,
  • 丁国栋 ,
  • 柳叶 ,
  • 任悦
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  • 1.北京林业大学水土保持学院，林业生态工程教育部工程研究中心，北京 100083
    2.宁夏盐池毛乌素沙地生态系统国家定位观测研究站，宁夏 盐池 751500
    3.水土保持国家林业和草原局重点实验室，北京 100083
张颂安（1997-），女，硕士研究生，主要研究方向为荒漠化防治. E-mail: zhangsongan@bjfu.edu.cn

收稿日期: 2022-09-21

  修回日期: 2022-12-07

  网络出版日期: 2023-06-21

基金资助

内蒙古自治区科技计划项目(2022YFHH0131);中央高校基本科研业务费项目(2021ZY47)

收起

Soil bacterial networks in Pinus sylvestris var. mongolica plantations of the Hulunbuir Desert

  • Songan ZHANG ,
  • Xuan LIU ,
  • Peishan ZHAO ,
  • Guanglei GAO ,
  • Ying ZHANG ,
  • Guodong DING ,
  • Ye LIU ,
  • Yue REN
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  • 1. School of Soil and Water Conservation, Beijing Forestry University, Engineering Research Center of Forestry Ecological Engineering, Ministry of Education, Beijing 100083, China
    2. Yanchi Ecology Research Station of the Mu Us Desert, Yanchi 751500, Ningxia, China
    3. Key Laboratory of State Forestry and Grassland Administration on Soil and Water Conservation, Beijing 100083, China

Received date: 2022-09-21

  Revised date: 2022-12-07

  Online published: 2023-06-21

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摘要

为揭示呼伦贝尔沙地樟子松人工林土壤细菌相互关系，以呼伦贝尔沙地不同林龄樟子松人工林（25 a、34 a和43 a）为研究对象，以沙质草地为对照，采用分子生态网络分析法对不同土层（0~10 cm和10~20 cm）土壤细菌群落进行比较分析。结果表明：（1） 从25 a到43 a，土壤细菌网络总边数增多，平均路径长度降低。土壤深度由0~10 cm到10~20 cm，人工林土壤细菌网络总边数减少，平均路径长度升高。与沙质草地相比，人工林土壤细菌网络总边数较少。（2） 25 a人工林关键菌种隶属于嗜酸菌目（Acidimicrobiales）、RB41和MB-A2-108，34 a人工林关键菌种隶属于Gaiellales，43 a人工林关键菌种隶属于Gaiellales、RB41、Subgroup_7、Subgroup_6、和DA101_soil_group，草地关键菌种隶属于匿杆菌门（Latescibacteria）。（3） 全氮、氨氮、微生物碳含量和脲酶酶活性对土壤细菌网络中具有高中介中心性的部分细菌有显著正相关影响（P<0.05）；转化酶和过氧化氢酶活性、土壤含水量以及速效磷含量对土壤细菌网络中具有高中介中心性的部分细菌有显著负相关影响（P<0.05）；土壤有机质对土壤细菌网络中具有高中介中心性的部分细菌既有显著正相关影响又有显著负相关影响（P<0.05）。樟子松人工林从25 a到43 a，土壤细菌网络愈加复杂和紧密，土壤深度由0~10 cm到10~20 cm，网络复杂性和紧密度降低；与草地相比，人工林土壤细菌网络复杂性较低。43 a人工林土壤细菌网络关键菌种类型数量最多。另外，土壤细菌网络受土壤有机质影响最大。研究结果有助于深入理解呼伦贝尔沙地樟子松人工林土壤细菌群落，并为呼伦贝尔沙地樟子松人工林的可持续经营提供科技支撑。

关键词： 土壤微生物; 分子生态网络; 林龄; 土层; 樟子松; 呼伦贝尔沙地

本文引用格式

张颂安 , 刘轩 , 赵珮杉 , 高广磊 , 张英 , 丁国栋 , 柳叶 , 任悦 . 呼伦贝尔沙地樟子松人工林土壤细菌网络特征[J]. 干旱区研究, 2023 , 40(6) : 905 -915 . DOI: 10.13866/j.azr.2023.06.06

Abstract

To elucidate soil bacterial network interactions within Pinus sylvestris var. mongolica plantations in the Hulunbuir Desert. P. sylvestris plantations representing three different age groups (25 a, 34 a, and 43 a) and two soil layers (0-10 and 10-20 cm) were selected to assess their soil bacterial networks using molecular ecological network analysis and data from a referenced grassland. The numbers of network edges increased, the average path length reduced from 25 a to 43 a. While the number of network edges reduced and the average path length increased with soil layers from 0-10 cm to 10-20 cm. Compared with the grassland, the P. sylvestris plantations had a lower network edge, and the soil bacterial network was less complicated. The soil bacteria were found to belong to the Acidimicrobiales, RB41, and MB-A2-108 in the 25 a plantation, Gaiellales in the 34 a plantation, and Gaiellales, RB41, Subgroup_7, Subgroup_6, and DA101_soil_group in the 43 a plantation, Latescibacteria in the grassland. The soil bacterial network was significantly positively correlated with ammonia nitrogen, total nitrogen, microbial carbon content, and urease activities and significantly negatively correlated with invertase and catalase activities, soil water content and available phosphorus (P < 0.05). The soil organic matter had both positive and negative effects (P < 0.05). The soil bacterial network complexity and compactness increased from 25 a to 43 a. The opposite was found for the soil layers from 0-10 cm to 10-20 cm. Compared with the grassland, the soil bacterial network of the P. sylvestris plantation was less complicated. The keystone soil bacteria species were different among the three stand ages, and were greatest in stand 43 a. There were more keystone species in the plantation than the grassland. Soil physicochemical properties and enzymatic activity derived the soil bacterial network, and soil organic matter was the major influencing factor. This improved information contributed to a deep understanding of the soil bacterial community and provided a scientific and technological basis for the sustainable management of P. sylvestris plantations in the Hulunbuir Desert.

Key words： soil microbes; molecular ecological network; stand age; soil depth; Pinus sylvestris var. mongolica; the Hulunbuir Desert

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