不同供磷水平对紫花苜蓿根际微生物功能多样性的影响
收稿日期: 2022-03-10
修回日期: 2022-04-28
网络出版日期: 2022-10-25
基金资助
国家自然科学基金“制种苜蓿根瘤菌固氮效能对氮素输入的响应”(31760712)
Effect of different phosphorus supply levels on rhizosphere microbial functional diversity of Medicago sativa
Received date: 2022-03-10
Revised date: 2022-04-28
Online published: 2022-10-25
探讨不同供磷水平下紫花苜蓿根际微生物的代谢功能多样性,有助于进一步了解紫花苜蓿对肥料的高效利用机制。本研究设置5种不同供磷水平,通过田间试验,应用Biolog方法对紫花苜蓿根际微生物群落代谢特征进行研究。结果表明：（1） 施用磷肥可以显著提高紫花苜蓿根际微生物的代谢活性,供磷水平为P3（300 kg·hm-2）时,Simpson指数、Richness指数达到最大值,提高了微生物对不同分类碳源的利用。（2） 当磷肥浓度过高时,会显著降低微生物群落Simpson指数,同时也会降低微生物对各类碳源的利用率。（3） 主成分分析显示,不同供磷水平下根际微生物对不同碳源的利用率有显著差异,供磷水平为P4（450 kg·hm-2）时,微生物群落对各类碳源利用差异最小。
邢鏻木,李强,高原千惠,李宁 . 不同供磷水平对紫花苜蓿根际微生物功能多样性的影响[J]. 干旱区研究, 2022 , 39(5) : 1496 -1503 . DOI: 10.13866/j.azr.2022.05.14
Exploring the metabolic functional diversity of Medicago sativa rhizosphere microorganisms at different levels of phosphorus supply can further our understanding of the mechanism of efficient fertilizer use by M. sativa. Applying the biolog method through field experiments, we investigated the metabolic characteristics of the M. sativa rhizosphere microbial community under five different levels of phosphorus supply. The results showed that the application of phosphorus fertilizer could significantly improve the metabolic activity of M. sativa rhizosphere microorganisms; the Simpson and Richness indexes reached maximum values when the phosphorus supply level was P3 (300 kg·hm-2), which improved the utilization of different classified carbon sources by microorganisms. When the phosphorus fertilizer concentration was too high, it significantly reduced the Simpson index of the microbial community and also reduced the utilization of various carbon sources by microorganisms. Principal component analysis showed that there were significant differences in the utilization of different carbon sources by rhizosphere microorganisms at different levels of phosphorus supply. The smallest differences in the utilization of various carbon sources by the microbial community were observed at the highest level of phosphorus supply (P4, 450 kg·hm-2).
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