Euchoreutes naso is defined as an endangered species by International Union for Conservation of Nature (IUCN). It is important to do the research and protection with Euchoreutes naso. Study of its intestinal microbial diversity has highly conservation value. Seventeen sequences were obtained through extracting the total DNA, building 16S rRNA clone libraries, analyzing the nucleotide fragment length polymorphism which was digested by Endonuclease Bsur I within 262 clones and sequencing the different operational taxonomic units. After Basic Local Alignment Search Tool(BLAST) aligned, six orders of the gained species were obtained, which were Clostridiales, Lactobacillales, Enterobacteriales, Bacillales, Bacteroidales and Mycoplasmatales. Therefore, the phylogenetic tree could show a visual result.
〔1〕 马勇,李思华.长耳跳鼠一新亚种〔J〕.动物分类学报,1979,3(4):301-303.〔Ma Yong,Li Sihua.A new subspecies of the long-eared jerboa from Xinjiang〔J〕.Acta Zootaxonomica Sinica,1979,3(4):301-303.〕
〔2〕 王思博,杨赣源.新疆啮齿动物志〔M〕.乌鲁木齐:新疆人民出版社,1983.〔Wang Sibo,Yang Ganyuan.Zoography of Rodents in Xinjiang〔M〕.Urumqi:Xinjiang People’s Publishing House,1983.〕
〔3〕 高权新,吴天星,王进波.肠道微生物与寄主的共生关系研究进展〔J〕.动物营养学报,2010,22(3):519-526.〔Gao Quanxin,Wu Tianxing,Wang Jinbo.Advance in research on symbiotic relationship between intestinal bacterial and their host〔J〕.Chinese Journal of Animal Nutrition,2010,22(3):519-526.〕
〔4〕 王家沛.乌龟肠道微生物对鱼病的抑菌作用研究〔J〕.现代农业科技,2010(6):323-331.〔Wang Jiapei.Research of the turtle intestinal microbial imhibition to fish diseases〔J〕.Modern Agricultural Sciences and Technology,2010(6):323-331.〕
〔5〕 Zhu Y M,Luo T M,Jobin C,et al.Gut microbiota and probiotics in colon tumorigenesis〔J〕.Cancer Letters,2011,309(2):119-127.
〔6〕 Stephani J,Radulovic K,Niess J H,et al.Gut microbiota,probiotics and inflammatory bowel disease〔J〕.Archivum Immunologiae et Therapiae Experimentalis,2011,59(3):161-177.
〔7〕 Yang Y,Iji P A,Choct M.Dietary modulation of gut microflora in broiler chickens:A review of the role of six kinds of alternatives to infeed antibiotics〔J〕.World’s Poultry Science Journal,2009,65(3):97-111.
〔8〕 Ottaviani E,Ventura N,Mandrioli M,et al.Gut microbiota as a candidate for lifespan extension:An ecological/evolutionary perspective targeted on living organisms as metaorganisms〔J〕.Biogerontology,2011,12(6):599-609.
〔9〕 Ley R E,Hamady M,Lozupone C,et al.Evolution of mammals and their gut microbes〔J〕.Science,2008,320:1 647-1 651.
〔10〕 Torrazza R M,Neu J.The developing intestinal microbiome and its relationship to health and disease in the neonate〔J〕.Journal of Perinatology,2011,31:S29-S34.
〔11〕 凌泽春,郭立辉,任素芳,等.猪胃肠道微生物菌群的研究现状及调控技术进展〔J〕.家畜生态学报,2011(5):5-9.〔Ling Zechun,Guo Lihui,Ren Sufang,et al.Research progress of gastrointestinal microflora and the regulatory techniques in swine〔J〕.Acta Ecologiae Animalis Domastici,2011(5):5-9.〕
〔12〕 雷正瑜.16S rDNA序列分析技术在微生物分类鉴定中的应用〔J〕.湖北生态工程职业技术学院学报,2006,4(1):4-7.〔Lei Zhengyu.Application of 16S rDNA-sequential analysis in classification and determination of micro-orgnism〔J〕.Journal of Hubei Ecology Engineering College,2006,4(1):4-7.〕
〔13〕 张鹏.堆肥未培养微生物的宏基因组文库构建及新的木聚糖酶基因的克隆、鉴定和表达〔D〕.南宁:广西大学,2005:1-86.〔Zhang Peng.The Construction of A Metagenomic Library of Uncultured Bacteria from Compost and Cloning and Identification of Novel Xylanase Genes〔D〕.Nanning:Guangxi University,2005:1-86.〕
〔14〕 Gray J P,Herwig R P.Phylogenetic analysis of the bacterial communities in marine sediments〔J〕.Applied and Environmental Microbiology,1996,62(11):4 049-4 059.
〔15〕 Shiang R.Vector NTI Suite 2.0 v5.5.1〔J〕.Biotech Software & Internet Report:The Computer Software Journal for Scientists,2000,1(5):214-219.
〔16〕 Tamura K,Peterson D,Peterson N,et al.MEGA5:Molecular evolutionary genetics analysis using maximum likelihood,evolutionary distance,and maximum parsimony methods〔J〕.Molecular Biology and Evolution,2011,10(28):2 731-2 739.
〔17〕 高云,梁尚栋,穆松牛,等.调节肠道菌群动物模型和预防肠道菌群失调动物模型探讨〔J〕.江西医学院学报,2004,44(5):15-18.〔Gao Yun,Liang Shangdong,Mu Songniu,et al.Study of the animal models on regulation of intestinal flora and on prevention of its imbalance〔J〕.Acta Academiae Medicinae Jiangxi,2004,44(5):15-18.〕
〔18〕 Ramlachan N,Anderson R C.Characterization of an antibiotic resistant Clostridium hathewayi strain from a continuous-flow exclusion chemostat culture derived from the cecal contents of a feral pig〔J〕.Anaerobe,2007,13(3/4):153-160.
〔19〕 Ridlon J M,Hylemon P B.Identification and characterization of two bile acid coenzyme A transferases from Clostridium scindens,a bile acid 7 alpha-dehydroxylating intestinal bacterium〔J〕.Journal of Lipid Research,2012,53(1):66-76.
〔20〕 Ridlon J M,Kang D J,Hylemon P B.Isolation and characterization of a bile acid inducible 7 alpha-dehydroxylating operon in clostridium hylemonae TN271〔J〕.Anaerobe,2010,16(2):137-146.
〔21〕 Kycko A,Kozaczynski W,Jasik A,et al.Granulomatous pneumonia and hepatitis associated with Providencia rettgeri infection in a crocodile monitor lizard (Varanus salvadorii)〔J〕.Acta Veterinaria Hungarica,2013,61(1):51-58.
〔22〕 Iino T,Mori E A.Oscillibacter valericigenes gen.nov.,sp.nov.,a valerate-producing anaerobic bacterium isolated from the alimentary canal of a Japanese corbicula clam〔J〕.International Journal of Systematic and Evolutionary Microbiology,2007,57(8):1 840-1 845.
〔23〕 Ustiugova E A,Timofeeva A V,Stoianova L G,et al.Characteristics and identification of bacteriocins produced by Lactococcus lactis subsp.lactis 194-K〔J〕.Prikladnaia Biokhimiia i Mikrobiologiia,2011,48(6):618-625.
〔24〕 Akyol I.Regulation of the ribonucleotide reductases in Lactococcus lactis subsp.cremoris〔J〕.Acta Biologica Hungarica,2007,58(1):105-114.
〔25〕 Collins M D,Love D N,Karjalainen J,et al.Phylogenetic analysis of members of the genus porphyromonas and description of porphyromonas Cangingivalis sp.nov,and Porphyromonas cansulci sp.Nov〔J〕.International Journal of Systematic Bacteriology,1994,44(4):674-678.
〔26〕 Chaudet M M,Allen J L,Rose D R.Expression and purification of two family GH31 alpha-glucosidases from Bacteroides thetaiotaomicron〔J〕.Protein Expression Purification,2012,86(2):135-141.
