Plant Physiology

Physiological Response and Transcriptome of Hordeum brevisubulatum to Drought Stress

  • SONG Shi-wei ,
  • JIAO De-zhi ,
  • CHEN Xu ,
  • ZHAO Ze-long ,
  • YANG Yun-fei
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  • 1. Heilongjiang Key Laboratory of Resistance Gene Engineering and Preservation of Biodiversity in Cold Area,College of Life Science, Agriculture and Forestry,Qiqihar University,Qiqihar,161006,Heilongjiang,China;
    2. Key Laboratory of Vegetation Ecology under Ministry of Education,Institute of Grassland Science, Northeast Normal University,Changchun 130024,Jilin,China

Received date: 2018-10-30

  Revised date: 2019-01-12

  Online published: 2025-10-18

Abstract

The physiological response of Hordeum brevisubulatum to drought stress was researched so as to identify the drought tolerance related to the genes,physiological indexes and transcriptome sequencing of H.brevisubulatum seedlings under different concentrations of polyethylene glycol (PEG) 6000 stress.The results showed that the content of proline in leaves of H.brevisubulatum was increased continuously after the PEG 6000 stress for 2 days,and the activity of soluble sugar and superoxide dismutase (SOD) was increased at first and then decreased.The membership function method was used to select the transcriptome analysis and sequencing of H.brevisubulatum leaves under the treatment of 30 mmol·L-1 PEG 6000.Compared with the control group,the 6 868 up-regulated genes and 2 081 down-regulated genes were identified.The differentially expressed genes were classified into three categories and 54 functional groups by Go function classification.The 6 579 differentially expressed genes were enriched into 136 pathways by KEGG pathway enrichment.Arginine and proline metabolism,glycolysis and gluconeogenesis,starch and sucrose metabolism,peroxisome pathways and other pathways,and the related up-regulated expression genes in various metabolic pathways were discovered.

Cite this article

SONG Shi-wei , JIAO De-zhi , CHEN Xu , ZHAO Ze-long , YANG Yun-fei . Physiological Response and Transcriptome of Hordeum brevisubulatum to Drought Stress[J]. Arid Zone Research, 2019 , 36(4) : 909 -915 . DOI: 10.13866/j.azr.2019.04.15

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