利用花粉管通道法,获得的转Δ1-二氢吡咯-5-羧酸合成酶(Pyrroline-5-carboxylate synthetase,MvP5CS)高代棉花材料,比较田间干旱胁迫下转MvP5CS基因的T6代3个株系植株与受体棉花材料D5主要生理生化指标和农艺性状的差异。结果表明:从生理生化指标分析,转基因棉花比非转基因棉花植株更有利于在干旱胁迫条件下生长。从干旱胁迫后测量的农艺性状、产量性状和纤维品质的比较发现,转基因棉花植株的叶片颜色更绿;植株的主根更长,须根更多,更强壮;转基因棉花在单铃重、籽棉产量、皮棉产量、棉花衣分等性状明显优于非转基因棉花;纤维长度和强度略有增加,马克隆值降低,品质得到改善。
Using the method of pollen tube pathway, the high generation transferred MvP5CS (Pyrroline-5-carboxylate synthetase, MvP5CS) cotton material has been abtained.Comparing the main difference between the physiological and biochemical and difference in agronomic traits between the transfer MvP5CST6-generation cotton and non-genetically modified cotton under drought stress. The results show that: the transgenic cotton plants are more conducive to growth in the drought stress than non-transgenic cotton from analyzing the physiological and biochemical. After drought stress measurement of agronomic traits, yield traits and fiber quality: the color of the transgenic cotton leaves is greener and have longer taproot, stronger fibrous root. The transgenic cotton plants are significantly superior to the non-transgenic cotton in single boll weight, seed cotton yield, lint yield, fiber yield and other traits. Fiber length and strength increased slightly, incompatible micronaire is reduced, and quality gets improvement. This three transgenic cotton lines could provide new materials for the cotton resistance breeding.
〔1〕 买买提·莫明,李雪源,艾先涛,等.新疆棉花超高产育种技术初探〔J〕.中国棉花,2010,37(4):5-7.〔Maimaiti Moming,Li Xueyuan,Ai Xiantao,et al.Xinjiang cotton breeding technology〔J〕.China Cotton,2010,37(4):5-7.〕
〔2〕 赵振勇,乔木,吴世新,等.新疆耕地资源安全问题及保护策略〔J〕.干旱区地理,2010,33(6):1 019- 1 025.〔Zhao Zhenyong,Qiao Mu,Wu Shixin,et al.Cultivated land resource security and protection strategy in Xinjiang〔J〕.Arid Land Geography,2010,33(6):1 019-1 025.〕
〔3〕 王志才,廖茂森,徐建辉,等. 盐穗木耐盐基因通过花粉管通道法对棉花遗传转化的研究〔J〕.分子植物育种,2011,9(2):180-184.〔Wang Zhicai,Liao Maosen,Xu Jianhui,et al.Caspica salt-tolerant genes through pollen tube channel method to the study of cotton genetic transformation〔J〕.Molecular Plant Breeding,2011,9(2):180-184.〕
〔4〕 简纯平,李开绵,欧文军.花粉管通道法转基因育种研究进展〔J〕.热带作物学报,2012,33(5):956-961.〔Jian Chunpin,Li Kaimian,Ou Wenjun.Pollen tube channel method of transgenic breeding research progress〔J〕.Journal of Tropical Crops,2012,33(5):956-961.〕
〔5〕 刘冬梅,武芝霞,刘传亮,等.花粉管通道法获得棉花转基因株系主要农艺性状变异分析〔J〕.棉花学报,2007,19(6):450-454.〔Liu Dongmei,Wu Zhixia,Liu Chuanliang,et al.Pollen tube channel method for strain analysis of main agronomic characters of transgenic cotton〔J〕.Journal of Cotton,2007,19(6):450-454.〕
〔6〕 王志才,张富春.外源基因转化棉花育种研究的进展与应用〔J〕.生物技术通报,2011(2):12-17.〔Wang Zhicai,Zhang Fuchun.Exogenous gene into cotton breeding research progress and application〔J〕.Biological Technical Bulletin,2011(2):12-17.〕
〔7〕 周精华,邢虎成,揭雨成,等.苎麻Δ1-二氢吡咯-5-羧酸合成酶(P5CS)基因的克隆和表达分析〔J〕.作物学报,2012,38(3):549-555.〔Zhou Jinhua,Xin Hucheng,Jie Yucheng,et al.Molecular cloning and expression analysis of Δ1-Pyrroline-5-Carboxylate Synthetase(P5CS) Gene in Ramie〔J〕.Journal of Crops,2012,38(3):549-555.〕
〔8〕 张桦,张富春,曾光,等.新牧一号苜蓿MvP5CS基因的克隆和功能分析〔J〕.草业科学,2012,29(1):51-58.〔Zhang Hua,Zhang Fuchun,Zeng Guang,et al.New grazing alfalfa MvP5CS number one gene cloning and function analysis〔J〕.Pratacultural Science,2012,29(1):51-58.〕
〔9〕 杨云尧. 转MvP5CS1和MvNHXl基因提髙棉花抗旱性的研究〔D〕.乌鲁木齐:新疆农业大学,2012.〔Yang Yunyao.Research on High Cotton Drought Resistance of MvP5CS1 and MvNHXl genes〔D〕.Urumqi:Xinjiang Agricultural University,2012.〕
〔10〕李合生.植物生理生化实验原理和技术〔M〕.北京:高等教育出版社,2002.〔Li Hesheng.Principle and Technology of Plant Physiological and Biochemical Experiments〔M〕.Beijing:Higher Education Press,2002.〕
〔11〕高俊冯.植物生理学实验技术〔M〕.西安:世界图书出版公司,2000:194.〔Gao Junfeng.Plant Physiology Experiment Technology〔M〕.Xi'an:The World Book Publishing Company,2000:194.〕
〔12〕Hoekstrafa,Golovinaea,Buitink J.Mechanism of plant desic cation tolerance〔J〕.Treads Plant Science,2001,6:431-438.
〔13〕Kozi Asada.Production and scavenging of reactive oxygen species in chloroplasts and their functions〔J〕.Plant Physiology,2006,141:391-396.
〔14〕刘晓松.三叶草在干旱胁迫下的生理反应研究〔D〕.山东:山东师范大学,2009.〔Liu Xiaosong.Clover Physiological Reaction Research under Drought Stress〔J〕.Shandong:Shandong Normal University,2009.〕
〔15〕吴永美,烟章,王书建,等.植物抗旱生理生态特性研究进展〔J〕.粮食作物,2008,28(2):90-93.〔Wu Yongmei,Yan Zhang,Wang Shujian,et al.Plant drought resistance and physiological ecology characteristics is reviewed〔J〕.Food Crops,2008,28(2):90-93.〕
〔16〕培东,张喜英,王梭,等.谷子根系发育及其抗旱性研究〔J〕.中国生态农业学报,2002,10(4):28-30.〔Pei Dong,Zhang Xiyin,Wang Suo,et al.Corn root growth and drought resistance〔J〕.Journal of Chinese Ecological Agriculture,2002,10(4):28-30.〕
