采自额尔齐斯河(中国段)湖拟鲤(Rutilus rutilus lacustris Pallas)鳃部寄生的西伯利亚拟双身虫(Paradiplozoon homoion homoion Bychowsky et Nagibina,1959),系为我国首次报道,为新纪录。西伯利亚拟双身虫,隶属于扁形动物门(Playthelminthes),单殖吸虫纲(Monogenea),双身虫科(Diplozoidae),拟双身虫属(Paradiplozoon)。通过对西伯利亚拟双身虫的形态特征分析,发现西伯利亚拟双身虫与原始虫种相比仅部分结构大小略有差异,其他形态基本一致。同时,对西伯利亚拟双身虫ITS2 rDNA序列进行了测定,并将结果与GeneBank中已发表的序列进行同源性分析。结果表明,在额尔齐斯河采到的西伯利亚拟双身虫与GeneBank中同种ITS2 rDNA序列的同源性为100%。
It was the first time that Paradiplozoon homoion homoion Bychowsky et Nagibina, 1959 was found in China. Paradiplozoon homoion homoion Bychowsky et Nagibina, 1959, which was collected from the gills of Rutilus rutilus lacustris Pallas in the Irtysh River (Chinese section) in July 2010, belongs to Platyhelminthes, Monogenea, Diplozoinae, Paradiplozoon. Compared with the original Paradiplozoon homoion homoion Bychowsky et Nagibina, 1959, the morphological characteristics of the Paradiplozoon from the Irtysh River showed high similarities in basic morphological features but minor differences in structural sizes. The ITS2 rDNA sequence of the Paradiplozoon was tested and compared against the ITS2 rDNA sequences in Genebank for homogeneity. The results showed that the Paradiplozoon collected from the Irtysh River with a 100% of homogeneity in ITS2 rDNA sequence of the same species in Genebank.
〔1〕 任慕莲,郭焱,张人铭,等.中国额尔齐斯河鱼类资源及渔业〔M〕.乌鲁木齐: 新疆科技卫生出版社,2002:49-131.〔Ren Mulian,Guo Yan,Zhang Renming,et al.The Fish Resources and Fishery in Ergis River,China〔M〕.Urumqi:Health Science and Technology Publishing House in Xinjiang,2002:49-131.〕
〔2〕 吴宝华,郎所,王伟俊,等.中国动物志,扁形动物门,单殖吸虫纲〔M〕.北京:科学出版社,2000:635-674.〔Wu Baohua,Lang Suo,Wang Weijun,et al.Fauna China,Platyhelminthes,Monogenoidea〔M〕.Beijing:Science Press,2000:635-674.〕
〔3〕 Khotenovsky I A.Systematics and Phylgoeny of the families Dplozoidae and Discocotylidae(Monogenea)〔J〕.Parazitologia,1981,30:166-175.
〔4〕 中国科学院水生生物研究所鱼病学研究室.鱼病调查手册〔M〕.第2版.上海:上海科学技术出版社,1981.〔The Fish Disease Laboratory of Institute of Hydrobiology,Chinese Academy of Sciences.The Handbook of Fish Disease Examination〔M〕.2nd ed.Shanghai:Shanghai Science and Technology Press,1981.〕
〔5〕 湖北水生生物研究所.湖北省鱼病病原区系图志〔M〕.北京:科学出版社,1973.〔Hubei Institute of Hydrobiology.Hubei Fish Disease Pathogen Flora Disposition〔M〕.Beijin:Science Press,1973.〕
〔6〕 姜乃澄,吴宝华,王淑霞,等.我国双身虫亚科(Diplozoinae)吸虫的研究〔J〕.动物学报,1989,35(3):259-269.〔Jiang Naicheng,Wu Baohua,Wang Shuxia,et al.Studies on the trematode of subfamily Diplozoinae in China〔J〕.Acta Zoologica Sinica,1989,35(3):259-269.〕
〔7〕 Sambrook J,Russell D W.分子克隆实验指南〔M〕.黄培堂译.第3版.北京:科学出版社,2002.〔Sambrook J,Russell D W.Molecular Cloning:A Laboratory Manual〔M〕.3rd ed.Huang Peitang Translated.Beijing:Science Press,2002.〕
〔8〕 Gussev A V. Key to the Parasites of Freshwater Fish Fauna of the USSR〔M〕.Leningred:Science Publishing House,1985:1-425.
〔9〕 姜乃澄,吴宝华,王淑霞.浙赣两省寄生双身虫四新种〔J〕.动物分类学报,1985,10(2):113-118.〔Jiang Naicheng,Wu Baohua,Wang Shuxia.Four new species of Diplozoon from Zhejiang and Jiangxi Provinces in China〔J〕.Acta Zootaxonomica Sinica,1985,10(2):113-118.〕
〔10〕 夏晓勤,王伟俊,姚卫建.我国内陆水体单殖吸虫的宿主特异性分析〔J〕.生态学报,2000,20(4):594-597.〔Xia Xiaoqin,Wang Weijun,Yao Weijian.The host-specificity of monogeneans in inland waters of China〔J〕.Acta Ecologica Sinica,2000,20(4):594-597.〕
〔11〕 李生宇,雷加强.额尔齐斯河流域生态系统格局及变化〔J〕.干旱区研究,2002,19(2):56-61.〔Li Shengyu,Lei Jiaqiang.The ecosystem style and diversify of Ergis River drainage area〔J〕.Arid Zone Research,2002,19(2):56-61.〕
〔12〕 丁雪娟,廖翔华,李安兴,等.单殖吸虫分子系统学研究进展〔J〕.寄生虫与医学昆虫学,2005,12(2):112-118.〔Ding Xuejuan,Liao Xianghua,Li Anxing,et al.Advance in the studies of molecular systematics of monogenean〔J〕.Acta Parasitologica et Medica Entomologica Sinia,2005,12(2):112-118.〕
〔13〕 Ergens R,Gelnar M.Experimental verification of the effect of temperature on the size of the hard parts of haptor of Gyrodactylus katharineri Malmberg,1964 (Monogenea)〔J〕.Folia Parasitologica,1985,32:377-380.
〔14〕 Rohde K,Watson N.Morphology,microhabitats and geographical variation of Kuhnia spp.(Monogenea:Polyopisthocotylea)〔J〕.International Journal for Parasitology,1985,15:569-586.
〔15〕 Mo T A.Seasonal variations of the opisthaptoral hard parts of Gyrodactylus derjavini Mikailov,1975 (Monogenea:Gyrodactylidea) on brown trout Salmo trutta L.parr and Atlantic salmon S.salar L.parr in the River Sandvikselva,Norway〔J〕.Systematic Parasitology,1993,26:225-231.
〔16〕 Cunningham C O.Species variation within the internal transcribed spacer (ITS) region of Gyrodactylus(Monogenea:Gyrodactylidae)ribosomal RNA genes〔J〕.Journal of Parasitology,1997,83:215-219.
〔17〕 Cable L,Harris P D,Tinsley R C,et al.Phylogenetic analysis of Gyrodactylus spp.(Platyhelminthes:Monogenea) using ribosomal DNA sequences〔J〕.Canadian Journal of Zoology,1999,77:1 439-1 449.
〔18〕 Matejusova I,Koubkova B,Amelio S D,et al.Genetic characterization of six species of diplozoids (Monigenea:Diplozoidae)〔J〕.Parasitology,2001,123:465-474.