干旱区研究

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2014 , Vol. 31 >Issue 6: 1109 - 1114

DOI: https://doi.org/10.13866/j.azr.2014.06.19

生物资源

塔里木裂腹鱼群体的微卫星多态性分析

  • 杨天燕 ,
  • 孟玮 ,
  • 高天翔 ,
  • 郭焱 ,
  • 马燕武 ,
  • 张富春
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  • 1.中国海洋大学海洋生物多样性与进化研究所,山东 青岛 266003;
    2.新疆维吾尔自治区水产科学研究所,新疆 乌鲁木齐 830000;
    3.新疆大学生命科学与技术学院,新疆生物资源基因工程重点实验室,新疆 乌鲁木齐 830046
杨天燕(1982-),女,工程师,主要从事鱼类种群遗传学研究. E-mail:hellojelly1130@163.com
郭焱. E-mail:xjfishery@163.com

收稿日期: 2013-05-12

  修回日期: 2013-08-15

  网络出版日期: 2014-11-25

基金资助

新疆生物资源基因工程重点实验室开放课题(XJDX0201_2011_07);国家科技基础条件平台-水产种质资源平台项目;国家自然科学基金项目(31060356)

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Genetic Polymorphism of Microsatellite DNA in Schizothorax biddulphi Populations

  • YANG Tian-yan ,
  • MENG Wei ,
  • GAO Tian-xiang ,
  • GUO Yan ,
  • MA Yan-wu ,
  • ZHANG Fu-chun
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  • 1. Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, Shandong, China;
    2. Xinjiang Fisheries Research Institute, Urumqi 830000, Xinjiang, China;
    3. Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, Xinjiang, China

Received date: 2013-05-12

  Revised date: 2013-08-15

  Online published: 2014-11-25

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摘要

采用7对微卫星引物对塔什库尔干、多浪渠首、木扎提河、玉龙喀什河和喀拉喀什河5个群体共计114尾塔里木裂腹鱼遗传多样性进行比较分析。7对引物在5个群体中共扩增出34个条带,扩增片段在105~370 bp。计算群体间遗传距离发现,玉龙喀什河群体(Y)与塔什库尔干群体(T)间的遗传距离最大、与喀拉喀什河群体(K)间的遗传距离最小。基于遗传距离构建的UPGMA聚类树明显分为2支。塔里木裂腹鱼群体遗传多样性的AMOVA分析结果显示,群体内遗传变异为87.84%,群体间遗传变异为12.16%。整个种群F统计数Φst为0.121 61(P<0.01),群体间遗传分化显著,且塔什库尔干群体遗传多样度最高。

关键词： 塔里木裂腹鱼(Schizothorax biddulphi); 群体; 微卫星标记; 多态性; 遗传; 塔里木河; 新疆

本文引用格式

杨天燕 , 孟玮 , 高天翔 , 郭焱 , 马燕武 , 张富春 . 塔里木裂腹鱼群体的微卫星多态性分析[J]. 干旱区研究, 2014 , 31(6) : 1109 -1114 . DOI: 10.13866/j.azr.2014.06.19

Abstract

Population variations and genetic structures of five Schizothorax biddulphi populations (Tashikuergan, Duolang headworks, Muzhati River, Yulongkashi River, Kalakashi River) were analyzed using seven pairs of microsatellite primers. A total of 34 bands were amplified by all primers in five populations and the sizes of amplified fragments were from 105 bp to 370 bp. The genetic distance between Yulongkashi River population (Y) and Tashikuergan population (T) was the largest while the genetic distance between Yulongkashi River population (Y) and Kalakashi River population (K) was the smallest. UPGMA cluster tree which was constructed based on genetic distance was divided into two branches. The AMOVA results showed that variance within populations accounted for 87.84% and among populations accounted for 12.16%. The value of the F-statistic (Φst) was 0.121 61 (P<0.01) which meant there were significant genetic differentiations among populations, and the Tashikuergan population had the highest genetic diversity.

Key words： Schizothorax biddulphi; population; microsatellite marker; polymorphism; genetic; Tarim River; Xinjiang

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