干旱区研究

干旱区研究 >

2025 , Vol. 42 >Issue 5: 885 - 894

DOI: https://doi.org/10.13866/j.azr.2025.05.11

植物生态

基于RT-qPCR技术解析齿肋赤藓耐热基因在不同非生物胁迫下的表达模式

  • 霍雯婷 ,
  • 顾天麒 ,
  • 高梦宇 ,
  • 宋艳芳 ,
  • 李鸿彬 ,
  • 卓露
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  • 1.石河子大学生命科学学院， 新疆植物药资源利用教育部重点实验室，绿洲城镇与山盆系统生态兵团重点实验室， 新疆 石河子 832000
    2.石河子卫生学校， 新疆 石河子 832000
霍雯婷（1997-），女，硕士研究生，主要从事荒漠藓类植物抗逆生理及分子生物学研究. E-mail: huowenting@stu.shzu.edu.cn
卓露. E-mail: Luzhuo@shzu.edu.cn

收稿日期: 2025-01-10

  修回日期: 2025-03-26

  网络出版日期: 2025-10-22

基金资助

国家自然科学基金项目(32360097);2023年兵团发展专项-博士后专项项目(CZ00150102)

收起

Analysis of the expression patterns of the heat-resistant gene of Syntrichia caninervis under different abiotic stresses based on RT-qPCR technology

  • HUO Wenting ,
  • GU Tianqi ,
  • GAO Mengyu ,
  • SONG Yanfang ,
  • LI Hongbin ,
  • ZHUO Lu
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  • 1. College of Life Science, Shihezi University, Ministry of Education Key Laboratory of Xinjiang Phytomedicine Resource Utilization, Xinjiang Production and Construction Corps Key Laboratory of Oasis Town and Mountain-basin System Ecology, Shihezi 832000, Xinjiang, China
    2. Shihezi Health School, Shihezi 832000, Xinjiang, China

Received date: 2025-01-10

  Revised date: 2025-03-26

  Online published: 2025-10-22

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摘要

依托前期55 ℃高温胁迫下耐干苔藓齿肋赤藓的转录组数据，运用实时荧光定量聚合酶链式反应（Quantitative Real time Polymerase Chain Reaction，RT-qPCR)技术，探究9个高温差异表达基因（ScLEA14、ScGSTF11、ScHSP70-17、ScHsfB4b、ScMYB117、ScGLK1、ScERF039、ScERF016、ScbHLH104）在高温、干旱-复水及NaCl胁迫下的表达特征，以此验证转录组测序（RNA sequencing，RNA-Seq）数据的可靠性，为后续齿肋赤藓抗逆基因功能验证提供理论支撑。结果显示：（1） 9个基因在高温胁迫下的表达趋势与RNA-Seq数据基本一致。（2） 在极端高温和干旱-复水胁迫时，9个基因均被不同程度诱导，其中3个基因在干旱24 h时诱导至表达峰值，8个基因在复水阶段诱导效果更为突出。（3） 在NaCl盐胁迫下，9个耐热相关基因均受盐诱导而上调表达，其中6个基因表现尤为显著。由此得出：ScLEA14、ScMYB117、ScERF016这3个基因在极端高温、干旱-复水和NaCl高盐胁迫下均显著诱导表达，可作为后续抗逆研究的候选基因。

关键词： 耐干苔藓; 齿肋赤藓; 高温胁迫; 转录组; 实时荧光定量PCR

本文引用格式

霍雯婷 , 顾天麒 , 高梦宇 , 宋艳芳 , 李鸿彬 , 卓露 . 基于RT-qPCR技术解析齿肋赤藓耐热基因在不同非生物胁迫下的表达模式[J]. 干旱区研究, 2025 , 42(5) : 885 -894 . DOI: 10.13866/j.azr.2025.05.11

Abstract

Based on transcriptomic data of the desiccation-tolerant moss Syntrichia caninervis under prior 55 ℃ heat stress, this study employed real-time fluorescent quantitative PCR (RT-qPCR) to investigate the expression patterns of nine heat-responsive differentially expressed genes (ScLEA14, ScGSTF11, ScHSP70-17, ScHsfB4b, ScMYB117, ScGLK1, ScERF039, ScERF016, and ScbHLH104) under high temperature, drought-rehydration, and NaCl stress conditions. The aim was to validate the reliability of RNA-Seq data and provide theoretical support for subsequent functional studies on stress-resistant genes in S. caninervis. Results demonstrated that: (1) The expression profiles of all nine genes under high-temperature stress exhibited substantial concordance with RNA-Seq data, confirming the stability of transcriptomic sequencing. (2) Under extreme heat and drought-rehydration stresses, all genes were differentially induced, with three genes attaining peak expression levels following 24-hour drought treatment, while eight genes displayed more prominent transcriptional activation during the rehydration phase. (3) NaCl stress triggered significant upregulation of all nine thermotolerance-associated genes, with six genes demonstrating statistically robust induction. Thus, the results demonstrate that the three genes ScLEA14, ScMYB117, and ScERF016 are strongly induced under extreme high temperature, drought-rehydration, and NaCl-induced high salinity stress, highlighting their potential as key candidate genes for further investigation into stress resistance mechanisms.

Key words： desiccation moss; Syntrichia caninervis; high temperaturestress; transcriptome; RT-qPCR

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