干旱区研究

干旱区研究 >

2025 , Vol. 42 >Issue 3: 499 - 510

DOI: https://doi.org/10.13866/j.azr.2025.03.10

植物生态

基于SSR分子标记的5个黄花补血草居群花型变异及其遗传效应

  • 张晶 ,
  • 何爽 ,
  • 张爱勤
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  • 新疆大学生命科学与技术学院，新疆生物资源与基因工程重点实验室，新疆 乌鲁木齐 830017
张晶（1996-），女，硕士研究生，主要从事植物繁殖生态学研究. E-mail: 2276834219@qq.com
张爱勤. E-mail: zhangaq@xju.edu.com

收稿日期: 2024-01-09

  修回日期: 2024-06-09

  网络出版日期: 2025-03-17

基金资助

国家自然科学基金项目(32360308);自治区教育厅项目(XJEDU2021I006)

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Floral morph variation and genetic effect of five Limonium aureum populations based on Simple Sequence Repeat molecular markers

  • ZHANG Jing ,
  • HE Shuang ,
  • ZHANG Aiqin
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  • Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830017, Xinjiang, China

Received date: 2024-01-09

  Revised date: 2024-06-09

  Online published: 2025-03-17

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摘要

异型花柱植物居群的花型构成与频率是历代交配事件的结果。“同型花柱”是异型花柱变异和演化中频繁出现的一个的花型，常伴随着异型不亲和系统的瓦解和居群遗传多样性的下降。以分布于塔里木盆地南缘，具有类似于“同型花柱”型花（H型）广泛分布的5个黄花补血草（Limonium aureum）居群为研究对象，来探究H型花的形成及对居群产生的影响。通过野外观测及人工控制授粉实验，对居群花型构成和异型花柱综合征进行调查，同时通过SSR分子标记对居群的遗传多样性和遗传结构进行研究。结果表明：（1） ATS居群仅由H型花构成，其余均为长、短花柱和H型花共存的居群，各居群均具有中等程度的遗传多样性水平、花粉柱头形态的二型性和严格的异型不亲和系统，表现为柱头/花粉形态具有异型性的花型间亲和，反之不亲和。（2） 遗传结构、主坐标分析及系统发育分析均显示ATS居群在进化上独立于其余4个居群，5个居群被分为两类，且遗传距离和地理距离呈显著相关性。（3） 两类居群中的H型花可能处于异型花柱演化的不同阶段，其严格的自交不亲和性维持了各居群的遗传多样性水平。

关键词： 异型花柱; 黄花补血草; 花型变异; 遗传多样性; 同长花柱

本文引用格式

张晶 , 何爽 , 张爱勤 . 基于SSR分子标记的5个黄花补血草居群花型变异及其遗传效应[J]. 干旱区研究, 2025 , 42(3) : 499 -510 . DOI: 10.13866/j.azr.2025.03.10

Abstract

In heterostylous plants, the floral morph composition and frequency of populations are a consequence of mating events over generations. “Homostyly” with anthers and stigmas at the same level within a flower is a floral morph that frequently appears in the evolution of heterostyly and is often accompanied by breakdown of the heteromorphic incompatibility system and the decline of genetic diversity. To explore the formation of the H-morph and its effects on the population, we studied five Limonium aureum populations exhibiting a widely distributed floral morph (H-morph), similar to “homostyly,” in the southern margin of Tarim Basin. The floral morph composition and heterostylous syndrome were investigated by field observations and hand pollination experiments. Meanwhile, the genetic diversity and genetic structure were studied using SSR molecular markers. The results showed the following: (1) The Atushi (ATS) population consisted only of the H-morph, while the remaining four populations were composed of the long- and short-styled morph, and H-morph. All populations had a moderate level of genetic diversity, dimorphism of stigma-pollen morphology, and a strict heteromorphic incompatibility system, revealing that the floral morphs with different pollen ornamentation and stigma papilla cell morphology were compatible. (2) Genetic structure analysis, principal coordinate analysis, and phylogenetic analysis showed that the ATS population had emerged earlier and was independent of the other four populations phylogenetically, with a significant correlation between genetic distance and geographic distance. (3) The H-morphs of the two types of populations may be at different stages of the evolution of heterostyly. The self-incompatibility of the H-morph and the heteromorphic incompatibility system of the population maintained the population’s genetic diversity.

Key words： heterostyly; Limonium aureum; floral variation; genetic diversity; homostyly

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